| 培养基 Medum | 作用 Function | 一次继代培养时间 Culturing time/d | 继代次数 Subculturing times |
|---|---|---|---|
| M0 | 无菌苗培养 Culturing sterile seedling | 5 | 1 |
| M2 | 共培养 Co-cultivation | 2 | 1 |
| M3 | 筛选培养 Callus induction and selection | 30（暗培养）+30（光培养） | 1 |
| M3 | 筛选培养 Callus induction and selection | 30 | 0~1 |
| M4 | 筛选培养 Callus induction and selection | 25~30 | 1 |
| M1 | 增殖培养 Callus proliferation | 30~40 | 2~3 |
| M5 | 分化培养 Callus differentiation | 20 | 2~6 |
| M6 | 分化培养 Callus differentiation | 25 | 1~2 |
| M7 | 成苗 Culturing regenerated plantlet | 30 | 1~2 |
