| 培养基 Medium | 组成成分 Components |
|---|---|
| MS | Murashige and Skoog培养基(M519, PhytoTechnology Laboratories,美国) Murashige and Skoog medium(M519, PhytoTechnology Laboratories,USA) |
| M0 | 1/2 MS+20 g·L-1葡萄糖+3.0 g·L-1植物凝胶, pH 6.5 1/2 MS+20 g·L-1 glucose + 3.0 g·L-1 phytagel, pH 6.5 |
| M1 | MS无机盐(硝酸钾加倍)+10 mg·L-1维生素B1+30 g·L-1葡萄糖+3.0 g·L-1 植物凝胶, pH 6.8 MS mineral salt(doubled potassium nitrate) + 10 mg·L-1 vitamin B1 + 30 g·L-1 glucose + 3.0 g·L-1 phytagel, pH 6.8 |
| M2 | M1 + 0.1 mg·L-1 2,4-D* + 0.1 mg·L-1 KT** |
| M3 | M2培养基+100 mg·L-1卡那霉素+500 mg·L-1头孢霉素 M2 medium + 100 mg·L-1 kanamycin + 500 mg·L-1 cefotaxim |
| M4 | M1培养基+100 mg·L-1卡那霉素+500 mg·L-1头孢霉素 M1 medium + 100 mg·L-1 kanamycin + 500 mg·L-1 cefotaxim |
| M5 | M1培养基(去掉硝酸铵,植物凝胶)+0.5 g·L-1天冬酰胺+1.0 g·L-1谷氨酰胺 M1 medium (without potassium nitrate and phytagel) + 0.5 g·L-1 asparagine + 1.0 g·L-1 glutamine |
| M6 | M1培养基(去掉硝酸铵,植物凝胶,葡萄糖)+0.5 g·L-1天冬酰胺+1.0 g·L-1谷氨酰铵 M1 medium (without potassium nitrate, phytagel, and glucose) + 0.5 g·L-1 asparagine + 1.0 g·L-1 glutamine |
| M7 | M1培养基(去掉硝酸铵,植物凝胶,葡萄糖)+0.5 g·L-1天冬酰胺+1.0 g·L-1谷氨酰铵 M1 medium (without potassium nitrate, phytagel, and glucose) + 0.5 g·L-1 asparagine + 1.0 g·L-1 glutamine |