以自主选育的7个显性无腺体新品系为材料,通过种子性状考察,种仁游离棉酚、脂肪酸和氨基酸含量测定,研究了低酚棉种仁具有重要的油用、食用价值和保健功能。结果表明:4个新品系种仁棉酚含量低于国家食用标准,其中苏显无154、苏显无156棉酚占种仁质量低于3 mg·kg-1,成为名副其实的无酚棉新种质。低酚新品系种仁粗脂肪含量高于有酚品种,不饱和脂肪酸总含量超过70%,油酸相对含量略低于有酚品种,亚油酸、亚麻酸、棕榈油酸相对含量以及不饱和脂肪酸总含量与有酚品种相同。低酚新品系种仁色氨酸含量高于常规有酚品种,其它必需氨基酸含量以及氨基酸总含量与有酚品种相近。低酚基因Gl2e对棉花种仁脂肪酸、氨基酸组成及其含量未产生负效应。
Abstract
In order to clarify the oil, edible value and healthy functions of kernels from new low-gossypol strains, seven new dominant glandless strains and two gossypol cultivars from upland cotton, Sumian 22 and Sikang 1, were selected to determine content of free gossypol, fatty acids, amino acids in kernels, and other characters. The results showed that the gossypol content of the kernels of four new strains were under the national edible standard (200 mg·kg-1). Suxianwu 154 and Suxianwu 156 are new upland cotton lines without gossypol. The rough fat content of kernels from new low-gossypol strains were higher than that from gossypol cultivars, and the total content of unsaturated fatty acids from low gossypol kernels was more than 70%. The relative amounts of linoleic acid, linolenic acid, palm oleic acid, and total unsaturated fatty acids from low gossypol kernels were the same as that from gossypol ones, whereas the relative amounts of oleic acid from low gossypol kernels were slightly lower than that from gossypol ones. Tryptophan content of low gossypol kernels was significantly higher than that of conventional gossypol kernels, and the content of other essential amino acids as well as the total content of amino acids from low gossypol kernels were the same as that from gossypol ones. There were no adverse influences of the glandless gene Gl2e on the composition and content of fatty acids and amino acids in low gossypol kernels.
关键词
棉花 /
显性低酚 /
种仁 /
脂肪酸 /
氨基酸
{{custom_keyword}} /
Keywords
cotton /
dominant low gossypol /
kernel /
fatty acids /
amino acids
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 中国农业科学院棉花研究所.中国棉花遗传育种学[M].济南:山东科学技术出版社,2009:561-582. Institute of Cotton Research, Chinese Academy of Agricultural Sciences. Chinese cotton genetics and breeding[M]. Ji'nan: Shandong Science and Technology Publishing House, 2009: 561-582.
[2] 祝水金,汪若海,王红梅,等.低酚棉新品种—中棉所22
[J].中国棉花,1994,21(12):23-24. ZHU Shui-jin, Wang Ruo-hai, Wang Hong-mei, et al. CCRI 22, an upland cotton new cultivar with low gossypol content
[J]. China Cotton, 1994, 21(12): 23-24.
[3] 周安定,薛至祥.抗病低酚棉晋棉14号的选育及应用
[J].山西农业科学,1994,22(1):29-32. ZHOU An-ding, Xue Zhi-xiang. Selection, breeding and utilization of Jinmian No.14, an upland cotton new cultivar with Fusarium resistance and low gossypol content
[J]. J Shanxi Agricultural Sciences, 1994,22(1):29-32.
[4] 邱新棉.高产优质抗病低酚棉—浙棉10号
[J].中国农学通报,1995,11(6):50. QIU Xin-mian. Zhemian 10, an upland cotton new cultivar with high lint yield, good fiber quality, Fusarium resistance and low gossypol content
[J]. Chinese Agronomy Bulletin, 1995, 11(6): 50.
[5] 杨效奎,梁小东,黄卫华,等.皖棉18特征特性及高产栽培技术
[J].安徽农业,2004(11):22. YAN Xiao-kui, Liang Xiao-dong, Huang Wei-hua, et al. The characteristic, specific property and high-yield culture technique of Wanmian 18, an upland cotton new cultivar with Fusarium resistance and low gossypol content
[J]. Anhui Agriculture, 2004(11): 22.
[6] DOBBS J H, Oakley S R. CPCSD Acala GLS: a glandless cotton variety for the San Joanquin Valley
[C]. //Proceedings of the Beltwide Cotton Conference, San Antonio, January 4-8,2000. Texas:National Cotton Council of USA, 2000, I: 115.
[7] ROMANO G B, Scheffler J A. Lowering seed gossypol content in the glanded cotton(Gossypium hirsutum L.)
[J]. Plant Breeding, 2008, 127(6): 619-624.
[8] MANSOUR M H, Zohdy N M, El-Gengaihi S, et al. The status of gossypol and tannin contents in some Egyptian cotton cultivars in correlation bollworm infestation
[J]. Bulletin of the National Research Centre (Cario), 2008, 29(1): 121-128.
[9] 杨 艳,王贤磊,李 冠.六种新疆陆地棉棉籽脂肪酸成分分析
[J].生物技术,2009,19(4):54-56. YANG Yan, Wang Xian-lei, Li Guan. Analysis on fatty acid composition of six species of upland cotton
[J]. Biotechnology, 2009, 19(4): 54-56.
[10] MCMICHAEL S C. Combined effects of the glandless genes gl2 and gl3 on pigment glands in the cotton plant
[J]. Agron J, 1960, 52: 385-386.
[11] AFIFI A, Bary A A, Kamel S A, et al. Bahtim 110, a new strain of Egyptian cotton free from gossypol
[J]. Emp Cot Grow Rev,1966, 43(2): 112-120.
[12] 邱新棉.试论低酚棉资源及其利用前景
[J].中国棉花,2000,27(2):7-9. QIU Xin-mian. The germplasms of low gossypol upland cotton and their utilization prospect
[J]. China Cotton, 2000, 27(2):7-9.
[13] 韩 菊,魏福祥,庞津霞,等.转Bt基因棉籽仁中营养成分的研究
[J].中国油脂,2003,28(10):58-60. HAN Ju, Wei Fu-xiang, Pang Jin-xia, et al. Nutritional ingredients in transgenic Bt cotton seed kernel
[J]. Chinese Lipa, 2003, 28(10): 58-60.
[14] 韩 菊,冯 冬,何春林,等.低酚棉籽仁中脂肪酸成分的研究
[J].分析测试学报,1997,16(2):19-21. HAN Ju, Fong Dong, He Chun-lin, et al. Study on the composition of fatty acids in glandless cottonseeds
[J]. Journal of Instrumental Analysis, 1997, 16(2): 19-21.
[15] 赵冬冬,刘晓宇.棉籽蛋白的研究进展
[J].农产品加工·学刊,2009(5):27-30. ZHAO Dong-dong, Liu Xiao-yu. Advances of research on cottonseed protein
[J]. Academic Periodical of Farm Products Processing, 2009(5): 27-30.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家“十二五”转基因生物新品种培育科技重大专项(2011ZX08005-001);江苏省农业科技自主创新资金项目 [cx (10) 135];江苏省“十二五”科技支撑计划项目(BE2011304)
{{custom_fund}}