Cotton Science

Genetic diversity and haplotype analysis of cotton Fusarium wilt based on gene sequences of EF-1α and β-tubulin

Wang Haiying, Chen Xiaohai, Zhong Yeyi, Gong Juwu, Liu Ping, Chin Yaoxian, Wang Peizheng, Yuan Youlu

Cotton Science, 2023, 35(4): 334-344. DOI: 10.11963/cs20220060

单倍型 Haplotype	菌株 Strain	序列数 Sequence number	序列数量占比 Proportion of sequence number/%
H1	SHIBU X14, SHIBU W9, SHIBU V2, SHIBU W6, SHIBU W2, Race1 ATCC16421	6	23.08
H2	Race1 ATCC46644, Race2 ATCC16611(1), Race3 CA3	3	11.54
H3	Race3 ATCC16612	1	3.85
H4	Race4 ATCC16613, Race4 CA14, Race6 ATCC36198, Race8 CA1	4	15.38
H5	Aus Type Aust 16, Race8 ATCC31665	2	7.69
H6	Aus Type Aust 19	1	3.85
H7	Race8 CN8, Aus Type ATCC96291, HB114, HB48, HB7, HB70, HB8-2	7	26.92
H8	HB120	1	3.85
H9	HB180-2	1	3.85

Table 4 Haplotype analysis based on β-tubulin gene sequence

Other figure/table from this article

Table 1 GenBank accession number of EF-1α and β-tubulin gene of cotton Fusarium wilt
Fig. 1 Phylogenetic tree based on EF-1α gene sequence of cotton Fusarium wilt strains The value on each branch is the degree of genetic variation, which indicates the frequency of base replacement at each site in the gene. The calculation formula is: degree of variation = the number of mutated bases/the total number of bases.
Fig. 2 Phylogenetic tree based on β-tubulin gene sequence of cotton Fusarium wilt strains The value on each branch is the degree of genetic variation, which indicates the frequency of base replacement at each site in the gene. The calculation formula is: degree of variation = the number of mutated bases/the total number of bases.
Table 2 Haplotype characteristics of EF-1α and β-tubulin gene
Fig. 3 Haplotype network map constructed based on EF-1α gene sequence The number of short horizontal lines indicates the number of mutated bases between two haplotypes.
Table 3 Haplotype analysis based on EF-1α gene sequence
Fig. 4 Haplotype network map constructed based on β-tubulin gene sequence The number of short horizontal lines indicates the number of mutated bases between two haplotypes.