The aim of this study was to induce embryogenic callus from various cultivars of cotton in tissue culture, so that a stable and efficient regeneration system could be developed to produce new cotton varieties for cultivation in Xinjiang. The explant materials were hypocotyls of the main cotton cultivars grown in Xinjiang, i. e. Xinhai 25, Xinhai 16, Xinluzao 39, and Xinluzao 42. We tested the effects of different combinations of two hormones (kinetin, KT; 2,4-dichlorophenoxyacetic acid, 2,4-D) on induction of callus from these explants. Calli were produced by the explants under four different combinations of hormones in the media. The optimal hormone combination to induce callus from Gossypium hirsutum explants was 0.1 mg·L-1 KT + 0.05 mg·L-1 2,4-D, while that to induce callus from Gossypium barbadense explants was 0.1 mg·L-1 KT + 0.1 mg·L-1 2,4-D. Hormone-free medium and medium containing double to the normal concentration of KNO3 promoted the emergence of embryogenic callus. Filter paper placed under the medium promoted somatic embryo growth and regeneration of the root system. The differentiation and embryogenesis processes occurred more rapidly in G. hirsutum explants than those in G. barbadense explants. Using this protocol, normal plantlets of these cotton cultivars with strong roots were produced within 10 to 12 months. These methods could be used to increase the number of cotton genotypes that can be regenerated in tissue culture.
A Yi-Xia-Mu-Gu-Li-·Si-Ma-Yi-Li, QU Yan-Ying, SE Na-Wa-Er-·Mai-Mai-Ti-Ming, MENG Ling-Zhen, YANG Ting, WANG Li-Ping, CHEN Quan-Jia- .
Induction of Embryogenic Callus in Gossypium barbadense L. and Gossypium hirsutum L. by Hormone Treatments[J]. Cotton Science, 2013, 25(4): 352-358. https://doi.org/10.11963/cs130411
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