毛棉苗期抗旱性状的QTL定位

doi:10.11963/issn.1002-7807.201701004

摘要/Abstract

摘要： 目的通过分析毛棉苗期抗旱相关性状的数量性状位点（Quantitative trait locus,QTL）,以期检测稳定的主效QTL,促进栽培品种抗旱性状遗传改良及提高抗旱育种效率。方法以四倍体野生种毛棉（Gossypium tomentosum）和陆地棉品种中棉所12（CCRI 12)的种间杂种F₂及其F_2:3家系为研究材料,用于基因型分型的F₂有188个系,用于表型分型的F_2:3家系有149个株系。分别在干旱胁迫和正常灌水2个环境下调查表型数据。采用复合区间作图法对F_2:3家系苗期相关性状抗旱系数进行QTL定位。结果对苗期相关性状抗旱系数的QTL定位分析,共得到16个QTL,其中与株高、叶片数、叶绿素含量、脯氨酸含量、丙二醛含量抗旱系数相关的QTL分别有5个、1个、3个、3个、4个,分布在13条染色体上。来自毛棉的5个加性QTL分别为qSHDC-19-1、qSHDC-19-2、qSLNDC-5-1、qMDADC-24-1、qMDADC-24-2,其加性效应值为0.10~0.22,解释变异9.4%~25.8%。结论这些与抗旱相关的QTL有助于棉花抗旱分子标记辅助选择。

关键词: 毛棉; 种间杂交; 苗期; 抗旱; 数量性状位点

Abstract:

[Objective] The aim of this study was to reveal the genetic basis of drought tolerance traits and detect major quantitative trait loci (QTLs) by mapping drought tolerance QTLs on the Gossypium tomentosum genome. This information may be useful for developing drought-tolerant cotton cultivars. [Method] The present study examined the genetic basis of drought tolerance in 188 F₂ lines and 149 F_2:3 lines developed from an interspecific cross between a wild cotton species, Gossypium tomentosum, and an upland cotton variety (CCRI 12). Five morpho-physiological traits (i.e., height, number of leaves, and chlorophyll, malondialdehyde, and proline contents) were assessed under water-limited (W1) and well-watered (W2) conditions during the seedling stage. Additionally, the composite interval mapping method was used for QTL mapping of drought resistance coefficients for the abovementioned traits. [Results] Sixteen significant QTLs were detected on 13 chromosomes in the F_2:3 population. We detected five QTLs for plant height, one for the number of leaves, three for chlorophyll content, three for leaf malondialdehyde content, and four for proline content. The qSHDC-19-1, qSHDC-19-2, qSLNDC-5-1, qMDADC-24-1, and qMDADC-24-2 alleles were derived from G. tomentosum, and explained 9.4%-25.8% of the phenotypic variation. [Conclusion] These results may help elucidate the genetic basis of drought tolerance in cotton, and may be important for breeding programs involved in marker-assisted development of new cultivars with improved tolerance to drought stress.

Key words: Gossypium tomentosum; inter-specific hybridization; seedling stage; drought resistance; quantitative trait locus

郑巨云, George Oluoch, Khan Muhammad Kashif Riaz, 周忠丽, 王星星, 蔡小彦, 李雪源, 王春英, 王玉红, 刘方, 王坤波. 毛棉苗期抗旱性状的QTL定位[J]. 棉花学报, 2017, 29(1): 29-39.

Zheng Juyun, George Oluoch, Khan Muhammad Kashif Riaz, Zhou Zhongli, Wang Xingxing, Cai Xiaoyan, Li Xueyuan, Wang Chunying, Wang Yuhong, Liu Fang, Wang Kunbo. Mapping QTLs for Traits Related to Drought Tolerance at the Seedling Stage in an Inter-Specific Gossypium hirsutum × Gossypium tomentosum F_2:3 Population[J]. Cotton Science, 2017, 29(1): 29-39.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/issn.1002-7807.201701004

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2017/V29/I1/29

参考文献

[1]	李东晓. 干旱对棉花叶片的衰老生理及抗氧化酶同工酶谱特征的影响[D]. 保定: 河北农业大学, 2010.
[1]	Li Dongxiao.The effect of drought on premature senescence physiology and isozyme spectrum of antioxidant enzyme of cotton leaves[D]. Baoding: Agricultural University of Hebei, 2010.
[2]	李秉柏, 陆景淮, 吴金栋. 旱涝灾害对江苏棉花生产影响初探[J]. 中国农业气象, 1995, 16(3): 23-26.
[2]	Li Bingbai, Lu Jinghuai, Wu Jindong.A primary study on the effects of drought and waterlogging on cotton production in Jiangsu[J]. China Meteorological, 1995, 16(3): 23-26.
[3]	Jiang Yiwei, Huang Bingru.Effects of drought or heat stress alone and in combination on Kentucky blue grass[J]. Crop Science, 2000, 40: 1358-1362.
[4]	Huang B, Fry J D.Root anatomical, physiological, and morphological responses to drought stress for tall fescue cultivars[J]. Crop Science, 1998, 38: 1017-1022.
[5]	Jiang Yiwei, Huang Bingru.Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation[J]. Crop Science, 2001, 41: 436-442.
[6]	Xu Qingzhang, Huang Bingru.Lowering soil temperatures improves creeping bentgrass growth under heat stress[J]. Crop Science, 2001, 41: 1878-1883.
[7]	俞希根, 孙景生, 肖俊夫, 等. 棉花适宜土壤水分下限和干旱指标研究[J]. 棉花学报, 1999, 11(1): 35-38.
[7]	Yu Xigen, Sun Jingsheng, Xiao Junfu, et al.A study on drought indices and lower limit of suitable soil moisture of cotton[J]. Acta Gossypii Sinica,1999, 11(1): 35-38.
[8]	杜传莉, 黄国勤. 棉花主要抗旱鉴定指标研究进展[J].中国农学通报, 2011, 27(9): 17-20.
[8]	Du Chuanli, Huang Guoqin.Research progress of major identification indicators in the cotton drought resistance[J]. Chinese Agricultural Science Bulletin, 2011, 27(9): 17-20.
[9]	倪郁, 李唯. 作物抗旱机制及其指标的研究进展与现状[J]. 甘肃农业大学学报, 2001, 36(1): 14-22.
[9]	Ni Yu, Li Wei.The status and recent development in crop drought-resistant mechanism and index[J]. Journal of Gansu Agricultural University, 2001, 36(1): 14-22.
[10]	冯方剑, 宋敏, 陈全家, 等. 棉花苗期抗旱相关指标的主成分分析及综合评价[J]. 新疆农业大学学报, 2011, 34(3): 211-217.
[10]	Feng Fangjian, Song Min, Chen Quanjia, et al.Analysis and comprehensive evaluation on principal component of relative indices of drought resistance at the seedling stage of cotton[J]. Journal of Xinjiang Agricultural University, 2011, 34(3): 211-217.
[11]	冀天会, 张灿军, 谢惠民, 等. 小麦品种抗旱性鉴定产量指标的比较研究[J]. 中国农学通报, 2006, 22(1): 103-106.
[11]	Ji Tianhui, Zhang Canjun, Xie Huimin, et al.A comparative study on yield index of wheat varieties drought resistance[J]. Chinese Agricultural Science Bulletin, 2006, 22(1): 103-106.
[12]	王海标, 陈全家, 刘鹏鹏, 等. 苗期干旱胁迫对棉花生理特性、产量构成因素和纤维品质的影响[J]. 新疆农业科学, 2013, 50(12): 2172-2181.
[12]	Wang Haibiao, Chen Quanjia, Liu Pengpeng, et al.Effect of drought during seedling stage on physiological traits, yield components and fiber quality of 4 kinds of cotton[J]. Xinjiang Agricultural Sciences, 2013, 50(12): 2172-2181.
[13]	Said J I, Lin Zhongxu, Zhang Xianlong. A comprehensive meta QTL analysis for fiber quality, yield, yield related and morphological traits, drought tolerance, and disease resistance in tetraploid cotton[J/OL]. BMC Genomics, 2013, 14: 776 [2016-02- 18]. . DOI:10.1186/1471-2164-14-776.
[14]	郭纪坤. 陆地棉抗旱耐盐及产量、形态性状的QTL定位[D]. 乌鲁木齐: 新疆农业大学, 2007.
[14]	Guo Jikun.QTLs associated with drought tolerance, salt resistance, yield and morphological traits in upland cotton[D]. Wulumuqi: Xinjiang Agricultural University, 2007.
[15]	Saeed M, Guo Wangzhen, Ullah I, et al.QTL mapping for physiology, yield and plant architecture traits in cotton (Gossypium hirsutum L.) grown under well-watered versus water-stress conditions[J]. Electronic Journal of Biotechnology, 2010, 14(3): 1-13.
[16]	Saleem M A, Malik T A, Shakeel A, et al.QTL mapping for some important drought tolerant traits in upland cotton[J]. The Journal of Animal & Plant Sciences, 2015, 25(2): 502-509.
[17]	Abdelraheem A, Hughs S E, Jones D C, et al.Genetic analysis and quantitative trait locus mapping of PEG-induced osmotic stress tolerance in cotton[J]. Plant Breeding, 2014, 134(1): 111-120.
[18]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[18]	Li Hesheng.The experiment principle and technique on plant physiology and biochemistry[M]. Beijing: Higher Education Press, 2000.
[19]	Blum A. Breeding crop varieties for stress environments[J]. Critical Reviews in Plant Sciences, 1984(2): 199-238.
[20]	Khan M K R. A step towards cotton genome assembly through construction of high density genetic map from interspecific cross of G. hirsutum × G. tomentosum[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013.
[21]	Van Ooijen J W. JoinMap? 4: Software for the calculation of genetic linkage maps in experimental populations[CP/OL]. (2006-08-01) [2016-02-18].
[22]	Wang S C, Basten C J, Zeng Z B. Windows QTL cartographer V2.5[CP/OL]. (2012-08-01) [2016-02-18]. .
[23]	Stuber C W, Edwards M D, Wendel J F.Molecular marker-facilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits[J]. Crop Science, 1987, 27: 639-648.
[24]	李少昆, 肖璐, 黄文华. 不同时期干旱胁迫对棉花生长和产量的影响Ⅱ.棉花生长发育及生理特性的变化[J]. 石河子大学学报(自然科学版), 1999, 3(4): 259-264.
[24]	Li Shaokun, Xiao Lu, Huang Wenhua, et al.Effect of drought stress on cotton growth and lint yield at different growing stage II. The change of cotton growth and physiological characteristics to water stress[J]. Journal of Shihezi University (Natural Science), 1999, 3(4): 259-264.
[25]	Bowler C, van Montagu M, Inzé D. Superoxide dismutase and stress tolerance[J]. Annual Review of Plant Physiology & Plant Molecular Biology, 1992, 43(1): 83-116.
[26]	刘娥娥, 宗会, 郭振飞, 等. 干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J]. 热带亚热带植物学报, 2000, 8(3): 235-238.
[26]	Liu E E, Zong Hui, Guo Zhenfei, et al.Effects of drought, salt and chilling stresses on accumulation of proline in shoot of rice seedlings[J]. Journal of Tropical and Subtropical Botany, 2000, 8(3): 235-238.
[27]	黄玉山, 罗广华, 关棨文. 镉诱导植物的自由基过氧化损伤[J]. 植物学报, 1997, 39(6): 522-526.
[27]	Huang Yushan, Luo Guanghua, Guan Qiwen, et al.Peroxidation damage of oxygen free radicals induced by cadmium to plant[J]. Acta Botanica Sinica, 1997, 39(6): 522-526.
[28]	吴银明, 王平, 刘洪升, 等. 分根PEG胁迫对羊草幼苗植物量、活性氧代谢及脯氨酸含量的影响[J]. 甘肃农业大学学报, 2008, 43(2): 114-119.
[28]	Wu Yinming, Wang Ping, Liu Hongsheng, et al.Effects of PEG stress on phytomass, active oxygen metabolism and proline content of Leymus chinensis seedlings under split-root condition[J]. Journal of Gansu Agricultural University, 2008, 43(2): 114-119.
[29]	Qian Y L, Wilhelm S J, Marcum K B.Comparative responses of two Kentucky bluegrass cultivars to salinity stress[J]. Crop Science, 2001, 41(6): 1895-1900.
[30]	Zhang Tianzhen, Hu Yan, Jiang Wenkai, et al.Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement[J]. Nature Biotechnology, 2015, 33(4): 531-537.