高温胁迫对Bt棉铃壳Bt蛋白含量及氮代谢影响

doi:10.11963/issn.1002-7807.201601004

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棉花学报 ›› 2016, Vol. 28 ›› Issue (1) : 27-33. DOI: 10.11963/issn.1002-7807.201601004

研究与进展

高温胁迫对Bt棉铃壳Bt蛋白含量及氮代谢影响

衡丽，王俊，Abidallah Eltayib，花明明，张祥，陈源，陈德华^*

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The Effect of High-Temperature Stress on Bt Protein Content and Nitrogen Metabolism in the Boll Shell of Bt Cotton

Heng Li, Wang Jun, Abidallah Eltayib, Hua Mingming, Zhang Xiang, Chen Yuan, Chen Dehua^*

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摘要

为研究生产上出现的高温气候下Bt棉不抗虫现象的原因，本文以两个不同类型Bt棉品种为材料，在人工气候室内设计3、5、7 d的高温（39 ℃）处理，探讨高温对棉铃虫首先取食器官铃壳Bt蛋白含量及氮代谢的影响。结果表明，高温持续胁迫7 d内，铃壳Bt蛋白含量呈持续下降特征，持续胁迫3 d内尤其是在1 d内下降幅度最大。相应地，铃壳中可溶性蛋白含量、谷氨酸丙酮酸转氨酶(GPT)和谷氨酸草酰乙酸转氨酶(GOT)活性下降；铃壳中游离氨基酸含量，蛋白酶和肽酶活性提高，尤其以胁迫1 d内变化幅度最大。因此，铃期高温气候会对Bt棉抗虫性有较大影响，应及时做好棉铃虫等相关害虫的防治。

Abstract

This study explored the reason for observed low insect resistance under high temperatures in Bt cotton production.Two Bt cotton cultivars were selected as experimental materials, and their Bt protein content and the nitrogen metabolic physiology of the boll wall were investigated under the stress of high temperature(39 ℃) growth for 3, 5, and 7 days in an artificial climate chamber. The Bt protein content declined within 7 d of high temperature stress, with the greatest reduction observed within 3 d, especially at 1 d. Correspondingly, the boll wall soluble protein content, and activities of glutamate pyruvate transaminase and glutamate oxaloacetate transaminase reduced, while the free amino acid content, and protease and peptidase activities increased, with the biggest change also detected with in 1 d of the high temperature stress. These results suggest that insect resistance of the boll stage decreases markedly under a high temperature climate, indicating that suitable management strategies should be devised to prevent damage from boll worm and related pests.

导出引用

衡丽, 王俊, Abidallah Eltayib, 花明明, 张祥, 陈源, 陈德华. 高温胁迫对Bt棉铃壳Bt蛋白含量及氮代谢影响[J]. 棉花学报, 2016, 28(1): 27-33. https://doi.org/10.11963/issn.1002-7807.201601004

Heng Li, Wang Jun, Abidallah Eltayib, Hua Mingming, Zhang Xiang, Chen Yuan, Chen Dehua. The Effect of High-Temperature Stress on Bt Protein Content and Nitrogen Metabolism in the Boll Shell of Bt Cotton[J]. Cotton Science, 2016, 28(1): 27-33. https://doi.org/10.11963/issn.1002-7807.201601004

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基金

国家自然科学基金(31171479，31301263，31471435)；教育部博导基金(20113250110001)；江苏省三新工程项目(SXGC(2014)317)；江苏省高等教育优势学科发展计划

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2015-03-10	2016-01-15
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