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棉花学报 ›› 2015, Vol. 27 ›› Issue (2): 159-165.doi: 10.11963/issn.1002-7807.201502009

• 研究与进展 • 上一篇    下一篇

黄萎病菌侵染对感病棉花品种叶片蛋白质组的影响

沈凡瑞,张文蔚,张华崇,刘凯,简桂良*   

  1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193
  • 收稿日期:2014-04-02 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: gljian@ippcaas.cn
  • 作者简介:沈凡瑞(1989―),女,硕士,shenfanrui1989@163.com
  • 基金资助:

    转基因生物新品种培育科技重大专项(2011ZX08005-0041);公益性行业(农业)科研专项(201503109)

Changes in the Proteome of Susceptible Cotton Leaves after Inoculation of Verticillium dahliae

Shen Fanrui, Zhang Wenwei, Zhang Huachong, Liu Kai, Jian Guiliang*   

  1. nstitute of Plant Protection, CAAS/State Key Laboratory for Biology of Plant Diseases and Insect Pest, Beijing 100193, China
  • Received:2014-04-02 Online:2015-03-15 Published:2015-03-15

摘要: 为进一步探索黄萎病菌与棉花的相互作用,以感黄萎病棉花品种冀棉11为实验材料,在接种大丽轮枝菌V991 3 d后提取叶片蛋白,运用双向电泳和质谱分析技术研究黄萎病菌侵染后叶片蛋白质组学的变化。分析发现,与对照相比差异表达量在2倍以上的蛋白点有22个,其中上调表达的蛋白点10个,下调表达的蛋白点12个。质谱鉴定发现,上调表达的蛋白包括ATP合成酶β亚基,RuBisCO活化酶1,RuBisCO活化酶α2以及果糖-1,6-二磷酸醛缩酶,下调表达的蛋白包括质体叶绿素AB结合蛋白,核酮糖二磷酸羧化酶大链以及核酮糖二磷酸羧化酶小链。分别对下调表达的蛋白rubisco小亚基基因(RBCS)和叶绿素AB结合蛋白基因(cab)、上调表达的蛋白RuBisCO活化酶1基因(RCA1)进行荧光定量PCR,发现接种黄萎病菌后3个基因在mRNA水平与蛋白水平上的变化是一致的。通过分析推测,黄萎病菌通过与参与光合能量代谢的蛋白相互作用,破坏植物的光合作用,近而引起棉花叶片的萎蔫与凋零。

关键词: 棉花叶片; 黄萎病菌; 蛋白质组; 实时荧光定量

Abstract: To explore the interaction mechanism between Gossypium hirsutum and Verticillium dahliae, total protein of Jimian 11 leaves inoculated with V. dahliae for 3 days was extracted to analyze proteomic changes. Twenty-two protein spots were found showing a change of more than 2-fold between the inoculated and the mock-inoculated samples, of which 10 were up-regulated and 12 were down-regulated. The up-regulated proteins included subunit beta of ATP synthase, RuBisCO activase 1, RuBisCO activase alpha 2 and fructose-1,6-bisphosphate aldolase. The down-regulated protein spots included chloroplast chlorophyll A-B binding protein, ribulose bisphosphate carboxylase big chain and ribulose bisphosphate carboxylase small chain. Real-time polymerase chain reaction of RBCS, cab and RCA1 genes showed that expression changes of the three genes were consistent at the mRNA and protein levels. From the analyses conducted, we hypothesized that the pathogen might interact with proteins participating in photosynthetic energy metabolism, disrupting cotton photosynthesis and causing wilting and withering of cotton leaves.

Key words: cotton leaves; Verticillium dahliae; proteomics; qRT-PCR

中图分类号: 
  • S435.621