陆地棉主要育种性状SSR关联位点的验证及优异材料鉴定（2022年11月8日更新）

doi:10.11963/cs20210066

摘要/Abstract

摘要：

【目的】评价与陆地棉纤维品质、产量、黄萎病抗性等主要育种性状关联的简单重复序列（simple sequence repeat, SSR）位点的选择效果，并挖掘优异典型材料。【方法】在474份陆地棉中，利用双尾t-检验在26个关联标记中筛选具有显著筛选效果的变异位点，并鉴定典型材料。【结果】共检测到30个多态性位点，基于上述位点可将其中420份陆地棉材料完全区分。20个变异位点与目标性状显著（P<0.05）关联，其中，与铃重和纤维长度密切关联的变异位点各6个，与马克隆值、伸长率、株高和铃数关联的位点分别有3、3、1和1个。获得了与铃重（JESPR220-160+BNL2440-240+BNL226-320）和纤维长度（CM45-140+CGR6528-180+JESPR152-125）密切关联的分子标记组合各1个，表型效应分别达0.27 g和1.27 mm，根据上述标记位点鉴定优异典型材料14份。【结论】获得了具有显著筛选效果的多态性位点及优异典型材料，可为棉花分子标记辅助选择及组合选配提供参考。

关键词: 陆地棉; 表型性状; SSR位点; 筛选效果; 典型材料

Abstract:

[Objective] This study aims to evaluate and validate SSR (simple sequence repeat) markers associated with traits of fiber quality, yield, and resistance to Verticillium wilt of upland cotton, and identify typical materials carrying elite alleles. [Method] Among the 26 SSR markers identified from previous studies, the two-tailed t-test was used to screen the mutation sites with significant effect on phenotypic variations of 474 upland cotton materials, and to identify typical materials. [Result] A total of 30 polymorphic loci were detected, and can be used to fully differentiate 420 upland cotton materials. Interestingly, 20 mutation sites were significantly associated with target traits (P<0.05), of which, 6 mutation sites were closely related to boll weight and fiber length, respectively. Moreover, there are 3, 3, 1, and 1 loci associated with micronaire value, breaking elongation, plant height and boll number, respectively. And, the marker combinations (JESPR220-160+BNL2440-240+BNL226-320) and (CM45-140+CGR6528-180+JESPR152-125) were associated with the boll weight and fiber length, with phenotypic effect of 0.27 g and 1.27 mm, respectively. Furthermore, 14 typical materials carrying elite alleles were obtained. [Conclusions] In this study, polymorphic sites with significant phenotypic effects and typical materials of elite alleles were obtained, which provided references for cotton molecular marker-assisted selection and material selection.

Key words: upland cotton; phenotypic traits; SSR locus; screening effect; typical material

张素君, 李兴河, 王海涛, 唐丽媛, 蔡肖, 刘存敬, 张香云, 张建宏. 陆地棉主要育种性状SSR关联位点的验证及优异材料鉴定（2022年11月8日更新）[J]. 棉花学报, 2022, 34(2): 120-136.

Zhang Sujun, Li Xinghe, Wang Haitao, Tang Liyuan, Cai Xiao, Liu Cunjing, Zhang Xiangyun, Zhang Jianhong. Verification of SSR-associated loci for main breeding traits and identification of excellent germplasms in upland cotton (Gossypium hirsutum L.)[J]. Cotton Science, 2022, 34(2): 120-136.

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链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/cs20210066

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2022/V34/I2/120

图/表 9

表1

表2

表3

表4

表5

具有显著（P<0.05）筛选效果的标记位点信息"

关联性状 Associate traits	P值 P value	关联标记 Associated marker	条带大小Band size/ bp	1带型（1 type band）均值±标准误 Mean±SE	0带型（0 type band）均值±标准误 Mean±SE	表型效应 Phenotypic effect	典型材料（部分） Typical materials (Not all listed)
BW/g	0.01	BNL2651	200	5.43±0.12	5.70±0.03	-0.27	2-20早 2-20 zao，JMX-17，中棉所36 CCRI 36，冀220早熟 Ji 220 zaoshu，新陆早48号 Xinluzao 48
	0.00	JESPR220	150	5.50±0.07	5.72±0.03	-0.21	苏优6109 Suyou 6109，豫389 Yu 389，中棉所58 CCRI 36，中AR-RNX311 Zhong AR-RNX311，中棉所36 CCRI 36
	0.00	JESPR220	160	5.73±0.03	5.38±0.09	0.35	JMX-18，大桃资源 Dataoziyuan，12-6034，04N4，冀3927 Ji 3927;ZXJDL F6
	0.01	BNL2440	240	5.70±0.03	4.43±0.40	1.27	JMX-18，大桃资源 Dataoziyuan，12-6034，冀3927 Ji 3927，鲁棉研28 Lumianyan 28
	0.02	BNL226	205	5.11±0.18	5.69±0.03	-0.58	新陆早58号 Xinluzao 58，SJB092，新陆早60号 Xinluzao 60，新陆早48号 Xinluzao 48
	0.01	BNL226	320	5.72±0.03	5.48±0.12	0.25	JMX-18，大桃资源 Dataoziyuan，12-6034，科林9828 Kelin 9828，冀3927 Ji 3927;ZXJDL F6
	0.00	JESPR220-160 +BNL2440-240 +BNL226-320		5.77±0.03	5.50±0.05	0.27	JMX-18，大桃资源 Dataoziyuan，12-6034，冀3927 Ji 3927，12-6032;ZXJDL F6
FUHML/mm	0.04	CM45	130	28.37±0.10	28.62±0.10	-0.26	抗虫 Kangchong，湘杂棉 Xiangzamian，润棉1号 Runmian 1，冀466-468 Ji 466-468，冀棉红叶 Jimianhongye，YS58
	0.00	CM45	140	28.68±0.10	28.23±0.10	0.45	2011NWF，冀棉2016 Jimian 2016，JMX-39，金陆长1号 Jinluchang 1，资优 Ziyou
	0.03	CGR6528	180	28.58±0.11	28.31±0.10	0.28	2011NWF，冀棉2016 Jimian 2016，中S9612-1 Zhong S9612-1，特长绒 Techangrong，Q1087
	0.00	CGR6528	185	28.26±0.09	28.65±0.13	-0.39	抗虫 Kangchong，湘杂棉 Xiangzamian，12YC9-06，柳叶棉 Liuyemian，冀466-468 Ji 466-468，05N322
	0.00	JESPR152	125	29.00±0.18	28.40±0.08	0.60	2011NWF，冀棉2016 Jimian 2016，金陆长1号 Jinluchang 1，新陆早66号 Xinluzao 66，新陆中36号 Xinluzhong 36
FUHML/mm	0.03	NAU4057	140	28.32±0.09	28.63±0.13	-0.30	柳叶棉 Liuyemian，冀466-468 Ji 466-468，脱字棉 Trice cotton，2-20早 2-20zao，润棉1号 Runmian 1
	0.00	CM45-140 +CGR6528-180 +JESPR152-125		29.76±0.38	28.48±0.07	1.27	2011NWF，冀棉2016 Jimian 2016，新陆早66号 Xinluzao 66，新陆中36号 Xinluzhong 36，冀棉128 Jimian 128
MV	0.04	DPL0886	155	5.29±0.03	5.21±0.03	0.08	脱字棉 Trice cotton，1535系 1535 xi，邯103 Han 103，567系 567 Xi，科欣7号 Kexin 7
	0.04	DPL0886	158	5.21±0.03	5.29±0.03	-0.08	绿绒 Lürong，冀棉2016 Jimian 2016，2011NWF，GH25，新陆中11号 Xinluzhong 11
	0.00	NAU3607	190	5.35±0.04	5.20±0.02	0.15	JMX-41，1535系 1535 xi，邯103 Han 103，567系 567 xi，科欣7号 Kexin 7
FE/%	0.00	NAU3607	150	6.81±0.03	6.57±0.05	0.24	2011NWM，光子资源 Guangziziyuan，JMX-8，新陆中7号 Xinluzhong 7，鲁棉研27 Lumianyan 27
	0.00	HAU1774	320	6.79±0.02	6.59±0.06	0.20	2011NWM，光子资源 Guangziziyuan，Z311-2，新陆中7号 Xinluzhong 7，鲁棉研27 Lumianyan 27
	0.00	BNL1151	210	6.92±0.05	6.77±0.02	0.15	JMX-9，长绒 Changrong，新陆中14号 Xinluzhong 14，新陆中7号 Xinluzhong 7，鲁棉研27 Lumianyan 27
PH/cm	0.02	BNL2448	170	95.04±0.41	90.63±2.47	4.41	石抗163 Shikang 163，冀棉128 Jimian 128，JMX-28，木胞 Mubao，冀棉2016 Jimian 2016
BN	0.02	BNL1646	420	15.39±0.20	16.45±0.49	-1.05	中棉所58 CCRI 58，中棉所64号 CCRI 64，新陆早7号 Xinluzao 7 ，辽棉7号 Liaomian 7，中棉所26 CCRI 26

表5

图1

表6

附表1

474份陆地棉材料"

编号 No.	材料 Materials	聚类亚群 Subgroup	编号 No.	材料 Materials	聚类亚群 Subgroup
1	冀丰107 Jifeng 107	I	2	鲁16188Bt Lu16188Bt	I
3	JMX-1	I	4	美8123 Mei 8123	I
5	JMX-2	I	6	M-8124-1159	II
7	JMX-3	I	8	中资3012 Zhongzi 3012	I
9	中棉所16 ICR 16	I	10	徐州142 Xuzhou 142	I
11	中棉所49 ICR 49	II	12	JMX-4	II
13	A41772152	I	14	JMX-5	II
15	超早3号 Chaozao 3	I	16	JMX-6	II
17	A971Bt	I	18	中AR-RNX311 Zhong AR-RNX311	I
19	中Arc-315 Zhong Arc-315	II	20	莎24-3 Sha 24-3	II
21	JMX-7	I	22	湘212 Xiang 212	II
23	苏棉12 Sumian 12	I	24	JMX-8	I
25	苏优6109 Suyou 6109	I	26	邯682 Han 682	I
27	邯郸305 Handan 305	I	28	邯103 Han 103	I
29	邯棉102 Hanmian 102	I	30	邱县0905 Qiuxian 0905	I
31	中棉所64 ICR 64	I	32	中棉所58 ICR 58	I
33	鄂农071 Enong 071	I	34	中0102X-10-1 Zhong 0102X-10-1	I
35	银99 Yin 99	I	36	JMX-9	II
37	中棉所36-1 ICR 36-1	I	38	中棉所50 ICR 50	I
39	JMX-10	I	40	新陆早2号 Xinluzao 2	I
41	JMX-11	II	42	JMX-12	I
43	JMX-13	I	44	新陆早16号 Xinluzao 16	I
45	新陆中1号 Xinluzhong 1	I	46	新陆中7号 Xinluzhong 7	I
47	JMX-14	I	48	新陆中14号 Xinluzhong 14	I
49	JMX-15	I	50	新陆中17号 Xinluzhong 17	I
51	JMX-16	II	52	新陆中36号 Xinluzhong 36	II
53	JMX-17	I	54	JMX-18	I
55	科林9828 Kelin 9828	I	56	鲁棉研41 Lumianyan 41	II
57	鲁垦棉33 Lukenmian 33	II	58	渝棉1号 Yumian 1	I
59	SGK958	II	60	中棉所79 ICR 79	II
61	银山7号 Yinshan 7	I	62	JMX-19	I
63	JMX-20	I	64	新陆中44 Xinluzhong 44	I
65	银瑞361 Yinrui 361	I	66	JMX-21	I
67	华棉5号 Huamian 5	I	68	JMX-22	I
69	卞棉5号 Bianmian 5	I	70	新陆中35 Xinluzhong 35	I
71	鲁棉研27 Lumianyan 27	I	72	仁和39号 Renhe 39	I
73	锦科棉9号 Jinkemian 9	I	74	鲁棉研36 Lumianyan 36	I
75	JMX-23	I	76	山东棉8号 Shandongmian 8	I
77	JMX-24	I	78	sgk9708	I
79	2012N-WSJ-4	I	80	李资 Lizi	I
81	JMX-25	I	82	GH25	I
83	JMX-26	I	84	JMX-27	I
85	光子资源 Guangziziyuan	I	86	鲁HB红花 Lu HB honghua	I
87	JMX-29	I	88	冀棉169 Jimian 169	I
89	JMX-30	I	90	冀3927 Ji 3927	I
91	JMX-32	I	92	JMX-33	I
93	JMX-34	II	94	长绒 Changrong	I
95	12-6032	I	96	12-6034	I
97	12-6035	I	98	1535系 1535 xi	I
99	JMX-35	I	100	衡无89-30 Hengwu 89-30	I
101	中9806 Zhong 9806	I	102	JMX-36	I
103	中S9612-1 Zhong S9612-1	I	104	芽黄 Yahuang	I
105	邯无198 Hanwu 198	I	106	红叶 Hongye	I
107	Kangg	I	108	JMX-37	I
109	JMX-38	I	110	1498系 1498 xi	I
111	Z311-2	I	112	鲁棉研28 Lumianyan 28	I
113	中棉所45-1 ICR 45-1	I	114	2-20早 2-20 zao	I
115	2011NWF	I	116	JMX-39	I
117	2011N西北g-1 2011N xibeig-1	I	118	JMX-40	II
119	567系 567 xi	I	120	Z10	I
121	04N4	I	122	N71	I
123	JMX-41	I	124	石抗538 Shikang 538	I
125	中1010 Zhong 1010	I	126	鲁棉338 Lumian 338	I
127	WH	I	128	冀棉262 Jimian 262	I
129	JMX-42	II	130	GB996	I
131	绿绒 Lvrong	I	132	棕绒 Zongrong	I
133	CCSL-60	I	134	红花系 Honghuaxi	I
135	芝麻棉 Zhimamian	I	136	对桃资源 Duitaoziyuan	II
137	楚优8号 Chuyou 8	I	138	资优 Ziyou	I
139	2011NWM	I	140	冀棉128 Jimian 128	I
141	冀棉2016 Jimian 2016	I	142	03-73	II
143	03-466-468	I	144	4133Bt	I
145	YC 14-5	I	146	12YC9-06	I
147	12-SZ1-02	I	148	05N322	I
149	Z311-2×1498	I	150	JMX-45	I
151	2011光子-2 2011 guangzi -2	I	152	379白花 379 baihua	I
153	379浅黄色 379 qianhuangse	I	154	G190	I
155	JMX-28	I	156	红花 Honghua	I
157	远1闭花 Yuan 1 bihua	I	158	JMX-46	I
159	马里4号 Mali 4	I	160	JMX-47	I
161	JMX-48	I	162	TAM91D-3	I
163	JMX-49	I	164	斯字棉5A Sizimian 5A	I
165	脱字棉 Tuozimian	I	166	岱字棉16 Daizimian 16	I
167	TM-1	I	168	岱红岱 Daihongdai	I
169	99B	I	170	乌1 Wu 1	I
171	乌3 Wu 3	I	172	Acala 3080	I
173	Arkot 8717	I	174	7235	I
175	奥棉618 Aomian 618	I	176	朝鲜2号 Chaoxian2 号	I
177	G.mexicanum-5	I	178	LA887宿Bt LA887 su Bt	I
179	冀棉红叶 Jimianhongye	I	180	新陆早24号 Xinluzao 24	I
181	JMX-50	I	182	新路早24-13 Xinluzao 24-13	I
183	中棉所36-2 ICR 36-2	I	184	中棉所45-2 ICR 45-2	I
185	126-9	I	186	177系 177 xi	I
187	69307	I	188	JC	I
189	75195	I	190	郑204 Zheng 204	I
191	LfA80319	I	192	177JC	I
193	澳L23757 Ao L23757	I	194	柳棉2号 Liumian 2	I
195	柳叶棉 Liuyemian	I	196	库车T94-6 Kuche T94-6	I
197	鄂农071 Enong 071	I	198	晋棉35号 Jinmian 35	I
199	中078 Zhong 078	I	200	2358B-4	I
201	PZ36	II	202	PZ32	I
203	PZ38	II	204	新陆早1号 Xinluzao 1	I
205	新陆早5号 Xinluzao 5	I	206	新陆早6号 Xinluzao 6	I
207	JMX-51	I	208	新陆早7号 Xinluzao 7	II
209	新陆早8号 Xinluzao 8	I	210	新陆早9号 Xinluzao 9	I
211	新陆早11号 Xinluzao 11	I	212	新陆早12号 Xinluzao 12	I
213	新陆早13号 Xinluzao 13	I	214	新陆早38号 Xinluzao 38	II
215	新陆早17号 Xinluzao 17	I	216	新陆早18号 Xinluzao 18	I
217	新陆早26号 Xinluzao 26	I	218	新陆早29号 Xinluzao 29	I
219	新陆早36号 Xinluzao 36	I	220	新陆早48号 Xinluzao 48	I
221	新陆早58号 Xinluzao 58	I	222	新陆早60号 Xinluzao 60	I
223	新陆早62号 Xinluzao 62	I	224	新陆早64号 Xinluzao 64	I
225	新陆中10号 Xinluzhong 10	I	226	新陆中11号 Xinluzhong 11	I
227	新陆中16号 Xinluzhong 16	I	228	新陆中18号 Xinluzhong 18	I
229	新陆中37号 Xinluzhong 37	I	230	新陆中44号 Xinluzhong 44	I
231	新陆中6号 Xinluzhong 6	II	232	新陆中8号 Xinluzhong 8	I
233	新陆中9号 Xinluzhong 9	I	234	新陆中21号 Xinluzhong 21	I
235	新陆中35号 Xinluzhong 35	I	236	新路早66号 Xinluzhong 66	II
237	Bp-1	I	238	Bp-2	I
239	Bp-4	II	240	CTC14-3	I
241	CHK	I	242	CCJ	I
243	JXH1	I	244	冀棉抗 Jimiankang	II
245	储抗1 Chukang 1	I	246	Hn9311-1-04	I
247	L399	I	248	JIAK	I
249	3H-52	I	250	XGX7	I
251	L390-1	I	252	国丰棉5号 Guofengmian 5	I
253	航棉16 Hangmian 16	I	254	川109 Chuan 109	I
255	川2783 Chuan 2783	I	256	川87-14 Chuan 87-14	I
257	川高衣分58 Chuangaoyifen 58	I	258	金陆长1号 Jinluchang 1	I
259	F9999	I	260	M9999	I
261	鄂0901 E 0901	I	262	鄂0903 E 0903	I
263	荆楚43 Jingchu 43	I	264	JA060	I
265	JA064	I	266	JA072	I
267	新抗4 Xinkang 4	I	268	SJB092	I
269	SJB094	I	270	SJB095	I
271	JA412	I	272	JA501	II
273	JA532	I	274	冀棉11 Jimian 11	II
275	JA542	I	276	J02-508	I
277	鄂棉9号 Emian 9	I	278	AS11	I
279	CD-121	I	280	HS286	I
281	KB7038	I	282	NTY-1	I
283	LMX-52	I	284	V4054	I
285	抗1 Kang 1	I	286	V4015	I
287	V4055	I	288	F0145	I
289	V4050	I	290	F0518	I
291	V4008	I	292	601长绒棉 601 Changrongmian	I
293	X6028	I	294	冈4028 Gang 4028	I
295	谷神棉1号 Gushenmian 1	I	296	中609-2 Zhong 609-2	I
297	华惠17 Huahui 17	I	298	荆3180 Jing 3180	I
299	荆棉107 Jingmian 107	I	300	晶华115 Jinghua 115	I
301	科欣7号 Kexin 7	I	302	宜棉3号 Yimian 3	I
303	GB119	I	304	湘杂棉 Xiangzamian	I
305	湘16 Xiang 16	I	306	湘抗85-1 Xiangkang 85-1	I
307	gh2	I	308	冀丰908 Jifeng 908	I
309	冀棉1号 Jimian 1	I	310	冀省03H17 Jisheng03H17	I
311	冀省04H113 Jisheng04H113	I	312	冀省远1闭花 Jishengyuan1 bihua	I
313	木胞 Mubao	III	314	冀无252 Jiwu 252	I
315	邱县0904 Qiuxian 0904	I	316	CCSL-2	I
317	丰抗棉1号 Fengkangmian 1	I	318	大桃资源 Datiaoziyuan	I
319	冀298 Ji 298	I	320	创优棉9号 Chuangyoumian 9	I
321	冀04N4 Ji 04N4	I	322	冀1096系 Ji 1096 xi	I
323	冀1467 Ji 1467	I	324	冀1498系 Ji1498xi	II
325	冀220早熟 Ji220zaoshu	I	326	JMX-31	I
327	冀466-468 Ji 466-468	I	328	冀567系 Ji 567xi	I
329	冀N71 JiN71	I	330	冀NJ3 JiNJ3	I
331	冀NWF Ji NWF	I	332	冀NWm Ji NWm	I
333	冀Z10 JiZ 10	I	334	冀Z8号 JiZ 8	I
335	冀长绒 Jichangrong	I	336	冀东捡 Jidongjian	I
337	冀丰103 Jifeng 103	I	338	冀丰1271 Jifeng 1271	I
339	冀丰4号 Jifeng 4	I	340	冀棉333 Jimian 333	I
341	中609-1 Zhong 609-1	I	342	冀棉616 Jimian 616	I
343	冀光子 Jiguangzi	I	344	冀资优 Jiziyou	I
345	快育66 Kuaiyu 66	I	346	宿9108 Su 9108	I
347	邯棉3006 Hanmian 3006	I	348	邯686 Han 686	I
349	邯3007 Han 3007	I	350	14214621	I
351	石抗368 Shikang 368	I	352	石抗163 Shikang 163	I
353	石早2号 Shizao 2	I	354	石早3号 Shizao 3	I
355	冀HN1204 JiHN1204	I	356	190系 190 xi	I
357	393系 393 xi	I	358	旱农1号 Hannong 1	I
359	1142选 1142 Xuan	I	360	冀HN1209 JiHN1209	I
361	Hn4307	I	362	苏51005 Su51005	I
363	苏徐138 Suxu 138	II	364	泗阳6821 Siyang 6821	I
365	泗棉3号 Simian 3	I	366	苏优6036 Suyou 6036	I
367	701	I	368	A1684	I
369	盐农棉4号 Yannongmian 4	I	370	苏9934 Su9934	I
371	苏研65006 Suyan 65006	I	372	苏研65010 Suyan 65010	I
373	苏研65017 Suyan 65017	I	374	苏研65057 Suyan 65057	I
375	苏研65068 Suyan 65068	I	376	JMX-53	I
377	科棉5号 Kemian 5	I	378	辽08405 Liao 08405	II
379	辽棉7号 Liaomian 7	I	380	辽棉9号 Liaomian 9	I
381	关农1号 Guannong 1	I	382	黑山棉1号 Heishanmian 1	I
383	定陶621 Dingtao 621	II	384	鑫秋1号 Xinqiu 1	I
385	华棉08-3109 Huamian 08-3109	I	386	润棉1号 Runmian 1	I
387	冠棉4号 Guanmian 4	I	388	银兴棉4号 Yinxingmian 4	I
389	高密933 Gaomi 933	I	390	临清1526 Linqing 1526	I
391	特长绒 Techangrong	I	392	鲁6269 Lu 6269	I
393	鲁棉研29号 Lumianyan 29	I	394	鲁棉研37号 Lumianyan 37	I
395	5029	I	396	YS58	I
397	CQ1510	I	398	CQ1522	I
399	JMX-54	I	400	CQ1530	I
401	CQ1531	I	402	CQ1532	I
403	鲁棉353 Lumian 353	I	404	鲁棉312231 Lumian 312231	I
405	P19	I	406	RP10-4	I
407	SD127	I	408	SSCL-Chr.7	I
409	鲁棉研22号 Lumianyan 22	I	410	鲁735 Lu 735	I
411	鲁7619 Lu 7619	I	412	2013-A3	I
413	2013-B2	I	414	2013-B3	I
415	2013-B6	I	416	晋棉38 Jimian 38	I
417	晋棉34 Jinmian 34	I	418	陕204 Shan 204	I
419	新木棉5号 Xinmumian 5	I	420	锦科2号 Jinke 2	I
421	银山6号 Yinshan 6	I	422	JMX-55	I
423	欣抗4号 Xinkang 4	I	424	郑306 Zheng 306	I
425	郑310 Zheng 310	I	426	绿棉2号 Lvmian 2	I
427	9917	I	428	F20098	I
429	豫棉21号 Yumian 21	I	430	卞棉5号 Bianmian 5	I
431	郑农棉4号 Zhengnongmian 4	I	432	豫389 Yu 389	I
433	中129 Zhong 129	I	434	中507145 Zhong 507145	I
435	中H177 Zhong H177	I	436	MBC31176	I
437	石华1号 Shihua 1	I	438	NOCH8	I
439	B9	I	440	GK219	I
441	B8	I	442	7015系 7015 xi	I
443	MBC30181	I	444	MBC11181	I
445	MBC31081	I	446	MB10004	I
447	NC-2	I	448	MBC11761-049	I
449	MBC31068	I	450	MB9302-03	I
451	Q1087	I	452	75119	I
453	MSC9743	I	454	MBC19488	I
455	MBC27683	I	456	MBC11888	I
457	Y1-0-153-7235	I	458	s641	I
459	MBI5900-15	I	460	中R014121 ZhongR014121	I
461	LMJ1	I	462	JMX-56	II
463	中棉所17号 ICR 17	I	464	中棉所69号 ICR 69	I
465	中棉所58号 ICR 58	I	466	中棉所64号 ICR 64	I
467	中棉所15 号 ICR 15	I	468	中棉所26 ICR 26	I
469	Ari3697	I	470	中远911 Zhongyuan 911	I
471	lfg2009	I	472	lfg7006	I
473	10008	I	474	JMX-57	I

附表1

附表2

SSR标记信息"

标记	正向引物	反向引物	等位变异数	基因多样性指数	多态性信息含量
Marker	Forward primer	Reverse primer	Number of alleles	Gene diversity	PIC
BNL2448	TAACTTTATCCGCGGAATCG	TAGTTTGATCAGTTTGATGATTTAGC	2	0.065 6	0.063 4
BNL2651	CGAGTGTCTCCAAAACATCG	AGATGATGAGGGGGAGGTTT	2	0.107 2	0.101 5
BNL2632-2	CGTGTCTCCAGACCAACAAA	GGGAGTTGAAGCCGACATAA	2	0.153 1	0.141 4
NAU943	ATCTGTTCAATTTCTCGTCA	CAGTTGTTGGTTGATCTGGA	4	0.207 8	0.199 6
BNL2632-1	CGTGTCTCCAGACCAACAAA	GGGAGTTGAAGCCGACATAA	2	0.225 3	0.199 9
JESPR220	CGAGGAAGAAATGAGGTTGG	CTAAGAACCAACATGTGAGACC	5	0.226 6	0.202 4
NAU2697	ATTTCCCATGGTCATAGCAG	GAAAGGAGTCGGAAATGAGA	2	0.232 3	0.205 3
MUCS162	GGTTTCTCCGAAAAGAACTCG	TCTCATCGTTTTTCAAAGCC	2	0.234 4	0.206 9
BNL1646-2	TTAAAGGGCAACAAAAGTTCAA	CATGTGATGTAACCTCTCTCTCTCTC	2	0.248 1	0.217 3
NAU5387-2	TTGGTCTTGCTCTCTTCCTC	TCGTGTGAATGAAGCCTAAA	4	0.270 3	0.240 4
BNL1151	AAAGTAGCAGCGGTTCCAAA	GAGCCGCTTCTGTAGCTTCA	5	0.359 8	0.328 5
MUSS162	TTGGTTGGTTAATTACGGGG	GGCTTGTATCTCCCAGCAAG	2	0.377 1	0.306 0
BNL1646-1	TTAAAGGGCAACAAAAGTTCAA	CATGTGATGTAACCTCTCTCTCTCTC	2	0.380 1	0.307 8
BNL2646	CCCCTTTGATAGATACACATTTTTA	AAAATAAACTACGAAAGAGAAAGAGAA	2	0.402 0	0.321 2
JESPR152	GATGCACCAGATCCTTTTATTAG	GGTACATCGGAATCACAGTG	5	0.472 5	0.438 5
DPL0492	ATGTTACTGCCATGGTTGGTG	CCATTTGCCATAACGTGTATAGTG	5	0.479 9	0.367 9
CM45	GATGCCAGTAAGTTCAGGAATG	GCCAACTTATATTCGGTTCCT	4	0.480 9	0.367 4
BNL1694	CGTTTGTTTTCGTGTAACAGG	TGGTGGATTCACATCCAAAG	2	0.490 4	0.370 1
DPL0886	GGTTCCAGAGATGACGTGGT	GGTTCAACTCGGTTCGTCAA	2	0.495 3	0.372 6
BNL2440	TGTTAAGCATACATTAGTTTCACTCG	CCGGCACCACAAAAGTAAAT	5	0.495 3	0.387 3
HAU1951	CAAACGCTGTCACTACCAAG	TGACAAAATGTGCTTCCATC	4	0.501 7	0.378 0
CGR6528	TGCAGAAGGCACTAAGAGCA	GCAGAAGTGAAGAGACAAGCG	4	0.512 0	0.393 2
BNL3442	CATTAGCGGATTTGTCGTGA	AACGAACAAAGCAAAGCGAT	4	0.535 5	0.430 1
BNL226	TTATTCTCACAGCCGGAACC	TTCACCCTCTCGCTTCTCAT	5	0.578 6	0.488 5
BNL193	TGTGAGCCATTGCTGTTAGC	TAAGTGCTGGCATTGTGAGC	4	0.587 5	0.500 5
NAU3607	GATCGACCAAACACAGACAA	TTCATCATCATCATCGTCGT	4	0.628 1	0.557 7
HAU1774	CAAATGATTCCTATGGCACA	GAGGGCTTCAAAGGCTAATA	3	0.653 7	0.580 1
NAU5387-1	TTGGTCTTGCTCTCTTCCTC	TCGTGTGAATGAAGCCTAAA	6	0.665 6	0.604 8
NAU4057	ACAACCACCAACGCTAATCT	TCCCTCGTTGTCTTTCTCTC	7	0.728 5	0.684 4
Mean			3.517 2	0.406 7	0.343 5

附表2

参考文献 52

[1]	卢秀茹, 贾肖月, 牛佳慧. 中国棉花产业发展现状及展望[J/OL]. 中国农业科学, 2018, 51(1): 26-36[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2018.01.003.
	Lu Xiuru, Jia Xiaoyue, Niu Jiahui. The present situation and prospects of cotton industry development in China[J/OL]. Scientia Agricultura Sinica, 2018, 51(1): 26-36[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2018.01.003.
[2]	邓琳, 余小刚, 姜朵, 等. 棉花分子育种研究进展[J/OL]. 山东农业科学, 2017, 49(5): 144-150[2021-11-01]. https://doi.org/10.14083/j.issn.1001-4942.2017.05.031.
	Deng Lin, Yu Xiaogang, Jiang Duo, et al. Research progress of cotton molecular breeding[J/OL]. Shandong Agricultural Sciences, 2017, 49(5): 144-150[2021-11-01]. https://doi.org/10.14083/j.issn.1001-4942.2017.05.031.
[3]	Hu Y, Chen J D, Fang L, et al. Gossypium barbadense and Gossypium hirsutum genomes provide insights into the origin and evolution of allotetraploid cotton[J/OL]. Nature Genetics, 2019, 51(4): 739-748[2021-11-01]. https://doi.org/10.1038/s41588-019-0371-5.
[4]	Feng L, Zhou C, Su Q, et al. Fine-mapping and candidate gene analysis of qFS-Chr. D02, a QTL for fibre strength introgressed from a semi-wild cotton into Gossypium hirsutum[J/OL]. Plant Science, 2020, 297(8): 110524[2021-11-01]. https://doi.org/10.1016/j.plantsci.2020.110524.
[5]	Wang P P, He S, Sun G F, et al. Favorable pleiotropic loci for fiber yield and quality in upland cotton (Gossypium hirsutum)[J/OL]. Scientific Reports, 2021, 11(1): 15935[2021-11-01]. https://doi.org/10.1038/S41598-021-95629-9.
[6]	郭志军, 赵云雷, 陈伟, 等. 陆海高代回交群体抗黄萎病QTL定位[J/OL]. 棉花学报, 2019, 31(4): 327-334[2021-11-01]. https://doi.org/10.11963/1002-7807.gzjwhm.20190618.
	Guo Zhijun, Zhao Yunlei, Chen Wei, et al. Mapping of Verticillium wilt resistance QTL with interspecific advanced backcross population between upland cotton (Gossypium hirsutum L.) and sea-island cotton(G. barbadense L.)[J/OL]. Cotton Science, 2019, 31(4): 327-334[2021-11-01]. https://doi.org/10.11963/1002-7807.gzjwhm.20190618.
[7]	Rashid M H, Li P T, Chen T T, et al. Genome-wide quantitative trait loci mapping on Verticillium wilt resistance in 300 chromosome segment substitution lines from Gossypium hirsutum × Gossypium barbadense[J/OL]. G3: Genes Genomes Genetics, 2021, 11(5):jkab027[2021-11-01]. https://doi.org/10.1093/G3JOURNAL/JKAB027.
[8]	商海红, 于霁雯, 石玉真, 等. 棉花优异品质及抗性基因挖掘与分子育种[J/OL]. 棉花学报, 2017, 29(S1): 62-71[2021-11-01]. https://doi.org/10.11963/1002-7807.shhyyl.20170825.
	Shang Haihong, Yu Jiwen, Shi Yuzhen. et al. Identification of QTL/genes of fiber yield, quality and disease resistance, and molecular breeding in cotton[J/OL]. Cotton Science, 2017, 29(S1): 62-71[2021-11-01]. https://doi.org/10.11963/1002-7807.shhyyl.20170825.
[9]	Wang M J, Tu L L, Yuan D J, et al. Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense[J/OL]. Nature Genetics, 2019, 51(2): 224-229[2021-11-01]. https://doi.org/10.1038/s41588-018-0282-x.
[10]	Muhammad J, Jia F, Gong J W, et al. Identification of stable quantitative trait loci (QTL) for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population[J/OL]. BMC Genomics, 2016, 17(1): 197[2021-11-01]. https://doi.org/10.1186/s12864-016-2560-2.
[11]	Gu Q S, Ke H H, Liu Z W, et al. A high-density genetic map and multiple environmental tests reveal novel quantitative trait loci and candidate genes for fibre quality and yield in cotton[J/OL]. Theoretical and Applied Genetics, 2020, 133(12): 1-14[2021-11-01]. https://doi.org/10.1007/s00122-020-03676-z.
[12]	Li H G, Pan Z E, He S P, et al. QTL mapping of agronomic and economic traits for four F₂ populations of upland cotton[J/OL]. Journal of Cotton Research, 2021, 4: 3[2021-11-01]. https://doi.org/10.1186/S42397-020-00076-Y.
[13]	Huang C, Shen C, Wen T W, et al. Genome-wide association mapping for agronomic traits in an 8-way upland cotton MAGIC population by SLAF-seq[J/OL]. Theoretical and Applied genetics, 2021, 134(9): 1-10[2021-11-01]. https://doi.org/10.1007/S00122-021-03835-W.
[14]	Wang H T, Jia X Y, Kang M, et al. QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton[J/OL]. Euphytica, 2021, 217: 102[2021-11-01]. https://doi.org/10.1007/S10681-021-02823-X.
[15]	Wang P, Ning Z Y, Lin L, et al. Genetic dissection of tetraploid cotton resistant to Verticillium wilt using interspecific chromosome segment introgression lines[J/OL]. The Crop Journal, 2014, 2(5): 278-288[2021-11-01]. https://doi.org/10.1016/j.cj.2014.06.007.
[16]	Ning Z Y, Zhao R, Chen H, et al. Molecular tagging of a major quantitative trait locus for broad-spectrum resistance to Verticillium wilt in upland cotton cultivar Prema[J/OL]. Crop Science, 2013, 53(6): 2304-2312[2021-11-01]. https://doi.org/10.2135/cropsci2012.12.0694.
[17]	Palanga K K, Jamshed M, Rashid M H O, et al. Quantitative trait locus mapping for Verticillium wilt resistance in an upland cotton recombinant inbred line using SNP-based high density genetic map[J/OL]. Frontiers in Plant Science, 2017, 8: 382[2021-11-01]. https://doi.org/10.3389/fpls.2017.00382.
[18]	牛豆豆, 李鹏涛, 肖向辉, 等. 陆海渐渗系纤维强度主效QTL分子标记辅助选择效应分析[J/OL]. 分子植物育种2021: 1-17[2021-11-01]. .
	Niu Doudou, Li Pengtao, Xiao Xianghui, et al. Analysis of molecular marker-assisted selection effect of major QTL for fiber strength in introgression lines from Gossypium hirsutum × G. barbadense.[J/OL]. Molecular Plant Breeding, 2021: 1-17[2021-11-01]. .
[19]	Zhang S J, Jiang Z X, Chen J, et al. The cellulose synthase (CesA) gene family in four Gossypium species: phylogenetics, sequence variation and gene expression in relation to fiber quality in upland cotton[J/OL]. Molecular Genetics and Genomics, 2021, 296(2): 355-368[2021-11-01]. https://doi.org/10.1007/S00438-020-01758-7.
[20]	Cui J J, Peng J Z, Cheng J W, et al. Development and validation of genome-wide InDel markers with high levels of polymorphism in bitter gourd (Momordica charantia)[J/OL]. BMC Genomics, 2021, 22(1): 190[2021-11-01]. https://doi.org/10.1186/S12864-021-07499-0.
[21]	张红, 徐莹莉, 王超楠, 等. 大白菜抗干烧心病分子标记的开发与验证[J/OL]. 华北农学报, 2021, 36(6): 195-203[2021-11-01]. https://doi.org/10.7668/hbnxb.20192409.
	Zhang Hong, Xu Yingli, Wang Chaonan, et al. Development and validation of molecular markers for resistance to dry burning heart disease in Chinese cabbage[J/OL]. Acta Agriculturae Boreali-Sinica, 2021, 36(6): 195-203[2021-11-01]. https://doi.org/10.7668/hbnxb.20192409.
[22]	张颖, 赵国龙, 林春晶, 等. 大豆核不育基因ms6功能性分子标记的开发与验证[J/OL]. 植物遗传资源学报, 2021, 22(3): 807-814[2021-11-01]. https://doi.org10.13430/j.cnki.jpgr.20201023001.
	Zhang Ying, Zhao Guolong, Lin Chunjing, et al. Development and validation of functional molecular markers of nuclear sterility gene ms6 in soybean[J/OL]. Journal of Plant Genetic Resources, 2021, 22(3): 807-814[2021-11-01]. https://doi.org10.13430/j.cnki.jpgr.20201023001.
[23]	陈豆豆, 关利平, 贺亮亮, 等. 葡萄无核基因分子标记的通用性鉴定[J/OL]. 中国农业科学, 2021, 54(22): 4880-4895[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2021.22.014.
	Chen Doudou, Guan Liping, He Liangliang, et al. Commonality identification of molecular markers linked to seedless genes in grape[J/OL]. Scientia Agricultura Sinica, 2021, 54(22): 4880-4895[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2021.22.014.
[24]	吴晓军, 胡喜贵, 陈向东, 等. 基于小麦外引种质资源的抗旱性分子标记检测及实用性评价[J/OL]. 华北农学报, 2021, 36(3): 74-82[2021-11-01]. https://doi.org/10.7668/hbnxb.20191845.
	Wu Xiaojun, Hu Xigui, Chen Xiangdong, et al. Detection and practical evaluation of molecular markers associated with drought resistance based on introduced wheat germplasm resources[J/OL]. Acta Agriculturae Boreali-Sinica, 2021, 36(3): 74-82[2021-11-01]. https://doi.org/10.7668/hbnxb.20191845.
[25]	姚姝, 张亚东, 朱镇, 等. 8个水稻抗条纹叶枯病分子标记有效性的验证及评价[J/OL]. 华北农学报, 2012, 27(6): 146-151[2021-11-01]. https://doi.org/10.3969/j.issn.1000-7091.2012.06.029.
	Yao Shu, Zhang Yadong, Zhu Zhen, et al. Evaluation and validation of eight molecular markers associated with rice stripe resistance in rice(Oryza sativa)[J/OL]. Acta Agriculturae Boreali-Sinica, 2012, 27(6): 146-151[2021-11-01]. https://doi.org/10.3969/j.issn.1000-7091.2012.06.029.
[26]	任海红, 刘学义, 朱保葛, 等. 大豆油分相关分子标记的实用性分析与验证[J/OL]. 作物杂志, 2015, 31(5): 37-40[2021-11-01]. https://doi.org/10.16035/j.issn.1001-7283.2015.05.007.
	Ren Haihong, Liu Xueyi, Zhu Baoge, et al. Practical analysis and verification of molecular marker of oil in soybean[J/OL]. Crops, 2015, 31(5): 37-40[2021-11-01]. https://doi.org/10.16035/j.issn.1001-7283.2015.05.007.
[27]	刘春晓, 兰进好, 侯鸿敏, 等. 苹果抗炭疽菌叶枯病基因相关的4个分子标记的准确性验证[J/OL]. 园艺学报, 2017, 44(7): 1355-1362[2021-11-01]. https://doi.org/10.16420/j.issn.0513-353x.2017-0047.
	Liu Chunxiao, Lan Jinhao, Hou Hongmin, et al. Accuracy test of four molecular markers of glomerella leaf spot resistant gene in apple cultivars[J/OL]. Acta Horticulturae Sinica, 2017, 44(7): 1355-1362[2021-11-01]. https://doi.org/10.16420/j.issn.0513-353x.2017-0047.
[28]	尚小红, 周慧文, 严华兵, 等. 木薯块根肉质颜色基因CAPS标记的开发与验证[J/OL]. 分子植物育种, 2018, 16(3): 873-879[2021-11-01]. https://doi.org/CNKI:SUN:FZZW.0.2018-03-030.
	Shang Xiaohong, Zhou Huiwen, Yan Huabing, et al. Development and verification of a CAPS marker for color gene in cassava tuber[J/OL]. Molecular Plant Breeding, 2018, 16(3): 873-879[2021-11-01]. https://doi.org/CNKI:SUN:FZZW.0.2018-03-030.
[29]	张素君, 李兴河, 唐丽媛, 等. 陆地棉纤维品质性状关联分析及优异等位基因挖掘[J/OL]. 植物遗传资源学报, 2021, 22(1): 214-228[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.20200409003.
	Zhang Sujun, Li Xinghe, Tang Liyuan, et al. Exploration of elite alleles related with fibre quality traits in Gossypium hirsutum L. by association analysis[J/OL]. Journal of Plant Genetic Resources, 2021, 22(1): 214-228[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.20200409003.
[30]	张素君, 唐丽媛, 李兴河, 等. 陆地棉产量及农艺性状的SSR标记关联分析[J/OL]. 棉花学报, 2019, 31(5): 414-426[2021-11-01]. https://doi.org/10.11963/1002-7807.zsjzjh.20190911.
	Zhang Sujun, Tang Liyuan, Li Xinghe, et al. Association analysis of agronomic and yield-related traits in an upland cotton (Gossypium hirsutum L.) germplasm population based on SSR markers[J/OL]. Cotton Science, 2019, 31(5): 414-426[2021-11-01]. https://doi.org/10.11963/1002-7807.zsjzjh.20190911.
[31]	张素君, 唐丽媛, 李兴河, 等. SSR标记与陆地棉田间黄萎病抗性的关联分析[J/OL]. 华北农学报, 2018, 33(6): 152-159[2021-11-01]. https://doi.org/10.7668/hbnxb.2018.06.021.
	Zhang Sujun, Tang Liyuan, Li Xinghe, et al. Association analysis of Verticillium wilt-resistance in upland cotton germplasm population based on SSR markers[J/OL]. Acta Agriculturae Boreali-Sinica, 2018, 33(6): 152-159[2021-11-01]. https://doi.org/10.7668/hbnxb.2018.06.021.
[32]	朱荷琴, 吴征彬, 邹奎, 等. 国家棉花品种区域试验抗枯黄萎病鉴定方法[J/OL]. 中国棉花, 2007, 34(11): 9-24[2021-11-01]. https://doi.org/10.3969/j.issn.1000-632X.2007.11.003.
	Zhu Heqin, Wu Zhengbin, Zou Kui, et al. Technical proposal for identification of resistance to Verticillium wilt in national cotton variety regional test[J/OL]. China Cotton, 2007, 34(11): 9-24[2021-11-01]. https://doi.org/10.3969/j.issn.1000-632X.2007.11.003.
[33]	Chen Z J, Sreedasyam A, Ando A, et al. Genomic diversifications of five Gossypium allopolyploid species and their impact on cotton improvement[J/OL]. Nature Genetics, 2020, 52(5): 525-533[2021-11-01]. https://doi.org/10.1038/s41588-020-0614-5.
[34]	Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis[J/OL]. Plant Molecular Biology Reporter, 1993, 11(2): 122-127[2021-11-01]. https://doi.org/10.1007/BF02670470.
[35]	张军, 武耀廷, 郭旺珍, 等. 棉花微卫星标记的PAGE/银染快速检测[J/OL]. 棉花学报, 2000, 12(5): 267-269[2021-11-01]. https://doi.org/10.3969/j.issn.1002-7807.2000.05.012.
	Zhang Jun, Wu Yaoting, Guo Wangzhen, et al. Fast screening of microsatellite markers in cotton with PAGE/silver staining[J/OL]. Cotton Science, 2000, 12(5): 267-269[2021-11-01]. https://doi.org/10.3969/j.issn.1002-7807.2000.05.012.
[36]	李小胜, 陈珍珍. 如何正确应用SPSS软件做主成分分析[J/OL]. 统计研究, 2010, 27(8): 105-108[2021-11-01]. https://doi.org/10.3969/j.issn.1002-4565.2010.08.016.
	Li Xiaosheng, Chen Zhenzhen. How to apply SPSS software correctly to principal component analysis[J/OL]. Statistical Research, 2010, 27(8): 105-108[2021-11-01]. https://doi.org/10.3969/j.issn.1002-4565.2010.08.016.
[37]	Sariful I M, Thyssen G N, Jenkins J N, et al. A MAGIC population-based genome-wide association study reveals functional association of GhRBB1_A07 gene with superior fiber quality in cotton[J/OL]. BMC Genomics, 2016, 17(1): 903[2021-11-01]. https://doi.org/10.1186/s12864-016-3249-2.
[38]	钱能. 陆地棉遗传多样性与育种目标性状基因(QTL)的关联分析[D]. 南京: 南京农业大学, 2009.
	Qian Neng. Genetic diversity and association analysis of gene (QTL) of breeding target traits of upland cotton[D]. Nanjing: Nanjing Agricultural University, 2009.
[39]	陈光, 杜雄明. 我国陆地棉基础种质表型性状的遗传多样性分析[J/OL]. 西北植物学报, 2006(8): 1649-1656[2021-11-01]. https://doi.org/10.3321/j.issn:1000-4025.2006.08.023.
	Chen Guang, Du Xiongming. Genetic diversity of basal germplasm phenotypes in upland cotton in China[J/OL]. Acta Botanica Boreali-Occidentalia Sinica, 2006(8): 1649-1656[2021-11-01]. https://doi.org/10.3321/j.issn:1000-4025.2006.08.023.
[40]	代攀虹, 孙君灵, 何守朴, 等. 陆地棉核心种质表型性状遗传多样性分析及综合评价[J/OL]. 中国农业科学, 2016, 49(19): 3694-3708[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2016.19.003.
	Dai Panhong, Sun Junling, He Shoupu, et al. Comprehensive evaluation and genetic diversity analysis of phenotypic traits of core collection in upland cotton[J/OL]. Scientia Agricultura Sinica, 2016, 49(19): 3694-3708[2021-11-01]. https://doi.org/10.3864/j.issn.0578-1752.2016.19.003.
[41]	李慧琴, 于娅, 王鹏, 等. 270份陆地棉种质资源农艺性状与品质性状的遗传多样性分析[J/OL]. 植物遗传资源学报, 2019, 20(4): 903-910[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.20181025003.
	Li Huiqin, Yu Ya, Wang Peng, et al. Genetic diversity analysis of the main agronomic and fiber quality characteristics in 270 upland cotton germplasm resources[J/OL]. Journal of Plant Genetic Resources, 2019, 20(4): 903-910[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.20181025003.
[42]	尹会会, 李秋芝, 李海涛, 等. 134份国外陆地棉种质主要农艺性状与纤维品质性状的遗传多样性分析[J/OL]. 植物遗传资源学报, 2017, 18(6): 1105-1115[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.2017.06.012.
	Yin Huihui, Li Qiuzhi, Li Haitao, et al. Analysis of genetic diversity of the main agronomic and fibre quality characters of 134 foreign upland cotton germplasms[J/OL]. Journal of Plant Genetic Resources, 2017, 18(6): 1105-1115[2021-11-01]. https://doi.org/10.13430/j.cnki.jpgr.2017.06.012.
[43]	朱亚娟, 王鹏, 郭旺珍, 等. 利用海岛棉染色体片段导入系定位衣分和籽指QTL[J/OL]. 作物学报, 2010, 36(8): 1318-1323[2021-11-01]. https://doi.org/10.3724/SP.J.1006.2010.01318.
	Zhu Yajuan, Wang Peng, Guo Wangzhen, et al. Mapping QTL for lint percentage and seed index using Gossypium barbadense chromosome segment introgression lines[J/OL]. Acta Agronomica Sinica, 2010, 36(8): 1318-1323[2021-11-01]. https://doi.org/10.3724/SP.J.1006.2010.01318.
[44]	杜雄明, 刘方, 王坤波, 等. 棉花种质资源收集鉴定与创新利用[J/OL]. 棉花学报, 2017, 29(S1): 51-61[2021-11-01]. https://doi.org/10.11963/1002-7807.dxmdxm.20170825.
	Du Xiongming, Liu Fang, Wang Kunbo, et al. Collection evaluation and utilization of cotton germplasm[J/OL]. Cotton Science, 2017, 29(S1): 51-61[2021-11-01]. https://doi.org/10.11963/1002-7807.dxmdxm.20170825.
[45]	杜军凯, 余桂红, 王秀娥, 等. 赤霉病主效抗性QTL区域SSCP标记的发掘与验证[J/OL]. 麦类作物学报, 2010, 30(5): 829-834[2021-11-01]. https://doi.org/10.7606/j.issn.1009-1041.2010.05.009.
	Du Junkai, Yu Guihong, Wang Xiue, et al. Development and validation of a SSCP marker for fusarium head blight resistance QTL region in wheat[J/OL]. Journal of Triticeae Crops, 2010, 30(5): 829-834[2021-11-01]. https://doi.org/10.7606/j.issn.1009-1041.2010.05.009.
[46]	邓先萍. 陆地棉栽培品种与野生种系尖斑棉杂交群体产量及纤维品质QTL定位[D]. 重庆:西南大学, 2019.
	Deng Xianping. QTL maping for yield and fiber quality traits from (G. hirsutum cultivar × G. hirsutum landrace punctatum) population[D]. Chongqing: Southwest University, 2019.
[47]	Hasan N, Choudhary S, Naaz N, et al. Recent advancements in molecular marker-assisted selection and applications in plant breeding programs[J/OL]. Journal of Genetic Engineering and Biotechnology, 2021, 19(1): 128[2021-11-01]. https://doi.org/10.1007/s10681-007-9497-0.
[48]	Sangeeta S, Ramesh C, Anuj P, et al. Analysis of genetic diversity among papaya cultivars using Single Primer Amplification Reaction (SPAR) methods[J/OL]. The Journal of Horticultural Science and Biotechnology, 2005, 80(3): 291-296[2021-11-01]. https://doi.org/10.1080/14620316.2005.11511932.
[49]	Karihaloo J L, Dwivedi Y K, Archak S, et al. Analysis of genetic diversity of Indian mango cultivars using RAPD markers[J/OL]. The Journal of Horticultural Science and Biotechnology, 2003, 78(3): 285-289[2021-11-01]. https://doi.org/10.1080/14620316.2003.11511619.
[50]	刘志文, 傅廷栋, 刘雪平, 等. 作物分子标记辅助选择的研究进展、影响因素及其发展策略[J]. 植物学报, 2005, 22(S1): 82-90.
	Liu Zhiwen, Fu Yandong, Liu Xueping, et al. Advances, influence factors and development strategies in crop molecular-assisted selection[J]. Chinese Bulletin of Botany, 2005(S1): 82-90.
[51]	Ma Z Y, He S P, Wang X F, et al. Resequencing a core collection of upland cotton identifies genomic variation and loci influencing fiber quality and yield[J/OL]. Nature Genetics, 2018, 50(6): 803-813[2021-11-01]. https://doi.org/10.1038/s41588-018-0119-7.
[52]	李付广, 袁有禄. 棉花分子育种进展与展望[J/OL]. 中国农业科技导报, 2011, 13(5): 1-8[2021-11-01]. https://doi.org/10.3969/j.issn.1008-0864.2011.05.01.
	Li Fuguang, Yuan Youlu. Progress and prospect of cotton molecular breeding[J/OL]. Journal of Agricultural Science and Technology, 2011, 13(5): 1-8[2021-11-01]. https://doi.org/10.3969/j.issn.1008-0864.2011.05.01.

性状 Traits	SSR标记 SSR marker	染色体 Chromosome	最佳比对区间 Best matched position/bp
株高 Plant height	BNL2448	A13
	MUCS162	A3
	HAU1951	D2
单株铃数Boll number per plant	BNL1646	A12
衣分 Lint percentage	BNL193	D13
铃重 Boll weight	BNL2632	D1
	BNL2651	D13
	JESPR220	D8
	BNL2440	A5
	BNL226	D2/A3
	DPL0492	D10
黄萎病相对抗病指数 Relative disease index of Verticillium wilt	BNL3442	A11
纤维上半部平均长度 Fiber upper half mean length、断裂比强度 Fiber strength	CM45
纤维上半部平均长度 Fiber upper half mean length、断裂比强度 Fiber strength	NAU5387	D13
纤维上半部平均长度	CGR6528	A3
Fiber upper half mean length	JESPR152	D1	62 160 134 ~ 62 160 369
	NAU4057	D4	3 784 404 ~ 3 784 607
	NAU2697	D13	4 541 853 ~ 4 542 007
纤维上半部平均长度 Fiber upper half mean length、马克隆值 Micronaire value	MUSS162	A3
马克隆值 Micronaire value	BNL1694	D7
	DPL0886	D12	62 405 645 ~ 62 405 785
	NAU943	A12
马克隆值Micronaire value 断裂伸长率Breaking fiber elongation	NAU3607	A5
断裂伸长率	HAU1774	D6
Breaking fiber elongation	BNL1151	A11
	BNL2646	D1	41 193 820 ~ 41 193 961

性状 Traits	变异范围Range of variation			均值±标准差Mean±SD			变异系数CV/%
性状 Traits	2019	2020	BLUP	2019	2020	BLUP	2019	2020	BLUP
株高Plant height/cm	40.10 ~ 156.60	41.20 ~ 147.20	41.20 ~ 157.64	96.47±17.22	89.55±10.61	94.82±8.57	17.85	11.85	9.04
果枝数Fruit branch number per plant	6.22 ~ 19.40	6.10 ~ 23.40	6.10 ~ 23.40	11.53±1.51	12.35±1.45	12.34±1.45	13.10	11.74	11.75
铃数Boll number per plant	5.10 ~ 24.20	5.20 ~ 30.50	5.20 ~ 30.50	15.28±4.02	15.60±3.96	15.69±4.13	26.31	25.38	26.32
铃重Boll weight/g	3.34 ~ 7.62	3.46 ~ 7.97	3.44 ~ 7.74	5.57±0.60	5.82±0.70	5.69±0.58	10.77	12.03	7.84
衣分Lint percentage/%	28.50 ~ 48.15	20.16 ~ 48.57	20.16 ~ 48.57	40.11±3.33	40.16±3.68	40.19±3.15	8.30	9.16	10.19
纤维上半部平均长度 Fiber upper half mean length/mm	23.70 ~ 34.60	23.30 ~ 38.69	23.64 ~ 38.69	28.66±1.73	28.44±2.01	28.56±1.58	6.04	7.07	5.53
断裂比强度 Fiber strength/（cN·tex^-1）	22.10 ~ 42.80	18.00 ~ 44.80	18.00 ~ 39.27	30.58±3.55	30.36±3.71	30.47±2.76	11.61	12.22	9.06
马克隆值Micronaire value	3.53 ~ 6.30	2.66 ~ 6.30	2.66 ~ 6.08	5.28±0.49	5.22±0.52	5.24±0.43	9.28	9.96	8.21
断裂伸长率 Breaking elongation/%	5.13 ~ 8.23	5.49 ~ 8.37	5.49 ~ 8.37	6.72±0.40	6.80±0.39	6.80±0.38	5.95	5.74	5.59
纤维长度整齐度 Uniformity index/%	76.50 ~ 86.70	78.80 ~ 89.10	81.16 ~ 86.17	83.42±1.63	84.56±1.74	84.01±0.75	1.95	2.06	0.89
黄萎病相对病情指数 Relative disease index of Verticillium wilt/%	0.00 ~ 79.69	0.00 ~ 85.40	0.00 ~ 85.00	26.56±15.55	35.23±20.63	30.97±16.03	58.55	58.56	51.76

	PH	FBN	BN	BW	LP	FUHML	FS	MV	FE	FU
FBN	0.139^**
BN	0.234^**	0.203^**
BW	0.149^**	0.133^**	-0.060
LP	-0.028	0.160^**	0.113^*	0.114^*
FUHML	-0.025	0.060	-0.040	-0.007	-0.282^**
FS	-0.010	-0.030	-0.050	-0.038	-0.274^**	0.730^**
MV	0.045	0.155^**	0.060	0.295^**	0.534^**	-0.391^**	-0.292^**
FE	-0.220^**	0.090	0.030	-0.113^*	0.137^**	0.014	-0.041	-0.009
FU	-0.057	0.020	0.070	-0.010	-0.030	0.506^**	0.450^**	-0.130^**	0.167^**
RDI	-0.203^**	-0.233^**	-0.230^**	-0.201^**	-0.109^*	-0.040	0.006	-0.225^**	-0.026	-0.023

分组	株高Plant height/cm	单株果枝数 Fruit branch number per plant	单株铃数 Boll number per plant	铃重 Boll weight/g	衣分 Lint percentage/%	纤维上半部平均长度 Fiber upper half mean length/mm	断裂比强度 Fiber strength/（cN·tex^-1）	马克隆值Micro- naire value	断裂伸长率Breaking elongation/%	长度整齐度指数Uniformity index/%	黄萎病相对病情指数 Relative disease index of Verticillium wilt
I	95.18	12.39	15.75	5.69	40.24	28.53	30.42	5.25	6.80	83.99	30.71
II	94.74	12.33	15.79	5.69	40.18	28.55	30.47	5.24	6.81	84.02	30.91
III	112.86	9.50	17.50	5.74	38.55	28.01	31.54	5.41	6.74	83.76	21.25

典型材料 Typical material	优异表型及聚合优异位点 Elite phenotypic trait and allele	株高 Plant height/cm	铃重 Boll weight/g	纤维上半部平均长度 Fiber upper half mean length/mm	马克隆值 Micronaire value	断裂伸长率 Fiber breaking elongation/%
冀棉128 Jimian 128	FUHML: CM45-140+CGR6528-180+JESPR152-125	109.50	5.74	35.14	3.55	6.21
冀棉2016 Jimian 2016	FUHML: CM45-140+CGR6528-180+JESPR152-125	114.00	5.82	30.92	4.11	6.30
2011NWF	FUHML: CM45-140+CGR6528-180+JESPR152-125; MV: DPL0886-158	100.00	5.18	35.27	3.81	6.30
JMX-18	BW: JESPR220-160+BNL2440-240+BNL226-320	93.10	7.74	28.12	5.78	6.67
ZXJDL F6	BW: JESPR220-160+BNL2440-240+BNL226-320	99.28	7.47	28.24	5.45	6.67
12-6034	BW: JESPR220-160+BNL2440-240+BNL226-320	90.00	7.42	30.21	5.00	8.35
冀3927 Ji 3927	BW: JESPR220-160+BNL2440-240+BNL226-320	98.10	7.02	29.16	5.63	6.70
金陆长1号 Jinluchang 1	FUHML: CM45-140+JESPR152-125	109.31	5.31	32.67	4.22	6.78
新陆早66号 Xinluzao 66	FUHML: JESPR152-125+CM45-140+CGR6528-180	96.46	5.01	31.66	5.17	6.81
新陆中36号 Xinluzhong 36	FUHML: JESPR152-125+CM45-140+CGR6528-180	94.00	5.12	31.26	3.96	6.11
新陆中7号 Xinluzhong 7	FE: NAU3607-250+HAU1774-320+ BNL1151-210	95.40	5.97	29.46	4.55	8.03
光子资源 Guangziziyuan	FE: NAU3607-250+HAU1774-320	79.50	5.07	26.98	5.26	8.24
2011NWM	FE: NAU3607-250+HAU1774-320	91.80	4.22	27.82	5.33	8.37
鲁棉研27 Lumianyan 27	FE: NAU3607-250+HAU1774-320+BNL1151-210	85.70	5.92	28.96	5.18	7.94