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棉花学报 ›› 2022, Vol. 34 ›› Issue (2): 120-136.doi: 10.11963/cs20210066

• 研究与进展 • 上一篇    下一篇

陆地棉主要育种性状SSR关联位点的验证及优异材料鉴定(2022年11月8日更新)

张素君(),李兴河(),王海涛,唐丽媛,蔡肖,刘存敬,张香云*(),张建宏*()   

  1. 河北省农林科学院棉花研究所/农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室/国家棉花改良中心河北分中心,石家庄 050051
  • 收稿日期:2021-11-16 出版日期:2022-03-15 发布日期:2022-07-19
  • 通讯作者: 张香云 jimianzhang@qq.com;张建宏 mhszjh@126.com
  • 作者简介:张素君(1987―),硕士,sujun1987_good@126.com|李兴河(1989―)硕士,1240296052@qq.com
  • 基金资助:
    河北省农林科学院科技创新专项(2022KJCXZX-MHS-1);河北省自然科学基金青年基金(C2021301039);棉花生物学国家重点实验室开放课题(CB2021A31);河北省农林科学院基本科研业务费基金项目(2021070204)

Verification of SSR-associated loci for main breeding traits and identification of excellent germplasms in upland cotton (Gossypium hirsutum L.)

Zhang Sujun(), Li Xinghe(), Wang Haitao, Tang Liyuan, Cai Xiao, Liu Cunjing, Zhang Xiangyun*(), Zhang Jianhong*()   

  1. Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Cotton Biology and Genetics and Breeding in Huanghuai-Hai Semi-arid Region, National Cotton Improvement Center Hebei Branch, Shijiazhuang 050051, China
  • Received:2021-11-16 Online:2022-03-15 Published:2022-07-19
  • Contact: Zhang Xiangyun jimianzhang@qq.com;Zhang Jianhong mhszjh@126.com

摘要:

【目的】 评价与陆地棉纤维品质、产量、黄萎病抗性等主要育种性状关联的简单重复序列(simple sequence repeat, SSR)位点的选择效果,并挖掘优异典型材料。【方法】 在474份陆地棉中,利用双尾t-检验在26个关联标记中筛选具有显著筛选效果的变异位点,并鉴定典型材料。【结果】 共检测到30个多态性位点,基于上述位点可将其中420份陆地棉材料完全区分。20个变异位点与目标性状显著(P<0.05)关联,其中,与铃重和纤维长度密切关联的变异位点各6个,与马克隆值、伸长率、株高和铃数关联的位点分别有3、3、1和1个。获得了与铃重(JESPR220-160+BNL2440-240+BNL226-320)和纤维长度(CM45-140+CGR6528-180+JESPR152-125)密切关联的分子标记组合各1个,表型效应分别达0.27 g和1.27 mm,根据上述标记位点鉴定优异典型材料14份。【结论】 获得了具有显著筛选效果的多态性位点及优异典型材料,可为棉花分子标记辅助选择及组合选配提供参考。

关键词: 陆地棉; 表型性状; SSR位点; 筛选效果; 典型材料

Abstract:

[Objective] This study aims to evaluate and validate SSR (simple sequence repeat) markers associated with traits of fiber quality, yield, and resistance to Verticillium wilt of upland cotton, and identify typical materials carrying elite alleles. [Method] Among the 26 SSR markers identified from previous studies, the two-tailed t-test was used to screen the mutation sites with significant effect on phenotypic variations of 474 upland cotton materials, and to identify typical materials. [Result] A total of 30 polymorphic loci were detected, and can be used to fully differentiate 420 upland cotton materials. Interestingly, 20 mutation sites were significantly associated with target traits (P<0.05), of which, 6 mutation sites were closely related to boll weight and fiber length, respectively. Moreover, there are 3, 3, 1, and 1 loci associated with micronaire value, breaking elongation, plant height and boll number, respectively. And, the marker combinations (JESPR220-160+BNL2440-240+BNL226-320) and (CM45-140+CGR6528-180+JESPR152-125) were associated with the boll weight and fiber length, with phenotypic effect of 0.27 g and 1.27 mm, respectively. Furthermore, 14 typical materials carrying elite alleles were obtained. [Conclusions] In this study, polymorphic sites with significant phenotypic effects and typical materials of elite alleles were obtained, which provided references for cotton molecular marker-assisted selection and material selection.

Key words: upland cotton; phenotypic traits; SSR locus; screening effect; typical material