欢迎访问《棉花学报》! 今天是

棉花学报 ›› 2022, Vol. 34 ›› Issue (2): 107-119.doi: 10.11963/cs20210060

• 研究与进展 • 上一篇    下一篇

棉花SRS基因家族的全基因组鉴定及生物信息学分析

张雪(),孙瑞斌(),马聪聪,马丹,张晓睿,刘志红,刘传亮*()   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳,455000
  • 收稿日期:2021-10-13 出版日期:2022-03-15 发布日期:2022-05-30
  • 通讯作者: 刘传亮 E-mail:liucl1971@126.com
  • 作者简介:张雪(1994―),女,硕士研究生, xue_zhang0912@163.com|孙瑞斌(1991―),男,硕士研究生, 381183463@qq.com
  • 基金资助:
    棉花生物学国家重点实验室自主课题(CB2021C15)

Genome-wide identification and bioinformatic analysis of SRS gene family in cotton

Zhang Xue(), Sun Ruibin(), Ma Congcong, Ma Dan, Zhang Xiaorui, Liu Zhihong, Liu Chuanliang*()   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2021-10-13 Online:2022-03-15 Published:2022-05-30
  • Contact: Liu Chuanliang E-mail:liucl1971@126.com

摘要:

【目的】 短链相关序列(SHI-related sequence, SRS)家族是一类植物特有的转录因子,在调节植物生长发育和逆境胁迫反应中发挥重要作用。鉴定和分析棉花SRS基因家族成员。【方法】 结合拟南芥相关信息,以陆地棉和海岛棉为主要研究对象,对棉花SRS基因进行全基因组鉴定和系统进化研究,并分析其在不同器官、不同发育时期的胚珠和纤维、以及在非生物胁迫下棉苗中的表达模式。【结果】 在陆地棉和海岛棉中分别鉴定出27个和26个SRS基因,系统进化分析将棉花SRS基因家族成员划分为3个分支,不同分支都具有相似的保守基序。每个分支都存在多对同源基因。一些SRS基因在胚珠发育时期表达量较高,并响应多种非生物胁迫。【结论】 分析预测了棉花SRS基因的家族成员进化关系与潜在功能,为棉花SRS基因的功能研究提供初步的理论基础。

关键词: 棉花; SRS; 系统进化; 表达模式; 基因家族

Abstract:

[Objective] SHI-related sequence (SRS) family is a group of plant-specific transcription factors that play important roles in regulating plant development and response to adversity stress. However, the function of SRS gene family in cotton has not been well characterized. [Method] Based on the published genomic data of Gossypium hirsutum, G. barbadense and Arabidopsis thaliana, the genome-wide identification and phylogenetic analysis of SRS genes in cotton were conducted. And the expression pattern of SRS genes in ovule and fiber at different developmental stages, in different organs and under different abiotic stress conditions were analyzed. [Result] In total, 27 and 26 SRS genes were identified in G. hirsutum and G. barbadense, respectively. According to the phylogenetic analysis, SRS genes in cotton could be divided into three groups, and different groups had similar conserved motifs. There were multiple pairs of homologous genes in each subgroup. Some SRS genes were highly expressed in ovules at different developmental stages, and were also responsive to various abiotic stresses. [Conclusion]The evolution and potential functions of SRS gene family were analyzed and predicted, which provided a theoretical basis for the functional analysis of SRS genes in cotton.

Key words: cotton; SRS; phylogeny; expression pattern; gene family