欢迎访问《棉花学报》! 今天是

棉花学报 ›› 2022, Vol. 34 ›› Issue (1): 69-78.doi: 10.11963/cs20210055

• 研究简报 • 上一篇    

新疆石河子及周边地区棉花根际土壤丛枝菌根真菌多样性

陈凯丽(),田秋恒,刘志洋,王海,熊杰,雷勇辉,孙燕飞*()   

  1. 石河子大学,新疆 石河子 832003
  • 收稿日期:2021-09-03 出版日期:2022-01-15 发布日期:2022-05-31
  • 通讯作者: 孙燕飞 E-mail:1343873397@qq.com;81711308@qq.com
  • 作者简介:陈凯丽(1996―),女,研究生, 1343873397@qq.com
  • 基金资助:
    国家重大基础研究专项(2016YFC0501404)

Diversity of arbuscular mycorrhizal fungi in cotton rhizosphere soil in Shihezi and surrounding areas, Xinjiang

Chen Kaili(),Tian Qiuheng,Liu Zhiyang,Wang Hai,Xiong Jie,Lei Yonghui,Sun Yanfei*()   

  1. Shihezi University, Shihezi, Xinjiang 832003, China
  • Received:2021-09-03 Online:2022-01-15 Published:2022-05-31
  • Contact: Sun Yanfei E-mail:1343873397@qq.com;81711308@qq.com

摘要:

【目的】丛枝菌根是陆地生态系统中普遍存在的植物根系与丛枝菌根真菌的共生体,能有效提高植物的抗逆性。棉花是新疆盐碱地种植的主要经济作物。为研究和利用新疆丛枝菌根资源,对新疆石河子及其周边棉花种植区丛枝菌根真菌的种类和数量进行了普查。【方法】采集新疆维吾尔自治区石河子市(北泉镇)及其周边的塔城地区沙湾县(柳毛湾镇、老沙湾镇、钟家庄镇)和昌吉回族自治州玛纳斯县(十户滩镇)共50份土样,通过单个孢子的形态学鉴定和分子鉴定(巢式聚合酶链式反应)对丛枝菌根真菌多样性进行分析。【结果】从新疆石河子及周边地区棉花根际土壤中分离出4属6种丛枝菌根真菌,以类球囊霉属(Paraglomus)为优势属。石河子市北泉镇的丛枝菌根真菌物种丰富度最高,沙湾县柳毛湾镇和钟家庄镇的种类组成和结构相似。【结论】明确了新疆石河子以及周边地区棉花根际土壤中丛枝菌根真菌的种类。该研究为充分利用和开发特色丛枝菌根资源奠定了基础。

关键词: 棉花; 丛枝菌根真菌; 多样性; 单孢鉴定; 巢式聚合酶链式反应

Abstract:

[Objective] Arbuscular mycorrhiza (AM) are symbols of plant roots and arbuscular mycorrhizal fungi (AMF) that are ubiquitous in terrestrial ecosystems, which can effectively improve the drought resistance and salt tolerance of plants. Cotton is a major cash crop grown in saline lands in Xinjiang. In order to study and utilize the AMF resources of Xinjiang, a census was conducted on the species and population of AMF in cotton growing areas in Shihezi and surrounding areas of Xinjiang. [Method] Fifty soil samples were collected from three cities/counties in Xinjiang Uygur Autonomous Region, and the diversity of AMF was analyzed by morphological and molecular identification (Nested polymerase chain reaction). [Result] There are six species of four genera AMF isolated from the rhizasphere soil of Shihezi and surrounding areas, with Paraglomus as the dominant genera. The highest species diversity of AMF was found in Beiquan Town of Shihezi City, and the species composition and structure were similar in Liumaowan Town, Zhongjiazhuang Town, and Shawan County. [Conclusion] The species of AMF isolated from the rhizasphere soil of Shihezi and surrounding areas was determined. The study provides a theoretical basis for the full utilization and exploitation of the characteristic AMF resources of cotton in Xinjiang.

Key words: cotton; arbuscular mycorrhizal fungi; diversity; single spore identification; nested polymerase chain reaction