陆地棉UDPGP基因家族的鉴定及抗旱性分析

doi:10.11963/cs20200100

摘要/Abstract

摘要：

【目的】尿苷二磷酸葡萄糖焦磷酸化酶（Uridine diphosphate glucose pyrophosphorylase, UDPGP）是植物碳水化合物代谢和细胞壁生物合成中的关键酶,但关于棉花UDPGP基因研究较少。对陆地棉UDPGP基因进行全基因组分析,为深入研究UDPGP基因在棉花抗逆中的作用提供参考。【方法】利用已公布的陆地棉标准系TM-1基因组数据,通过生物信息学分析方法对陆地棉UDPGP基因进行全基因组鉴定,分析该家族成员的理化性质、系统进化关系、基因结构、染色体定位、启动子区顺式作用元件、组织表达特异性和干旱胁迫下的表达模式。【结果】陆地棉UDPGP基因家族包含31个成员,不均等地分布在18条染色体上,可分为5个亚族。顺式作用元件分析发现,大部分UDPGP基因的启动子区域含有响应脱落酸、赤霉素、水杨酸、茉莉酸甲酯的顺式作用元件以及响应干旱的MYB结合位点。转录组分析表明,GhUDPGP10和GhUDPGP26基因响应干旱胁迫,且在棉花多个器官中较高水平表达;实时荧光定量聚合酶链式反应结果显示,干旱胁迫下,GhUDPGP10和GhUDPGP26在抗旱材料的根中表达量普遍较高;而在干旱敏感材料中,主要在叶中高表达,推测这两个基因在不同的遗传材料中抗逆作用机制不同。【结论】明确了陆地棉UDPGP基因家族成员的分布特征、结构特征以及系统进化特征,通过表达分析初步揭示了该家族GhUDPGP10和GhUDPGP26基因可能在棉花生长发育和抗旱响应中发挥功能,为解析GhUDPGP基因的抗旱分子机理奠定基础。

关键词: 陆地棉; 尿苷二磷酸葡萄糖焦磷酸化酶; 抗旱性; 表达模式

Abstract:

[Objective] Uridine diphosphate glucose pyrophosphorylase (UDPGP) is a key enzyme in plant carbohydrate metabolism and cell wall biosynthesis, but there are few studies on UDPGP genes in cotton. The purpose of this study is to conduct the genome-wide analysis of UDPGP in upland cotton and to provide a reference for further study on the role of UDPGP gene in stress resistance. [Method] Using the published TM-1 genome data, the UDPGP family genes of upland cotton were identified by bioinformatics analysis. Their physicochemical properties, evolutionary relation, gene structure, chromosome location, cis-acting elements in promoter region, tissue-specific expression and expression pattern under drought stress were analyzed. [Result] There are 31 UDPGP genes in upland cotton, which are unevenly distributed on 18 chromosomes and can be divided into 5 subgroups. Most of the promoter regions of UDPGP genes contained cis-acting elements in response to abscisic acid, gibberellin, salicylic acid, methyl jasmonate and MYB-binding site (MBS) involved in drought response. Transcriptome analysis showed that GhUDPGP10 and GhUDPGP26 genes responded to drought stress and were highly expressed in many tissues of cotton. The results of quantitative real-time polymerase chain reaction showed that GhUDPGP10 and GhUDPGP26 were highly expressed in the roots at 3-24 h after stress of drought resistant materials and mainly high expressed in the leaves of drought sensitive materials under drought stress. It is speculated that the stress resistance mechanisms of these two genes differ from each other in different genetic materials. [Conclusion] This study clarified the distribution, structural and phylogenetic characteristics of UDPGP genes in upland cotton. According to the expression analysis, GhUDPGP10 and GhUDPGP26 genes may involve in cotton growth and drought resistance, which lays a foundation for the subsequent analysis of the molecular mechanisms of GhUDPGP genes in drought resistance.

Key words: Gossypium hirsutum L.; uridine diphosphate-glucose pyrophosphorylase; drought resistance; expression profile

陈琴,李多露,赵杰银,高文举,陈全家,曲延英. 陆地棉UDPGP基因家族的鉴定及抗旱性分析[J]. 棉花学报, 2022, 34(1): 12-22.

Chen Qin,Li Duolu,Zhao Jieyin,Gao Wenju,Chen Quanjia,Qu Yanying. Identification and drought resistance analysis of UDPGP family genes in Gossypium hirsutum[J]. Cotton Science, 2022, 34(1): 12-22.

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链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/cs20200100

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2022/V34/I1/12

图/表 6

表1

表2

陆地棉UDPGP基因信息"

基因编号 Gene ID	基因名称 Gene name	染色体位置 Chromosome location	ORF长度 ORF length/bp	蛋白长度 Protein length/aa	蛋白分子质量 Protein size/ku	等电点 Isoelectric point	亚细胞定位 Subcellular localization
GH_A02G1221	GhUDPGP01	A02: 65 445 728 ~ 65452745	1 884	627	69.28	6.12	Ext
GH_A03G2444	GhUDPGP02	A03: 111 508 218 ~ 111 514 524	1 473	490	54.63	6.25	Cyt
GH_A05G2382	GhUDPGP03	A05: 23 629 292 ~ 23 641 014	1 965	654	72.78	6.64	Ext
GH_A07G1818	GhUDPGP04	A07: 55 230 148 ~ 55 230 916	273	90	10.01	9.91	Cyt
GH_A07G1819	GhUDPGP05	A07: 55 232 573 ~ 55 234 744	687	228	25.70	4.99	Cyt
GH_A08G0422	GhUDPGP06	A08: 4 575 002 ~ 4 581 575	1 404	467	51.50	5.86	Cyt
GH_A08G2499	GhUDPGP07	A08: 121 814 292 ~ 121 819 836	1 884	627	68.83	6.69	Ext
GH_A10G1000	GhUDPGP08	A10: 19 797 228 ~ 19 802 476	1 866	621	68.62	6.93	Ext
GH_A10G2644	GhUDPGP09	A10: 114 588 353 ~ 114 608 136	2 628	875	96.66	6.05	Ext
GH_A11G1773	GhUDPGP10	A11: 22 289 514 ~ 22 294 382	1 398	465	51.31	5.62	Cyt
GH_A11G2500	GhUDPGP11	A11: 88 056 120 ~ 88 065 918	1 845	614	68.35	6.26	Cyt
GH_A12G0472	GhUDPGP12	A12: 9 341 477 ~ 9 347 072	1 506	501	55.04	6.12	Cyt
GH_D01G1492	GhUDPGP13	D01: 31 984 768 ~ 31 988 536	552	183	19.74	4.91	Ext
GH_D02G2606	GhUDPGP14	D02: 69 669 195 ~ 69 684 286	981	326	36.31	8.92	Cyt
GH_D03G0708	GhUDPGP15	D03: 19 170 452 ~ 19 177 329	1 884	627	69.27	6.78	Ext
GH_D03G1240	GhUDPGP16	D03: 42 297 024 ~ 42 297 435	258	85	9.69	9.51	Cyt
GH_D04G0520	GhUDPGP17	D04: 8 139 192 ~ 8 141 506	306	101	11.70	6.57	Ext
GH_D04G0553	GhUDPGP18	D04: 9 016 214 ~ 9 016 589	306	101	11.49	8.87	Cyt
GH_D07G1857	GhUDPGP19	D07: 37 319 315 ~ 37 320 305	348	115	12.73	7.85	Cyt
GH_D08G0444	GhUDPGP20	D08: 4 257 074 ~ 4 263 644	1 404	467	51.54	5.82	Cyt
GH_D08G0875	GhUDPGP21	D08: 15 108 176 ~ 15 110 861	324	107	11.27	8.47	Cyt
GH_D08G2508	GhUDPGP22	D08: 65 886 935 ~ 65 892 208	1 842	613	67.63	6.49	Ext
GH_D10G1795	GhUDPGP23	D10: 47 209 347 ~ 47 209 758	258	85	9.83	6.82	Cyt
GH_D10G2450	GhUDPGP24	D10: 62 181 672 ~ 62 182 823	492	163	18.05	9.71	Ext
GH_D10G2749	GhUDPGP25	D10: 66 423 070 ~ 66 427 939	2 610	869	9.66	6.00	Ext
GH_D11G1805	GhUDPGP26	D11: 18 727 608 ~ 18 732 182	1 398	465	51.30	5.45	Cyt
GH_D11G1888	GhUDPGP27	D11: 20 099 709 ~ 20 100 084	306	101	11.47	8.87	Cyt
GH_D11G2547	GhUDPGP28	D11: 48 854 774 ~ 48 860 882	1 845	614	68.42	6.36	Cyt
GH_D11G2621	GhUDPGP29	D11: 51 800 632 ~ 51 800 887	186	61	6.88	9.03	Cyt
GH_D12G0484	GhUDPGP30	D12: 7 230 507 ~ 7 236 086	1 512	503	55.18	6.12	Cyt
GH_D13G1555	GhUDPGP31	D13: 49 696 588 ~ 49 696 999	258	85	9.80	9.03	Cyt

表2

图1

图2

图3

图4

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引物名称 Primer name	引物序列 Primer sequence
GhUDPGP10-qt-F	CCTGAATTCAAGAAGGTTGGT
GhUDPGP10-qt-R	TTCGGGGACATTAATTTCCTT
GhUDPGP26-qt-F	CCTGAATTCAAGAAGGTTGGT
GhUDPGP26-qt-R	GGGGCCATTAATTTCCTT
GhUBQ7-qt-F	GACCTACACCAAGCCCAAGAAG
GhUBQ7-qt-R	TGAGCCCACACTTACCACAATAGT