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棉花学报 ›› 2022, Vol. 34 ›› Issue (1): 12-22.doi: 10.11963/cs20200100

• 研究与进展 • 上一篇    下一篇

陆地棉UDPGP基因家族的鉴定及抗旱性分析

陈琴(),李多露(),赵杰银,高文举,陈全家,曲延英*()   

  1. 新疆农业大学农学院/教育部棉花工程研究中心/新疆作物遗传改良与种质创新重点实验室,乌鲁木齐 830052
  • 收稿日期:2020-12-25 出版日期:2022-01-15 发布日期:2022-05-31
  • 通讯作者: 曲延英 E-mail:cqq0777@163.com;ldlfyx@126.com;xjyyq5322@126.com
  • 作者简介:陈琴(1982―),女,博士,副教授, cqq0777@163.com;|李多露(1995―),男,硕士, ldlfyx@126.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2021D01A92);中国博士后科学基金面上项目(地区专项支持计划)第69批(2021M693901);新疆维吾尔自治区重大科技专项(2020A01001-2)

Identification and drought resistance analysis of UDPGP family genes in Gossypium hirsutum

Chen Qin(),Li Duolu(),Zhao Jieyin,Gao Wenju,Chen Quanjia,Qu Yanying*()   

  1. College of Agronomy, Xinjiang Agricultural University/Cotton Engineering Research Center, Ministry of Education/Xinjiang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation, Urumqi 830052, China
  • Received:2020-12-25 Online:2022-01-15 Published:2022-05-31
  • Contact: Qu Yanying E-mail:cqq0777@163.com;ldlfyx@126.com;xjyyq5322@126.com

摘要:

【目的】尿苷二磷酸葡萄糖焦磷酸化酶(Uridine diphosphate glucose pyrophosphorylase, UDPGP)是植物碳水化合物代谢和细胞壁生物合成中的关键酶,但关于棉花UDPGP基因研究较少。对陆地棉UDPGP基因进行全基因组分析,为深入研究UDPGP基因在棉花抗逆中的作用提供参考。【方法】利用已公布的陆地棉标准系TM-1基因组数据,通过生物信息学分析方法对陆地棉UDPGP基因进行全基因组鉴定,分析该家族成员的理化性质、系统进化关系、基因结构、染色体定位、启动子区顺式作用元件、组织表达特异性和干旱胁迫下的表达模式。【结果】陆地棉UDPGP基因家族包含31个成员,不均等地分布在18条染色体上,可分为5个亚族。顺式作用元件分析发现,大部分UDPGP基因的启动子区域含有响应脱落酸、赤霉素、水杨酸、茉莉酸甲酯的顺式作用元件以及响应干旱的MYB结合位点。转录组分析表明,GhUDPGP10GhUDPGP26基因响应干旱胁迫,且在棉花多个器官中较高水平表达;实时荧光定量聚合酶链式反应结果显示,干旱胁迫下,GhUDPGP10GhUDPGP26在抗旱材料的根中表达量普遍较高;而在干旱敏感材料中,主要在叶中高表达,推测这两个基因在不同的遗传材料中抗逆作用机制不同。【结论】明确了陆地棉UDPGP基因家族成员的分布特征、结构特征以及系统进化特征,通过表达分析初步揭示了该家族GhUDPGP10GhUDPGP26基因可能在棉花生长发育和抗旱响应中发挥功能,为解析GhUDPGP基因的抗旱分子机理奠定基础。

关键词: 陆地棉; 尿苷二磷酸葡萄糖焦磷酸化酶; 抗旱性; 表达模式

Abstract:

[Objective] Uridine diphosphate glucose pyrophosphorylase (UDPGP) is a key enzyme in plant carbohydrate metabolism and cell wall biosynthesis, but there are few studies on UDPGP genes in cotton. The purpose of this study is to conduct the genome-wide analysis of UDPGP in upland cotton and to provide a reference for further study on the role of UDPGP gene in stress resistance. [Method] Using the published TM-1 genome data, the UDPGP family genes of upland cotton were identified by bioinformatics analysis. Their physicochemical properties, evolutionary relation, gene structure, chromosome location, cis-acting elements in promoter region, tissue-specific expression and expression pattern under drought stress were analyzed. [Result] There are 31 UDPGP genes in upland cotton, which are unevenly distributed on 18 chromosomes and can be divided into 5 subgroups. Most of the promoter regions of UDPGP genes contained cis-acting elements in response to abscisic acid, gibberellin, salicylic acid, methyl jasmonate and MYB-binding site (MBS) involved in drought response. Transcriptome analysis showed that GhUDPGP10 and GhUDPGP26 genes responded to drought stress and were highly expressed in many tissues of cotton. The results of quantitative real-time polymerase chain reaction showed that GhUDPGP10 and GhUDPGP26 were highly expressed in the roots at 3-24 h after stress of drought resistant materials and mainly high expressed in the leaves of drought sensitive materials under drought stress. It is speculated that the stress resistance mechanisms of these two genes differ from each other in different genetic materials. [Conclusion] This study clarified the distribution, structural and phylogenetic characteristics of UDPGP genes in upland cotton. According to the expression analysis, GhUDPGP10 and GhUDPGP26 genes may involve in cotton growth and drought resistance, which lays a foundation for the subsequent analysis of the molecular mechanisms of GhUDPGP genes in drought resistance.

Key words: Gossypium hirsutum L.; uridine diphosphate-glucose pyrophosphorylase; drought resistance; expression profile