棉花学报 ›› 2021, Vol. 33 ›› Issue (4): 360-367.doi: 10.11963/cs20200025
收稿日期:
2020-04-01
出版日期:
2021-07-15
发布日期:
2021-09-14
通讯作者:
张朝军
E-mail:1602094950@qq.com;chauncy_m@163.com;zcj1999@yeah.net
作者简介:
苟浩琦(1992―),女,硕士研究生, 基金资助:
Gou Haoqi(),Ma Changkai(
),Zhang Qian,Fan Shuli,Ma Qifeng,Zhang Chaojun(
)
Received:
2020-04-01
Online:
2021-07-15
Published:
2021-09-14
Contact:
Zhang Chaojun
E-mail:1602094950@qq.com;chauncy_m@163.com;zcj1999@yeah.net
摘要:
【目的】 培育棉花新型光敏不育系,获得优良光敏不育系亲本,为棉花育种提供优异亲本材料。【方法】 利用psm4与W10进行杂交,然后自交3代,获得与psm4具有相同光敏不育特性的光敏不育系psm5。研究psm5在不同光照条件下的育性及其转变规律。【结果】 psm5保留了psm4在日照时间大于12.5 h的条件下花药不开裂、不能正常散粉,而在日照时间小于11.5 h的短日照条件下花药可以正常开裂散粉、正常可育的特性。研究psm5在不同光照条件下的育性及其转变规律。【结论】 介绍了光敏雄性核不育新材料psm5的培育过程及其光敏特性,为利用psm5杂交制种提供了可靠的理论基础。psm5和psm4育性转变时期发生在开花前12~15 d。psm5与psm4一样是典型的孢子体光敏雄性不育系。psm5是隐性光敏核不育系,恢复系范围广,利于配制优势杂交组合,在棉花杂交育种中有良好的应用前景。
苟浩琦,马常凯,张迁,范术丽,马启峰,张朝军. 棉花光敏雄性不育系psm5的培育及其育性转变规律[J]. 棉花学报, 2021, 33(4): 360-367.
Gou Haoqi,Ma Changkai,Zhang Qian,Fan Shuli,Ma Qifeng,Zhang Chaojun. Breeding of the photosensitive male sterile line psm5 in cotton and the pattern of fertility transformation[J]. Cotton Science, 2021, 33(4): 360-367.
表1
不同温度和光周期条件下psm4与psm5育性转变规律"
不育系 Male sterile line | 性状 Trait | 35 ℃/20 ℃ | 25 ℃/15 ℃ | |||||
P1 | P1→P2 | P2→P1 | P1 | P1→P2 | P2→P1 | |||
psm4 | 花药是否开裂 Cracking situation of anthers | N | Y | N | N | Y | N | |
育性转变所需时间 The time of fertility transition/d | 12.2±1.92 | 12.4±2.30 | 18.4±3.05 | 18.4±1.81 | ||||
psm5 | 花药是否开裂 Cracking situation of anthers | N | Y | N | N | Y | N | |
育性转变所需时间 The time of fertility transition/d | 13.0±1.58 | 12.8±1.64 | 19.8±1.92 | 19.6±2.88 |
[1] | 李泽福, 夏加发. 植物雄性不育类型及其遗传机制的研究进展[J]. 安徽农业科学, 2000, 28(6):742-746. |
Li Zefu, Xia Jiafa. Advances in studies on the type and genetic mechanism of plant male sterility[J]. Journal of Anhui Agricultural Sciences, 2000, 28(6):742-746. | |
[2] | Galau G A, Wilkins T A. Alloplasmic male sterility in AD allotetraploid Gossypium hirsutum upon replacement of its resident A cytoplasm with that of D species G. harknessii[J]. Theoretical & Applied Genetics, 1989, 78(1):23-30. |
[3] | 马勇, 吴建勇, 邢朝柱, 等. 哈克尼西棉细胞质雄性不育系和保持系差异表达基因分析[J]. 中国农业科学, 2009, 42(10):3706-3712. |
Ma Yong, Wu Jianyong, Xing Chaozhu, et al. Differentially expressed genes between cytoplasmic male sterility lines and maintainer lines of Gossypium harknessii[J]. Scientia Agricultura Sinica. 2009, 42(10):3706-3712. | |
[4] |
Meyer V G, Meyer J R. Cytoplasmically controlled male sterility in cotton[J]. Crop Science, 1965, 5(5):444-448.
doi: 10.2135/cropsci1965.0011183X000500050021x |
[5] |
Meyer V G. Some effects of genes, cytoplasm, and environment on male sterility of cotton (Gossypium)[J]. Crop Science, 1969, 9(2):237-242.
doi: 10.2135/cropsci1969.0011183X000900020039x |
[6] | Stewart J M. A new cytoplasmic male sterile and restorer for cotton[C]// National Cotton Council. Proceedings of Beltwide Cotton Conference. Memphis: National Cotton Council, 1992: 610. |
[7] | 邢朝柱, 郭立平, 吴建勇, 等. 转基因抗虫三系杂交种——中棉所99[J]. 中国棉花, 2017, 44(2):25-26. |
Xing Chaozhu, Guo Liping, Wu Jianyong, et al. A transgenic insect-resistant cotton hybrid bred by cytoplasmic male-sterility system, CCRI 99[J]. China Cotton, 2017, 44(2):25-26. | |
[8] | 豆丽萍, 王庆亚, 唐灿明. 陆地棉双隐性核雄性不育系ms5ms6花药发育过程的研究[J]. 棉花学报, 2009, 21(4):265-270. |
Dou Liping, Wang Qingya, Tang Canming. The anther development research on double recessiveness nuclear male-sterile line ms5ms6 of Gossypium hirsutum L.[J]. Cotton Science, 2009, 21(4):265-270. | |
[9] | 张天真, 冯义军, 潘家驹. 我国发现的4个棉花核雄性不育系的遗传分析[J]. 棉花学报, 1992, 4(1):1-8. |
Zhang Tianzhen, Feng Yijun, Pan Jiaju. Genetic analysis of four male sterile lines of cotton kernel discovered in China[J]. Cotton Science, 1992, 4(1):1-8. | |
[10] |
Chen D, Ding Y, Guo W, et al. Molecular mapping of genic male-sterile genes ms15, ms5 and ms6 in tetraploid cotton[J]. Plant Breeding, 2009, 128(2):193-198.
doi: 10.1111/pbr.2009.128.issue-2 |
[11] |
Fankhauser C, Chory J. Light control of plant development[J]. Annual Review of Cell and Developmental Biology, 1997, 13(1):203-229.
doi: 10.1146/annurev.cellbio.13.1.203 |
[12] | 石明松. 晚粳自然两用系选育及应用初报[J]. 湖北农业科学, 1981, 7(1):1-3. |
Shi Mingsong. Preliminary report on breeding and application of late japonica natural dual-use system[J]. Hubei Agriculture Sciences, 1981, 7(1):1-3. | |
[13] | Zhou H, Zhou M, Yang Y Z, et al. RNase ZS1 processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice[J]. Nature Communications, 2014, 5(1):1-9. |
[14] | Xu J, Wang B, Wu Y, et al. Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)[J]. Theoretical & Applied Genetics, 2011, 122(2):365-372. |
[15] |
Zhou H, Liu Q, Li J, et al. Photoperiod-and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA[J]. Cell Research, 2012, 22(4):649-660.
doi: 10.1038/cr.2012.28 pmid: 22349461 |
[16] |
Zhang Hui, Xu Chenxi, He Yi, et al. Mutation in CSA creates a new photoperiod-sensitive genic male sterile line applicable for hybrid rice seed production[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(1):76-81.
doi: 10.1073/pnas.1213041110 pmid: 23256151 |
[17] | Jia J H, Zhang D S, Li C Y, et al. Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice[J]. Theoretical & Applied Genetics, 2001, 103(4):607-612. |
[18] | Tang J H, Fu Z Y, Li J S, et al. Genetic analyses and mapping of a new thermo-sensitive genic male sterile gene in maize[J]. Theoretical & Applied Genetics, 2006, 113(1):11-15. |
[19] | 马翎健, 宋喜悦, 胡银岗, 等. 光敏小麦雄性不育系A31的育性变异及遗传研究[J]. 西北农林科技大学学报(自然科学版), 2004, 32(4):5-8. |
Ma Lingjian, Song Xiyue, Hu Yingang, et al. Research on fertility variation and genetic characters of photoperiod-sensitive wheat male sterile line(A31)[J]. Journal of Northwest A&F University (Natural Science Edition), 2004, 32(4):5-8. | |
[20] | 何蓓如, 董普辉, 宋喜悦, 等. 小麦温度敏感不育系A3314温敏特性研究[J]. 麦类作物学报, 2003, 23(1):1-6. |
He Peiru, Dong Puhui, Song Xiyue, et al. Study on the thermo-sensitivity of thermo-sensitive male-sterile wheat line A3314[J]. Journal of Triticeae Crops, 2003, 23(1):1-6. | |
[21] |
Chen X D, Sun D F, Rong D F, et al. A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)[J]. Journal of Zhejiang University Science B, 2011, 12(11):943-950.
doi: 10.1631/jzus.B1000371 |
[22] | 何瑞锋, 章志宏. 大麦光温敏雄性不育系的基本特性研究[J]. 武汉大学学报(理学版), 2000, 46(4):492-494. |
He Ruifeng, Zhang Zhihong. Studies on the basic characteristics of photo-thermo-sensitive male sterile line of barley[J]. Journal of Wuhan University (Natural Science Edition), 2000, 46(4):492-494. | |
[23] | 王晓明, 宋国亮, 冯小磊, 等. 谷子光温敏雄性不育系A2高产繁育技术研究[J]. 河北农业科学, 2016, 20(2):5-9. |
Wang Xiaoming, Song Guoliang, Feng Xiaolei, et al. Research of high yield propagation technique for photo-thermo-sensitive genic male sterility of foxtail millet[J]. Journal of Hebei Agricultural Sciences, 2016, 20(2):5-9. | |
[24] | 马晓娣, 王建书, 卢彦琦, 等. 不同温度条件下高粱温敏雄性不育系冀130A育性变化规律及花粉败育研究[J]. 植物遗传资源学报, 2012, 13(2):212-218. |
Ma Xiaodi, Wang Jianshu, Lu Yanqi, et al. Characterization of fertility and pollen abortion of sorghum sterile line Ji 130A under different temperature conditions[J]. Journal of Plant Genetic Resources, 2012, 13(2):212-218. | |
[25] | 周筱妍, 白孟斌, 张传利, 等. 基于RAPD的SCAR标记初步定位油菜温敏雄性不育系K121S的育性转换基因[J]. 分子植物育种, 2016, 13(1):141-146. |
Zhou Xiaoyan, Bai Mengbin, Zhang Chuanli, et al. Molecular mapping of the fertility-conversion gene in rape thermo-sensitive genic male sterile line K121S (Brassica juncea L.) by SCAR marker based on RAPD[J]. Molecular Plant Breeding, 2016, 13(1):141-146. | |
[26] |
Li Bing, Chen Xueping, Wu Yanrong, et al. Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant (Solanum melongena L.)[J]. Horticulture Research, 2019, 6(1):1-16.
doi: 10.1038/s41438-018-0066-6 |
[27] | 付志远, 秦永田, 汤继华. 主要作物光温敏核雄性不育基因的研究进展与应用[J]. 中国生物工程杂志, 2018, 38(1):115-125. |
Fu Zhiyuan, Qin Yongtian, Tang Jihua. Reviews of photo-/thermo-sensitive genic male sterile gene in major crops[J]. China Biotechnology, 2018, 38(1):115-125. | |
[28] | 洪继仁, 杨素珍, 沈继丕. 高温影响棉花蕾铃生长的初步观察[J]. 上海农业科技, 1981(3):14-16. |
Hong Jiren, Yang Suzhen, Shen Jipi. Preliminary observation on the effect of high temperature on the growth of cotton bud and boll[J]. Shanghai Agricultural Science and Technology, 1981(3):14-16. | |
[29] | 唐海明, 余筱南. 棉花温敏雄性不育系特棉S-1的研究[J]. 中国棉花, 2005, 32(2):18-19. |
Tang Haiming, Yu Xiaonan. Study on Temian S-1, a temperature-sensitive male sterile line[J]. China Cotton, 2005, 32(2):18-19. | |
[30] | 周斌, 田月恒, 何觉民. 棉花温敏不育系TMS-2在湛江制种的可行性研究[J]. 湖南农业大学学报(自然科学版), 2007, 33(4):407-411. |
Zhou Bin, Tian Yueheng, He Juemin. Feasibility of crossbreeding of cotton thermo-sensitive male sterile line TMS-2 in Zhanjiang[J]. Journal of Hunan Agricultural University (Natural Sciences), 2007, 33(4):407-411. | |
[31] | 邵圣才, 刘辉. 棉花温敏雄性不育两用系研究与利用[J]. 中国棉花, 2000, 27(12):18-20. |
Shao Shengcai, Liu Hui. Research and utilization to cotton temperature-sensitive male sterile[J]. China Cotton, 2000, 27(12):18-20. | |
[32] | 刘冬梅, 周步进, 靳明凯, 等. 陆地棉‘洞A’育性研究时期的选择与花蕾外部形态指标间的关系[J]. 天津农业科学, 2013, 19(6):1-4. |
Liu Dongmei, Zhou Bujin, Jin Mingkai, et al. Relationship between period of anther development and bud diameter in upland cotton male sterility line ‘Dong A’[J]. Tianjin Agricultural Sciences, 2013, 19(6):1-4. | |
[33] | 宇文小岗, 王淑霞, 宇文璞, 等. 棉花雄性核不育系光温A系简介[J]. 中国棉花, 2006, 33(11):18. |
Yuwen Xiaogang, Wang Shuxia, Yuwen Pu, et al. Brief introduction of male male sterile line Guang-Wen A in cotton[J]. China Cotton, 2006, 33(11):18. | |
[34] | 曾潜, 戴恺, 何叔军, 等. 棉花光温敏雄性不育系湘QB研究初报[C]// 中国棉花学会. 中国棉花学会2013年年会论文汇编. 安阳: 中国棉花杂志社, 2013: 92-96. |
Zeng Qian, Dai Kai, He Shujun, et al. A preliminary study on male sterile cotton Xiang-QB with photo-thermo-sensitive[C]// China Society of Cotton Sci-Tech. Proceedings of the 2013 Annual Meeting of CSCS. Anyang: China Cotton Magazine House, 2013: 92-96. | |
[35] | 马建辉. 芽黄标记棉花光敏雄性不育系的选育及花药发育机理研究[D]. 杨凌: 西北农林科技大学, 2013. |
Ma Jianhui. The research of a novel photosensitive sterile cotton with virescent marker and unraveling molecular mechanism of anther development[D]. Yangling: Northwest A&F University, 2013. | |
[36] | 张朝军. 棉花光敏雄性核不育突变体PSM4创制及其特性研究[J]. 西北植物学报, 2018, 38(7):1212-1221. |
Zhang Chaojun. Research on creation and characteristics of photoperiod sensitive genetic male sterility mutant PSM4[J]. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(7):1212-1221. | |
[37] | 梁满中, 李训贞. 棉花花粉人工萌发及授粉液在杂交制种中的应用[J]. 湖南师范大学自然科学学报, 1998, 21(4):71-75. |
Liang Manzhong, Li Xunzhen. Artificial germination of Gossypium pollen and the utilization of pollinating solution in the hybrid seed production[J]. Journal of Natural Science of Hunan Normal University, 1998, 21(4):71-75. |
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