GhMADS13, whose primer designed according to submitted sequence, was obtained by PCR with the 100% identity to explore its function. qRT-PCR in different tissues revealed that GhMADS13 had the highest expression level in the flower, and this was hundreds of times greater than root with the lowest expression level. GhMADS13 was expressed in all floral organs, and expression levels in the sepal, petal, stamen, carpel, ovule were little different, and ovule had the highest expression level. GhMADS13 was inserted into a pBI121 vector, and a plant over-expression vector was constructed successfully. Two lines of transgenic Arabidopsis were obtained by floral-dipping. The results of the molecular detection and phenotypic data showed that the Arabidopsis plants with high expression levels of GhMADS13 were shorter, and produced less seeds and siliques. Based on the qRT-PCR results and the analysis of ectopic expression, we deduce GhMADS13 mainly negatively regulates ovule development.
JIANG Su-Cheng, SONG Mei-Zhen, PANG Chao-You, WEI Heng-Ling, FAN Shu-Li, YU Shu-Xun.
Analysis of Transgenic Arabidopsis thaliana with the Gossypium hirsutum L. MADS- box Gene GhMADS13 [J]. Cotton Science, 2013, 25(5): 377-381. https://doi.org/10.11963/cs130501
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参考文献
[1] MESSENGUY F，Dubois E．Role of MADS box proteins and their cofactors in combinatorial control of gene expression and cell development [J］．Gene，2003，316(1): 1-21．
[2] COEN E S，Meyerowitz E M．The war of the whorls: genetic interactions controlling flower development [J］．Nature，1991，353(6339): 31-37．
[3] SCHWARZ-SOMMER Z，Huijser P，Nacken W，et al．Genetic control of flower development by homeotic genes in Antirrhinum majus [J］．Science，1990，250(4983): 931-936．
[4] BOWMAN J L，Smyth D R，Meyerowitz E M．Genetic interactions among floral homeotic genes of Arabidopsis [J］．Development，1991，112(1): 1-20．
[5] WEIGEL D，Meyerowitz E M．The ABCs of floral homeotic genes [J］．Cell，1994，78(2): 203-209．
[6] ANGENENT G C，Colombo L．Molecular control of ovule development [J］．Trends in Plant Science，1996，1(7): 228-232．
[7] PELAZ S，Ditta G S，Baumann E，et al．B and C floral organ identity functions require SEPALLATA MADS-box genes [J］．Nature，2000，405(6783): 200-203．
[8] THEIBEN G．Development of floral organ identity: stories from the MADS house [J］． Current Opinion in Plant Biology，2001，4(1): 75-85．
[9] 吴 东，喻树迅，范术丽，等．棉花 MADS-box 蛋白基因 (GhMADS-13) 的克隆和表达分析 [J］． 基因组学与应用生物学，2009，28(2)：223-228． WU Dong，Yu Shu-xun，Fan Shu-li，et al．Cloning and expression analysis of a MADS-box protein gene (GhMADS-13) from upland cotton [J］．Genomics and Applied Biology，2009，28(2): 223-228．
[10] MA H，Yanofsky M F，Meyerowitz E M．AGL1-AGL6，an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes [J］．Genes & Development，1991，5(3): 484-495．
[11] ALVAREZ-Buylla E R，Pelaz S，Liljegren S J，et al．An ancestral MADS-box gene duplication occurred before the divergence of plants and animals [J］．Proceedings of the National Academy of Sciences of USA，2000，97(10): 5328-5333．
[12] CLOUGH S J，Bent A F．Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis tha- liana [J］．The Plant Journal，1998，16(6): 735-743．
[13] KOO S C，Bracko O，Park M S，et al．Control of lateral organ development and flowering time by the Arabidopsis thaliana MADS‐box Gene AGAMOUS‐LIKE6 [J］．The Plant Journal，2010，62(5): 807-816．
[14] HSU H F，Huang C H，Chou L T，et al．Ectopic expression of an orchid (Oncidium Gower Ramsey) AGL6-like gene promotes flowering by activating flowering time genes in Arabidopsis thaliana [J］．Plant and Cell Physiology，2003，44(8): 783-794．
[15] FAN Jin-hui，Li Wen-qiong，Dong Xin-chun，et al．Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis [J］．Science in China Series C: Life Sciences，2007，50(5): 676-689．
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