Somatic Embryogenesis and Regeneration of Green Cotton(Gossypium hirsutum L.) Xincaimian No.7
ZHU Hua-guo*, WANG Hong-juan*, LI Peng-fei, ZHANG Xin-yu, LI Yan-jun, SUN Jie**
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College of Agriculture /The Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Group, Shihezi University, Shihezi, Xinjiang 832003，China
Using hypocotyls and cotyledon of Xincaimian No.7(Green fiber cotton cultivar) as explants, MSB(MS salts and B5 vitamins) medium supplemented with different phytohormone combinations as callus induction media, and by embryogenic callus differentiation, regeneration experiment was carried out. The results indicated that the calli were easily induced in medium supplemented with 0.1 mg·L-1 2，4-D and 0.1 mg·L-1 KT, the frequency of callus induction was 100%. Split hypocotyl segments reversely cultured on medium was the best mode for callus induction. 0.3 mg·L-1 IBA and 0.15 mg·L-1 KT was the best phytohormone combination for embryogenic callus differentiation, and the highest differentiation frequency was 23.33%. Somatic embryos were acquired in MSB medium with NH4NO3 free and doubled KNO3, supplemented with 0.5 g·L-1 Asn and 1 g·L-1 Gln. Complete cotyledon embryos were isolated and cultured on hormone-free 1/2MS medium for germination and development. The regeneration protocol of Xincaimian No.7 via somatic embryogenesis was established, laying a solid foundation for gene engineering in naturally colored cotton.
ZHU Hua-Guo, WANG Hong-Juan, LI Peng-Fei, ZHANG Xin-Yu, LI Yan-Jun, SUN Jie.
Somatic Embryogenesis and Regeneration of Green Cotton(Gossypium hirsutum L.) Xincaimian No.7[J]. Cotton Science, 2013, 25(2): 110-114. https://doi.org/10.11963/cs130203
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