不同磷效率棉花根系形态和磷酸酶活性对供磷强度的响应

doi:10.11963/1002-7807.zmfg.20170329

摘要/Abstract

摘要： 目的研究不同磷效率棉花品种的根系形态和磷酸酶活性对不同供磷强度的响应。方法以前期筛选获得的磷高效品种新陆早19号和磷低效品种新陆早13号为材料,通过营养液培养比较不同供磷强度下各品种生物量、磷含量、根系形态和磷酸酶活性的变化。结果供磷强度达到50 μmol·L^-1后,棉花生物量和根系长度不再随供磷强度增加而增加。棉花根表分泌磷酸酶活性随供磷强度增加而急剧下降,营养液实际pH下根系分泌的酸性磷酸酶活性与植株磷浓度显著负相关。根系形态对供磷强度的响应不敏感,仅在不供磷与供磷条件下存在显著差异。2个品种在磷效率方面的差异主要表现在磷的利用效率上,在供磷强度为0~250 μmol·L^-1条件下,新陆早19号的磷利用效率显著高于新陆早13号。结论棉花根系形态特征参数和根系分泌质子与有机酸的数量对供磷强度的响应不敏感。不同磷利用效率品种的发现对于今后棉花节磷增效的生物学潜力与调控途径的研究提供了十分有效的实验材料。

关键词: 棉花; 供磷强度; 磷酸酶; 根系形态; 磷效率

Abstract:

[Object] To understand the variation of the response of root morphology and phosphatase activity of two cotton varieties to five phosphorus supply levels. [Method] A high phosphorus efficient variety Xinluzao 19 and a low phosphorus efficient variety Xinluzao 13 were selected as testing plants, through comparing the changes of biomass, phosphorus content, root morphology and phosphatase activity at different phosphorus supply levels in nutrient solution culture. [Result] When phosphorus supply was higher than 50 μmol·L^-1, the increase of phosphorus supply will not improve biomass and root length anymore. The activity of phosphatase on cotton root surface decreased sharply with the increase of phosphorus supply, the activity of acid phosphatase at actual pH was negatively correlated with plant phosphorus concentration. The response of root morphology to phosphorus supply was not sensitive, there was a significant difference only under the conditions between treatment of no phosphorus and those of phosphorus supply. When phosphorus supply was 0~250 μmol·L^-1, the P utilization efficiency of Xinluzao 19 was significantly higher than that of Xinluzao 13. [Conclusion] It can be considered that the response of cotton root morphology characteristic parameters and the quantity of the root release of protons and organic acids to phosphorus supply is not sensitive. This provided a very effective experimental material on saving phosphorus and increasing its efficiency of the biological potential and regulation pathway of cotton with phosphorus utilization efficiency genotypes discovered.

Key words: cotton; phosphorus supply; phosphatase; root morphology; phosphorus efficiency

张敏,盛建东,白灯莎·买买提艾力,冯固. 不同磷效率棉花根系形态和磷酸酶活性对供磷强度的响应[J]. 棉花学报, 2017, 29(3): 283-291.

Min Zhang,Jiandong Sheng,Maimaitiaili Baidengsha,Gu Feng. Response of Root Morphology and Phosphatase Activity of Cotton to Phosphorus Supply[J]. Cotton Science, 2017, 29(3): 283-291.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.zmfg.20170329

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2017/V29/I3/283

参考文献

[1]	Smil V.Phosphorus in the environment: Natural flows and human interferences[J]. Annual Review of Energy and the Environment, 2000, 25(1): 53-88.
[2]	沈善敏. 论我国磷肥的生产与应用对策(一)[J]. 土壤通报, 1985, 16(3): 97-103.
[2]	Shen Shanmin.Production and application of phosphate fertilizer in China, Ⅰ[J]. Chinese Journal of Soil Science, 1985, 16(3): 97-103.
[3]	王庆仁, 李继云, 李振声. 高效利用土壤磷素的植物营养学研究[J]. 生态学报, 1999, 19(3): 417-421.
[3]	Wang Qingren, Li Jiyun, Li Zhensheng.Plant nutrition research of high efficient utilization of soil phosphorus[J]. Journal of Ecology, 1999, 19(3): 417-421.
[4]	严小龙, 戈振扬. 植物根构型特性与磷吸收效率[J]. 植物学通报, 2000, 17(6): 511-519.
[4]	Yan Xiaolong, Ge Zhenyang.Characteristics of plant root architecture and phosphorus uptake efficiency[J]. Chinese Bulletin of Botany, 2000, 17(6): 511-519.
[5]	李锋, 潘晓华, 刘水英, 等. 低磷胁迫对不同水稻品种根系形态和养分吸收的影响[J]. 作物学报, 2004, 30(5): 438-442.
[5]	Li Feng, Pan Xiaohua, Liu Shuiying, et al.Effect of phosphorus deficiency stress on root morphology and nutrient absorption of rice cultivars[J]. Journal of Crop Science, 2004, 30(5): 438-442.
[6]	王文华, 范婷婷, 罗兰艳, 等. 甘蓝型油菜不同磷效率品种根系形态及生理特性的研究[J]. 中国土壤与肥料, 2011(4): 26-29.
[6]	Wang Wenhua, Fan Tingting, Luo Lanyan, et al.Studies on root morphology and physiological characteristics of different phosphorus efficiency cultivars in Brassica[J]. Soil and Fertilizer in China, 2011(4): 26-29.
[7]	周丽莉. 蚕豆、大豆、玉米根系质子和有机酸分泌差异及其在间作磷营养中的意义[D]. 北京: 中国农业大学, 2005.
[7]	Zhou Lili.Broad bean, soybean, maize root system proton and organic acid secretion and its significance in the inter phosphorus nutrition[D]. Beijing: China Agricultural University, 2005.
[8]	王悦, 符力, 冯固. 根际pH对玉米利用磷酸单酯和生长盐的影响[J]. 植物营养与肥料学报, 2014, 20(4): 870-876.
[8]	Wang Yue, Fu Li, Feng Gu.Effects of rhizosphere pH on utilization of P in phytin and lecithin by maize[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(4): 870-876.
[9]	田中民, 王晨. 缺磷白羽扇豆排根与非排根区根尖分泌有机酸的比较[J]. 植物生理学报, 2000, 26(4): 317-322.
[9]	Tian Zhongmin, Wang Chen.Compare white lupin cluster root with non-cluster root zone exudates organic on phosphorus deficiency[J]. Plant Physiology, 2000, 26(4): 317-322.
[10]	Wang X, Tang C, Guppy C N, et al.Cotton, wheat and white lupin differ in phosphorus acquisition from sparingly soluble sources[J]. Environmental and Experimental Botany, 2010, 69(3): 267-272.
[11]	Wang X, Tang C, Guppy C N, et al.Phosphorus acquisition characteristics of cotton (Gossypium hirsutum L.), wheat(Triticum aestivum L.) and white lupin(Lupinus albus L.) under P deficient conditions[J]. Plant and Soil, 2008, 312(1/2): 117-128.
[12]	李卫华. 棉花磷素高效利用品种筛选及机理的初步研究[D].乌鲁木齐: 新疆农业大学, 2010.
[12]	Li Weihua.Research on phosphorus efficient genotypes of cotton varieties screening and its mechanism[D]. Urumqi: Xinjiang Agricultural University, 2010.
[13]	王靓, 盛建东, 罗佳, 等. 不同基因型棉花磷效率的差异特征[J]. 中国土壤与肥料, 2015(3): 44-49.
[13]	Wang Liang, Sheng Jiandong, Luo Jia, et al.Differential characteristics of phosphorus efficiency in different genotypes of cotton[J]. Soil and Fertilizer in China, 2015(3): 44-49.
[14]	张炎, 崔水利, 王讲利, 等. 不同施磷量对棉花根系形态和磷吸收的影响[J]. 新疆农业科学, 1996(2):82-84.
[14]	Zhang Yan, Cui Shuili, Wang Jiangli, et al.Effects of different phosphorus levels on root morphology and phosphorus uptake of cotton[J]. Xinjiang Agricultural Sciences, 1996(2): 82-84.
[15]	何丹, 张锡洲, 李廷轩, 等. 稻种资源的磷利用效率差异及其分类评价[J]. 中国土壤与肥料, 2012(3): 70-76.
[15]	He Dan, Zhang Xizhou, Li Tingxuan, et al.The efficiency difference and classification evaluation of rice germplasm resources using phosphorus[J]. Soil and Fertilizer in China, 2012(3): 70-76.
[16]	刘庆花. 玉米根际微生态环境与磷素高效利用的研究[D]. 长春: 吉林农业大学, 2003.
[16]	Liu Qinghua.Study on the rhizosphere micro ecological environment and high utilization efficiency of phosphorus in Maize [D]. Changchun: Jilin Agricultural University, 2003.
[17]	Vance C P, Uhde-Stone C, Allan D L.Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource[J]. New Phytologist, 2003, 157(3): 423-447.
[18]	Tadano T, Sakai H.Secretion of acid phosphatase by the roots of several crop species under phosphorus-deficient conditions[J]. Soil Science and Plant Nutrition, 1991, 37(1): 129-140.
[19]	Goldstein A H, Baertlein D A, Mcdaniel R G.Phosphate Starvation Inducible Metabolism in Lycopersicon esculentum[J]. Plant Physiol, 1988, 87(3): 711-715.
[20]	孙海国. 不同基因型小麦适应缺磷胁迫的根系形态及生理特征研究[D]. 北京: 中国农业大学, 1999.
[20]	Sun Haiguo.Morphological and physiological changes of root system of wheat genotypes under phosphorus deficiency[D].Beijing: China Agricultural University, 1999.
[21]	沈宏, 施卫明, 王校常, 等. 不同作物对低磷胁迫的适应机理研究[J]. 植物营养与肥料学报, 2001, 7(2): 172-177.
[21]	Shen Hong, Shi Weiming, Wang Xiaochang, et al.Study on adaptation mechanism of different crops to low phosphorus stress[J]. Journal of Plant Nutrition and Fertilizer, 2001, 7(2): 172-177.
[22]	郭程瑾, 李宾兴, 周彦珍, 等. 不同磷效率小麦品种的磷吸收特性[J]. 植物遗传资源学报, 2006, 7(1): 49-53.
[22]	Guo Chengjin, Li Binxing, Zhou Yanzhen, et al.Phosphorus uptake characteristics of wheat varieties with different phosphorus efficiency[J]. Journal of Plant Genetic Resources, 2006, 7(1): 49-53.
[23]	阳显斌, 张锡洲, 李廷轩, 等. 小麦磷素利用效率的品种差异[J]. 应用生态学报, 2012, 23(1): 60-66.
[23]	Yang Xianbin, Zhang Xizhou, Li Tingxuan, et al.Variety difference of phosphorus use efficiency of wheat[J]. Journal of Applied Ecology, 2012, 23(1): 60-66.
[24]	李宾兴, 郭程瑾, 王斌, 等. 缺磷胁迫下不同磷效率小麦品种及其杂种F1的磷吸收利用特性[J]. 作物学报, 2006, 32(2): 267-272.
[24]	Li Binxing, Guo Chengjin, Wang Bin, et al.Phosphorus uptake and utilization characteristics of wheat cultivars with different phosphorus efficiency under phosphorus deficiency stress and their hybrid F1[J]. Journal of Crop Science, 2006, 32(2): 267-272.