陆地棉抗旱性与SSR分子标记的关联分析

doi:10.11963/1002-7807.sxhwhm.20170407

摘要/Abstract

摘要： 目的了解陆地棉抗旱性和寻找与陆地棉抗旱性相关的分子标记,挖掘与抗旱性状相关的优异等位变异。方法以191份陆地棉栽培种组成的自然群体为材料,在萌发期采用质量分数为15%的PEG6000竖直滤纸法对191份材料进行胁迫处理,测定其发芽率。根据隶属函数值对萌发期抗旱性划分抗旱级别。利用关联分析的方法以74对SSR引物对该自然群体进行基因组变异扫描。在利用Structure 2.3.4分析该自然群体的遗传结构的基础上,使用Tassel 2.0的GLM模型将基因型数据与萌发期抗旱性状进行关联分析,寻找与抗旱性状相关的分子标记。结果筛选出强抗旱材料46份,中等抗旱材料59份,干旱敏感材料86份;群体结构分析将191份材料划分为2个亚群,分别包含123份和68份材料;通过关联分析获得与陆地棉萌发期抗旱性显著相关的分子标记15个,各位点对表型变异解释率为2.2%~7.49%。结论本研究筛选出强抗旱材料46份,获得与萌发期抗旱性相关的分子标记15个,可供棉花抗旱标记辅助选择育种利用。

关键词: 陆地棉; SSR; 关联分析; 抗旱性

Abstract:

[Objective] The aims of this study are to detect drought tolerance of upland cotton (Gossypium hirsutum L.), to find the molecular markers associated with the drought tolerance traits, and to excavate elite allelic variation associated with drought resistance traits. [Method] We selected 191 cultivars (lines) as experimental materials. The germination rates during sprouting period were evaluated in 15% (mass fraction) PEG6000 stress. Drought tolerance was classified according to the membership function values to drought resistance at sprouting period. A total of 74 pairs of simple sequence repeat (SSR) primers were employed to detect the genomic variation of the natural population. Based on the analysis of the genetic structure of the natural population using Structure 2.3.4, the GLM model of Tassel 2.0 was used to correlate the genotype data with the drought tolerance traits at germination stage to find the molecular markers associated with the drought tolerance traits. [Result] A total of 46 high-resistance materials, 59 medium-drought resistant materials and 86 drought-sensitive materials were identified; population structure analysis divided the 191 materials into two subgroups, consisting of 123 and 68 materials, respectively; the association analysis showed that 15 SSR sites related to drought resistance at germination stage (P < 0.05), explained phenotypic variation ranged from 2.2% to 7.49%. [Conclusion] We screened out 46 drought-resistant materials and 15 molecular markers related to drought tolerance, which may be used for cotton drought-resistant marker-assisted breeding.

Key words: upland cotton; simple sequence repeat; association analysis; drought-resistance

桑晓慧,赵云雷,王红梅,陈伟,龚海燕,赵佩,崔艳利. 陆地棉抗旱性与SSR分子标记的关联分析[J]. 棉花学报, 2017, 29(3): 241-252.

Xiaohui Sang,Yunlei Zhao,Hongmei Wang,Wei Chen,Haiyan Gong,Pei Zhao,Yanli Cui. Association Analysis of Drought Tolerance and SSR Markers in Upland Cotton[J]. Cotton Science, 2017, 29(3): 241-252.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.sxhwhm.20170407

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2017/V29/I3/241

参考文献

[1]	Ashraf M.Inducing drought tolerance in plants: Recent advance[J]. Biotechnology Advances, 2010, 28(1): 169-183.
[2]	王延琴, 杨伟华, 许红霞, 等. 水分胁迫对棉花种子萌发的影响[J]. 棉花学报, 2009, 21(1): 73-76.
[2]	Wang Yanqin, Yang Weihua, Xu Hongxia, et al.Effect of water stress on germination of cotton seeds[J]. Cotton Science, 2009, 21(1): 73-76.
[3]	牛瑞明, 王燕, 吴桂丽, 等. 裸燕麦种子萌发对模拟干旱胁迫的响应及其耐旱性综合评价[J]. 麦类作物学报, 2011, 31(4): 753-756.
[3]	Niu Ruiming, Wang Yan, Wu Guili, et al.Evaluation of drought resistant and response of naked oats seed germination under drought stress[J]. Journal of Triticeae Crops, 2011, 31(4): 753-756.
[4]	吴永美, 吕炯章, 王书建, 等. 植物抗旱生理生态特性研究进展[J]. 杂粮作物, 2008, 28(2): 90-93.
[4]	Wu Yongmei, Lü Jiongzhang, Wang Shujian, et al.Research progress on physiological and ecological characteristics of plant drought resistance[J]. Rain Fed Crops, 2008, 28(2): 90-93.
[5]	Flint-Garcia S A, Thomsberry J M, Buckler E S. Structure of linkage disequilibrium in plants[J]. Annual Review of Plant Biology, 2003, 54: 357-374.
[6]	Flint-Garcia S A, Thuillet A C, Yu J M, et al. Maize association population: a high-resolution platform for quantitative trait locus dissection[J]. The Plant Journal, 2005, 44: 1054-1064.
[7]	Yu J M, Buckler E S.Genetic association mapping and genome organization of maize[J]. Current Opinion in Biotechnology, 2006, 17(2): 155-160.
[8]	曹士亮, 曹靖生, 张建国, 等. 基于SSR标记的玉米丝黑穗病抗性的关联分析[J]. 玉米科学, 2012, 20(4): 126-130.
[8]	Cao Shiliang, Cao Jingsheng, Zhang Jianguo, et al.Correlation analysis of host resistance to head sumt in maize based on SSR markers[J]. Journal of Maize Sciences, 2012, 20(4): 126-130.
[9]	张嘉楠. 小麦资源材料抗旱相关性状关联分析及优异等位基因发掘[D]. 保定: 河北农业大学, 2010.
[9]	Zhang Jianan.Association mapping and elite allele tapping for traits related to drought resistance in wheat (Triticum aestivum L.)[D]. Baoding: Agricultural University of Hebei Province, 2010.
[10]	李兆南. 大豆抗旱、耐盐性鉴定及其与SSR标记的关联分析[D]. 长春: 吉林大学, 2011.
[10]	Li Zhaonan.Association analysis of genotyping data uncovered strong relationship between draught, salt tolerance identification in collected soybean (Glycine max) cultivars and selected SSR markers[D]. Changchun: Jilin University, 2011.
[11]	黎东华, 刘文萍, 张艳欣, 等. 芝麻耐旱性的鉴定方法及关联分析[J]. 作物学报, 2013, 39(8): 1425-1433.
[11]	Li Donghua, Liu Wenping, Zhang Yanxin, et al.Identification method of drought tolerance and association mapping for sesame (Sesamum indicum L.)[J]. Acta Agronomica Sinica, 2013, 39(8): 1425-1433.
[12]	Zhao Yunlei, Wang Hongmei, Chen Wei, et al. Genetic structure, linkage disequilibrium and association mapping of Verticillium wilt resistance in elite cotton (Gossypium hirsutum L.) germplasm population[J/OL]. PLoS ONE, 2014, 9(1): e86308 [2017-02-15]. .
[13]	王俊娟. 陆地棉种质资源的抗旱性评价及遗传研究[D]. 北京: 中国农业科学院, 2011.
[13]	Wang Junjuan.Drought-resistance evaluation and its genetic research on upland cotton[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011.
[14]	邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2006.
[14]	Qiu Lijuan, Chang Ruzhen.Descriptors and data standard for soybean (Glycine spp.)[M]. Beijing: China Agriculture Press, 2006.
[15]	兰巨生. 农作物综合抗旱性评价方法的研究[J]. 西北农业学报, 1998, 7(3): 85-87.
[15]	Lan Jusheng.Comparison of evaluating methods for agronomic drought resistance in crops[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 1998, 7(3): 85-87.
[16]	王曙光, 孙黛珍, 周福平, 等. 六倍体小黑麦萌发期抗旱性分析[J]. 中国生态农业学报, 2008, 16(6): 1403-1408.
[16]	Wang Shuguang, Sun Daizhen, Zhou Fuping, et al.Analysis of drought resistance of hexaploid triticale at sprouting stage[J]. Chinese Journal of Eco-Agriculture, 2008, 16(6): 1403-1408.
[17]	Parerson A H, Brubaker C L, Wendel J F.A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis[J]. Plant Mol Bio Rep, 1993, 11(2): 122-127.
[18]	张军, 张天真, 武耀廷, 等. 棉花微卫星标记的 PAGE / 银染快速检测[J]. 棉花学报, 2000, 12(5): 267-269.
[18]	Zhang Jun, Zhang Tianzhen, Wu Yaoting, et al.Fast screening of microsatellite markers in cotton with PAGE/silver staining[J]. Cotton Science, 2000, 12(5): 267-269.
[19]	Liu K J, Muse S V.Power marker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21: 2128-2129.
[20]	Pritchard J K, Stephens M, Donnelly P.Inference of population structure using multilocus genotype data[J]. Genetics, 2000, 155: 945-959.
[21]	Bradbury P J, Zhang Z, Kroom D E, et al.TASSEL: software for association mapping of complex traits in diverse samples[J]. Bioinformatics, 2007, 23: 2633-2635.
[22]	Evanno G, Regnaut S, Goudet J.Detecting the number of clusters of individuals using the software structure-a simulation study[J]. Molecular Ecology, 2005, 14: 2611-2620.
[23]	贾继增. 分子标记种质资源鉴定和分子标记育种[J]. 中国农业科学, 1996, 29(4): 1-10.
[23]	Jia Jizeng.Molecular germplasm diagnostics and molecular marker-assisted breeding[J]. Scientia Agricultura Sinica, 1996, 29(4): 1-10.
[24]	周奕华, 陈正华. 分子标记在植物学中的应用及前景[J]. 武汉植物学研究, 1999, 17(1): 75-86.
[24]	Zhou Yihua, Chen Zhenghua.Development and application of molecular markers in botany[J]. Journal of Wuhan Botanical Research, 1999, 17(1): 75-86.
[25]	Jia Yinhua, Sun Junling, Wang Xiwen, et al.Molecular diversity and association analysis of drought and salt tolerance in Gossypium hirsutum L. germplasm[J]. Journal of Integrative Agriculture, 2014, 13(9): 1845-1853.
[26]	Camus-Kulandaivelu L, Veyrieras J B, Madur D, et al.Maize adaptation to temperate climate: relationship between population structure and polymorphism in the Dwarf8 gene[J]. Genetics, 2006, 172(4): 2449-2463.
[27]	文自翔, 赵团结, 郑永战, 等. 中国栽培和野生大豆农艺品质性状与SSR标记的关联分析: Ι.群体结构及关联标记[J]. 作物学报, 2008, 34(7): 1169-1178.
[27]	Wen Zixiang, Zhao Tuanjie, Zheng Yongzhan, et al.Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China: Ι. Population structure and associated markers[J]. Acta Agronomica Sinica, 2008, 34(7): 1169-1178.