昼夜变温下高温对Bt棉铃壳杀虫蛋白及氮代谢生理的影响

doi:10.11963/1002-7807.lycdh.20180102

摘要/Abstract

摘要： 【目的】确定在昼夜变温条件下，高温逆境对转基因棉花铃壳中杀虫蛋白含量的影响及相关生理特征。[方法]以大面积推广应用的Bt棉常规品种泗抗1号和杂交种泗抗3号为材料，于2011―2012年在扬州大学遗传生理重点实验室，用盆栽棉花在人工气候室设计昼夜变温（39/27 ℃）条件下高温持续4～10 d胁迫，研究铃壳中Bt蛋白含量、氮代谢相关物质和关键酶活性的变化特征。【结果】铃壳中Bt杀虫蛋白含量随胁迫时间的延长而降低。与对照（32/25 ℃）相比，胁迫7 d后铃壳中Bt杀虫蛋白开始急剧下降。2011年度常规种泗抗1号和杂交种泗抗3号分别下降了18.44%和14.82%；2012年分别下降了19.07%和15.26%。铃壳中Bt杀虫蛋白下降同时，其游离性氨基酸含量和蛋白酶活性明显上升，而可溶性蛋白含量和GPT活性则显著下降。【结论】昼夜变温下高温引起铃壳中蛋白质合成减弱，分解加剧，导致蛋白质含量下降（包括Bt蛋白含量下降），从而引起抗虫性降低。

关键词: Bt棉; 高温胁迫; Bt杀虫蛋白; 氮代谢; 铃壳

Abstract: [Object] In this study, the objective was to study changes in the boll wall's Bt protein concentration and related physiological characteristics under high alternating day and night temperature conditions in Bt cotton. [Method] The experiments were conducted at the Key Laboratory of Genetics and Physiology, Yangzhou University during the 2011-2012 cotton growing seasons. The conventional Bt cotton cultivar Sikang-1 and hybrid cultivar Sikang-3 were used as the test materials. The boll wall's Bt protein content and nitrogen metabolic physiology were investigated under high temperatures (39/27 ℃, day/night) for 4-10 d at the peak boll stage. [Result] The Bt protein content in the boll wall decreased as the stress time increased. Compared with the control (day/night, 32/25 ℃), the Bt protein content in the boll wall decreased significantly at 7 d under the high temperature stress. The values for cultivars Sikang-1 and Sikang-3 decreased by 18.44% and 14.82%, respectively, in 2011, and by 19.07% and 15.26%, respectively, in 2012. As the Bt toxin content decreased, the boll wall's free amino acid content and protease activity increased, while the soluble protein content and GPT (Glutamic-pyruvic transaminase) activity decreased significantly. [Conclusion] The reduction in Bt protein synthesis and increase in the insecticidal protein degradation at the boll wall under high temperatures caused a reduction in the protein content, including the Bt protein content, which resulted in a reduction in insect resistance.

Key words: Bt cotton; high temperature stress; Bt protein; nitrogen metabolism; boll wall

中图分类号:

S562.01

李远, 王桂霞, 胡大鹏, Abidallah Eltayib, 张丽雅, 张祥, 陈源, 陈德华. 昼夜变温下高温对Bt棉铃壳杀虫蛋白及氮代谢生理的影响[J]. 棉花学报, 2018, 30(1): 38-44.

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