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棉花学报 ›› 2018, Vol. 30 ›› Issue (3): 252-260.doi: 10.11963/1002-7807.lsyzxh.20180409

• 研究与进展 • 上一篇    下一篇

外源24-表油菜素内酯对低温胁迫下棉花幼苗光合生理的影响

李淑叶,马慧娟,张思平,刘绍东,沈倩,陈静,葛常伟,庞朝友*,赵新华*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室, 河南 安阳 455000
  • 收稿日期:2017-09-28 出版日期:2018-05-15 发布日期:2018-05-15
  • 通讯作者: 庞朝友,chypang@163.com;赵新华,zhaoxinhua1968@126.com
  • 作者简介:李淑叶(1990-),女,硕士研究生,15737295256@163.com
  • 基金资助:
    公益性行业(农业)科研专题:水浇地合理耕层构建技术指标研究(201503117);国家科技支撑计划“棉花高产高效关键技术研究与示范”(2014BAD11B02);国家现代农业产业技术体系建设专项资金(CARS-18-17)

Effects of Exogenous 24-epibrassinolide on Photosynthetic Physiology of CottonSeedlings under Low Temperature

Li Shuye, Ma Huijuan, Zhang Siping, Liu Shaodong, Shen Qian, Chen Jing, Ge Changwei, Pang Chaoyou*, Zhao Xinhua*   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 45500, China
  • Received:2017-09-28 Online:2018-05-15 Published:2018-05-15

摘要: 【目的】研究外源24-表油菜素内酯(24-Epibrassinolide, EBR)对低温胁迫下棉花幼苗光合生理的影响,为EBR作为生长调节剂提高棉花耐冷能力提供依据。【方法】以中棉所60、鲁棉研28和泗棉3号为试验材料,在中棉所试验农场东场(河南省安阳县)进行大田试验,棉花苗期第一次低温来临前叶面喷施蒸馏水(CK)和不同浓度的EBR(0.1 mg·L-1和0.2 mg·L-1),3 d后测定叶片的相对电导率、叶绿素含量和快速叶绿素荧光诱导动力学曲线(OJIP曲线)及荧光参数。【结果】低温胁迫下,中棉所60、鲁棉研28和泗棉3号喷施EBR后相对电导率较对照下降17.7%~32.8%,中棉所60和鲁棉研28不同浓度EBR处理没有显著差异,但泗棉3号0.2 mg·L-1 EBR处理较0.1 mg·L-1 EBR处理叶片相对电导率显著降低;棉花叶片喷施EBR后叶绿素a和叶绿素b含量较对照分别提高9.7%~32.6%和15.0%~18.9%,光系统Ⅱ(PhotosystemⅡ, PSⅡ)最大光化学效率(FV/FM )和基于吸收光能的性能指数(PIABS,Performance index on absorption basis)显著提高,其中中棉所60在0.1 mg·L-1 EBR处理后PIABS提高幅度最大为75.6%,鲁棉研28和泗棉3号喷施0.2 mg·L-1 EBR后PIABS增加幅度最大,分别提高101.1%和265.6%,单位受光面积吸收的光能(ABS/CSm)、单位有活性反应中心将电子传递到电子传递链QA下游其他电子受体的能量(ETo/RC)和将电子传递到QA下游电子受体的概率(φEo)显著提高。【结论】外源EBR可以降低低温胁迫下棉花幼苗的相对电导率,通过提高叶片光能捕获能力、光合电子传递能力和叶绿素含量缓解低温对棉花光合作用的抑制,其中中棉所60喷施0.1 mg·L-1 EBR处理效果较好,鲁棉研28和泗棉3号喷施0.2 mg·L-1处理效果较好。

关键词: 棉花; 低温; 24-表油菜素内酯; 叶绿素荧光

Abstract: [Objective] The objective of this study was to investigate the effects of exogenous 24-epibrassinolide (EBR) on the photosynthetic physiology of cotton seedlings under low temperature and to provide basis for improving the cold tolerance of cotton by using EBR as growth regulator. [Method] Taking CCRI 60, Lumianyan 28 and Simian 3 as materials, a field experiment was carried out in Institute of Cotton Research of CAAS(Anyang county, Henan province). Before the first low temperature treatment, the cotton seedlings were sprayed with distilled water (Control) and different concentrations of EBR (0.1 mg·L-1 and 0.2 mg·L-1), respectively. After 3 days, the relative electrical conductivity, chlorophyll content, rapid chlorophyll fluorescence induction kinetic curve (OJIP) and fluorescence parameters were measured. [Result] Under low temperature, the relative conductivity of CCRI 60, Lumianyan 28 and Simian 3 spraying with EBR decreased by 17.7%~32.8% compared with control, and there was no significant difference between CCRI 60 and Lumianyan 28 in different concentrations of EBR treatments, but the relative conductivity of Simian 3 treating with 0.2 mg·L-1 EBR was significantly lower than those treatments with 0.1 mg·L-1 EBR . The chl a and chl b contents increased by 9.7%~32.6% and 15.0%~18.9%, respectively. The maximum photochemical efficiency of photosystemⅡ (Fv/FM) and photosynthetic performance index on absorption basis(PIABS) increased significantly. PIABS of CCRI 60 increase the maximum by 75.6% using 0.1 mg·L-1 EBR. Lumianyan 28 and Simian 3 increased the maximum by 101.1% and 265.6% using 0.2 mg·L-1 EBR, respectively; Absorbed photon flux per cross section (ABS/CSm), electron transport flux (further than QA) per active reactive center (ETo/RC) and probability for electron transport (φEo) are significantly increased. [Conclusion] Exogenous EBR can enhance the ability of low temperature tolerance of cotton seedlings and alleviate the inhibition of photosynthesis in cotton at low temperature. The study showed that 0.1 mg·L-1 EBR performs well in CCRI 60 and 0.2 mg·L-1 in Lumianyan 28 and Simian 3.

Key words: Cotton; low temperature; 24-epibrassinolide; chlorophyll fluorescence induction kinetic curve

中图分类号: 
  • S562.01