不同处理诱导新海16号体细胞胚胎同步化发生

doi:10.11963/1002-7807.gjyqyy.20170531

摘要/Abstract

摘要： 【目的】旨在探索诱导海岛棉体细胞胚胎同步化发生的有效方法。【方法】以海岛棉品种新海16号的胚性愈伤组织为材料，研究了低温、饥饿和渗透处理诱导体细胞胚胎同步化发生的效果。【结果】在低温处理组中，4 ℃诱导时体细胞胚胎发生的数量随着低温诱导时间的延长而显著增加；10 ℃诱导时，不同诱导时间下各处理体细胞胚胎发生的数量差异不显著，但都显著高于对照（28 ℃）；缺磷、缺氮、缺肌醇3种饥饿处理的体细胞胚胎的数量都随着处理时间的延长而减少；渗透处理组中，在含40 g·L^－1葡萄糖的培养基上诱导7 d的效果最佳。【结论】4 ℃处理3 d、缺磷处理5 d、缺氮处理5 d以及40 g·L^－1葡萄糖诱导7 d可以显著促进新海16号的体细胞胚胎同步化发生，其中4 ℃处理3 d的诱导效果最佳。

关键词: 海岛棉; 体细胞胚胎; 诱导; 同步化

Abstract: [Objective] The aim of this study was to develop an efficient method for synchronizing the somatic embryogenesis of Gossypium barbadense L. [Method] We attempted to synchronize the somatic embryogenesis of G. barbadense L. cv. 'Xinhai 16' embryonic calli. The somatic embryos induced by low temperature, starvation, and osmotic pressure were counted and analyzed. [Result] In the low-temperature treatment group, the number of somatic embryos increased significantly at 4 ℃. While, there were no significant differences in the number of somatic embryos between treatment times at 10 ℃. Nevertheless, more somatic embryos were produced at 10 ℃ than at 28 ℃ (control temperature). Somatic embryogenesis was inhibited by the nutrient starvation treatment (i.e., phosphate, nitrogen, or inositol deficiency). In the osmotic stress group, the best results were observed following a 7-day culture in medium containing 40 g·L^-1 glucose. [Conclusion] 'Xinhai 16' somatic embryogenesis can be synchronized by incubating calli at 4 ℃ for 3 days, simulating phosphate or nitrogen starvation conditions for 5 days, or maintaining calli in a medium containing 40 g·L^-1 glucose for 7 days. The low-temperature treatment may provide optimal synchronization conditions.

Key words: Gossypium barbadense L; somatic embryogenesis; inducement; synchronization

中图分类号:

S562.01

郭家雁, 张霞, 丁喜莲, 李娟, 邓莉, 陈全家, 孙国清, 曲延英. 不同处理诱导新海16号体细胞胚胎同步化发生[J]. 棉花学报, 2017, 29(4): 385-392.

Guo Jiayan\, Zhang Xia, Ding Xilian, Li Juan, Deng Li, Chen Quanjia, Sun Guoqing, Qu Yanying. Synchronized Somatic Embryogenesis of 'Xinhai 16' Induced by Different Treatments[J]. Cotton Science, 2017, 29(4): 385-392.

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链接本文: https://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.gjyqyy.20170531

https://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2017/V29/I4/385

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