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棉花学报 ›› 2018, Vol. 30 ›› Issue (4): 326-337.doi: 10.11963/1002-7807.ghpsmz.20180720

• 研究与进展 • 上一篇    下一篇

棉花苗期耐低氮基因型初步筛选

贵会平,董强,张恒恒,王香茹,庞念厂,王准,刘记,郑苍松,付小琼,张西岭*,宋美珍*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳455000
  • 收稿日期:2017-12-13 出版日期:2018-07-15 发布日期:2018-07-15
  • 作者简介:贵会平(1988-),女,硕士, huiping.828@163.com。
  • 基金资助:
    国家重点研发计划——棉花化肥农药减施技术集成研究与示范(2017YFD0201900);中国农业科学院科技创新工程项目资助(植棉技术标准化创新团队);棉花生物学国家重点实验室基金(CB2017C06)

Preliminary Screening of Low Nitrogen-Tolerant Cotton Genotypes at Seedling Stage

Gui Huiping, Dong Qiang, Zhang Hengheng, Wang Xiangru, Pang Nianchang, Wang Zhun, Liu Ji, Zheng Cangsong, Fu Xiaoqiong, Zhang Xiling*, Song Meizhen*   

  1. Institute of Cotton Research of CAAS / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2017-12-13 Online:2018-07-15 Published:2018-07-15

摘要: 【目的】筛选耐低氮的棉花品种(系),挖掘棉花自身吸收利用氮素的潜力。【方法】以三大棉区不同年代有代表性的270个棉花品种为材料,利用沙培的试验方法,分析其在低氮和适氮两个水平下主要农艺性状的差异及相关性,通过主成分分析和隶属函数法筛选耐低氮品种。【结果】270个供试品种的7个农艺性状在不同供氮水平下变异系数较大,除SPAD值和低氮水平第一批的根干物质质量和总干物质质量外,变异系数都达到10以上,氮积累量变异系数达到60.02。第一批的SPAD、叶面积、根干物质质量相对值的最大值大于80%,第三批和第四批的SPAD、株高、根干物质质量、地上部干物质质量、总干物质质量相对值的最大值大于80%,说明所选材料中存在低氮水平下长势良好即耐低氮品种。【结论】初步筛选出中棉所35、中棉所69、豫棉12、新陆早12号、新陆早23号等32个耐低氮品种;筛选出中棉所64、中662、新陆中15号、新陆早53号等32个氮胁迫敏感型品种。

关键词: 棉花; 耐低氮; 氮素积累量; 主成分分析法; 隶属函数法

Abstract: [Objective] The aim of this study was to screen cotton varieties (lines) able to tolerate low nitrogen conditions, thereby tapping the potential of cotton to absorb and utilize nitrogen. [Method] This study analyzed the differences and correlations between the main cotton agronomic traits under two nitrogen levels using the sand culture method and 270 cotton varieties from different generations in three major cotton regions as materials. Principal component analysis and the membership function method were used to screen for low nitrogen-tolerant cotton varieties. [Result] The seven agronomic traits of 270 varieties varied greatly with different nitrogen levels. The coefficients of variation were all above 10 except for the SPAD value in four batches, and root biomass and cotton plant biomass in the first batch under low nitrogen level. The variation coefficient of nitrogen accumulation reached 60.02. The maximum relative value of SPAD, leaf area, and root biomass was greater than 80% in the first batch. The maximum relative values of SPAD, plant height, root biomass, shoot biomass, and cotton plant biomass were greater than 80% in the third and fourth batches, indicating that there are low nitrogen-tolerant genotypes in the selected materials. [Conclusion] Preliminary screening identified 32 low nitrogen-tolerant cultivars, including CCRI 35, CCRI 69, Yumian 12, Xinluzao 12, and Xinluzao 23. Furthermore, 32 varieties sensitive to nitrogen stress, including CCRI 64, CCRI 662, Xinluzhong 15, and Xinluzao 53, were identified.

Key words: cotton; low nitrogen tolerance; nitrogen accumulation; principal component analysis; membership function method

中图分类号: 
  • S562.01