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棉花学报 ›› 2015, Vol. 27 ›› Issue (2): 176-183.doi: 10.11963/issn.1002-7807.201502011

• 研究与进展 • 上一篇    下一篇

棉花S-腺苷蛋氨酸脱羧酶基因(GhSAMDC2/3/4)的克隆及其诱导表达分析

王凡龙,朱华国*,程文翰,刘永昌,成新琪,孙杰   

  1. 石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆 石河子 832003
  • 收稿日期:2014-07-28 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: zhgroger@sohu.com
  • 作者简介:王凡龙,男,硕士研究生,wangfanlong1988@163.com
  • 基金资助:

    国家自然科学基金(31301363);棉花生物学国家重点实验室开放课题(CB2014A12);兵团种质资源创新与功能基因发掘利用专项(2012BB049);国家“十二五”科技支撑计划(2011BAD35B05-3-6)

Cloning and Induced Expression Analysis of GhSAMDC2/3/4 in Cotton(Gossypium hirsutum L.)

Wang Fanlong, Zhu Huaguo*, Cheng Wenhan, Liu Yongchang, Cheng Xinqi, Sun Jie   

  1. College of Agronomy, Shihezi University/The Key Laboratory of Oasis Eco-agriculture of the Xinjiang Production and Construction Corps, Shihezi, Xinjiang 832003, China
  • Received:2014-07-28 Online:2015-03-15 Published:2015-03-15

摘要: 利用电子克隆结合RT-PCR技术克隆获得陆地棉(Gossypium hirsutum L.)S ̄腺苷蛋氨酸脱羧酶(S-adenosylmethionine decarboxylase, SAMDC)基因家族3个基因,分别命名为GhSAMDC2、GhSAMDC3和GhSAMDC4。序列分析显示,该基因cDNA包含的upstream ORF(uORF)和 main ORF(mORF)为植物SAMDC基因特征ORF,其中mORF长度分别为1068 bp、1110 bp和1032 bp,分别编码355、369和343个氨基酸。聚类分析表明,GhSAMDC2/3蛋白与可可树(Theobroma cacao)SAMDC聚为一类,且GhSAMDC2与GhSAMDC3蛋白亲缘关系最近;GhSAMDC4与拟南芥AtSAMDC4聚为一类。实时荧光定量PCR分析表明,GhSAMDC2在茎中表达相对较高,随着纤维发育其表达量不断增加,在纤维发育后期其表达量达到最高;GhSAMDC2/3/4在不同的胁迫条件下表现出不同的表达模式,GhSAMDC2受低温和干旱胁迫诱导最强烈,GhSAMDC3响应盐胁迫显著,GhSAMDC4受ABA诱导强烈。上述结果为进一步研究棉花SAMDC基因功能奠定了一定基础。

关键词: 棉花; 多胺; S-腺苷蛋氨酸脱羧酶基因; 非生物胁迫

Abstract: S-adenosine methionine decarboxylase(SAMDC) is one of three key enzymes in the polyamine synthesis pathway. GhSAMDC2/3/4(GhSAMDC2, GhSAMDC3 and GhSAMDC4) were acquired from Gossypium hirsutum L. by electronic cloning and reverse-transcription polymerase chain reaction(RT-PCR) technology. Sequence analysis showed that the gene cDNA contained two plant general SAMDC open reading frames(ORFs), the upstream ORF(uORF) and the main ORF(mORF). The mORFs of GhSAMDC2/3/4 were 1068, 1110 and 1032 bp, encoding 355, 369 and 343 amino acids, respectively. Cluster analysis showed that GhSAMDC2/3 and cacao tree SAMDC clustered within a class and GhSAMDC4 and AtSAMDC4 clustered within a separate category. Real-time PCR analysis showed GhSAMDC2/3/4 were expressed in all testing organization and fiber. GhSAMDC2 was obviously expressed in stem and late developed fiber. GhSAMDC2/3/4 were induced by abiotic stress, but GhSAMDC2 was strongly affected by low temperature and drought stress. GhSAMDC3 responded significantly to salt stress and GhSAMDC4 was strongly induced by ABA.

Key words: Cotton; Polyamines; S-adenosylmethionine decarboxylase gene; Abiotic stress

中图分类号: 
  • S562.035.3