欢迎访问《棉花学报》! 今天是

棉花学报 ›› 2018, Vol. 30 ›› Issue (6): 498-504.doi: 10.11963/1002-7807.zhxwgy.20181121

• 研究简报 • 上一篇    

不同遗传背景转基因抗虫棉Bt蛋白表达与氮代谢关系研究

赵红霞,王士杰,朱继杰,李 妙*,王国印*   

  1. 河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,石家庄 050035
  • 收稿日期:2018-03-27 出版日期:2018-11-15 发布日期:2018-11-15
  • 通讯作者: 李妙,limiao2003@sina.com;王国印,wgy1963@vip.sian.com
  • 作者简介:赵红霞(1977―),女,jifengzhhx@163.com
  • 基金资助:
    河北省科技支撑计划项目——多抗、优质、专用棉花种质资源创新及育种新技术研究(16226307D);河北省农林科学院粮油作物研究所青年基金项目——不同遗传背景棉花品种Bt基因表达与氮代谢关系研究(LYS2015005);国家科技重大专项子课题——转基因优质棉花新品种培育(2016ZX08005-003)

Relationship between Bt Protein Expression and Nitrogen Metabolism in Insect-Resistant Transgenic Cotton Lines with Different Genetic Backgrounds

Zhao Hongxia, Wang Shijie, Zhu Jijie, Li Miao*, Wang Guoyin*   

  1. Hebei Laboratory of Crop Genetics and Breeding/Hebei Academy of Agriculture and Forestry Sciences, Institute for Cereal and Oil Crops, Shijiazhuang 050035, China
  • Received:2018-03-27 Online:2018-11-15 Published:2018-11-15

摘要: 【目的】本研究旨在探明不同遗传背景抗虫棉Bt蛋白表达量与氮代谢关系,为筛选高抗虫棉花品种提供借鉴和参考。【方法】对41份材料在不同时期(4~6叶期、盛蕾期、结铃盛期)测定幼叶、幼蕾和幼铃中的Bt蛋白含量及各时期主茎功能叶中的氮代谢等指标,分析其相关性。【结果】不同遗传背景的转基因抗虫棉营养器官中Bt蛋白表达量与可溶性蛋白和游离氨基酸含量呈极显著正相关,与全氮相关性不显著;生殖器官中Bt蛋白表达量与可溶性蛋白含量、全氮含量呈极显著正相关,与游离氨基酸呈显著正相关。可溶性蛋白含量从苗期至蕾期、蕾期至铃期均匀下降时,Bt蛋白在中后期生殖器官中的表达量高。【结论】Bt蛋白表达量与可溶性蛋白含量高度正相关,后者可作为选育高抗虫品种的检测指标。

关键词: Bt蛋白表达; 氮代谢; 相关性; 抗虫棉; Bt蛋白表达; 氮代谢; 相关性

Abstract: [Objective] This study aimed to determine the relationship between Bt protein expression levels and nitrogen metabolism in insect-resistant transgenic cotton lines with different genetic backgrounds and to provide some recommendations for the selection of highly insect-resistant cotton cultivars. [Method] Bt protein contents and nitrogen metabolism-related indexes were measured in young leaves, buds, and bolls of 41 cotton lines at different developmental stages (4–6 leaf, flourishing bud, and flourishing boll setting stages), and the correlations among these indexes were analyzed. [Result] The expression level of Bt protein in vegetative organs was found to be highly significantly positively correlated with the contents of soluble protein and free amino acids but not that of total nitrogen. Bt protein expression levels in reproductive organs were highly significantly positively correlated with soluble protein and total nitrogen contents and significantly positively correlated with free amino acid content. While soluble protein levels showed a relatively steady decrease from seedling to bud stages and from bud to boll setting stages, the highest Bt protein contents were measured in reproductive organs in mid- to late periods. [Conclusion] The expression level of Bt protein had the highest positive correlation with soluble protein and can thus be used as a reliable index for selection of highly insect-resistant cotton cultivars.

Key words: insect-resistant cotton; Bt protein expression levels; nitrogen metabolism; correlation; insect-resistant cotton; Bt protein expression levels; nitrogen metabolism; correlation

中图分类号: 
  • S562:S311