基于两个陆地棉低世代群体定位纤维品质相关QTL

doi:10.11963/1002-7807.gxhysx.20201229

摘要/Abstract

摘要：

【目的】定位棉花纤维品质性状相关的数量性状位点（Quantitative trait locus,QTL）。【方法】以陆地棉高强纤维品系中棉所679和纤维品质一般的农垦5号为亲本构建包含200个单株的F₂群体及对应的F_2:3家系群体,对2个群体的纤维长度、断裂比强度等5个纤维品质性状进行检测。用6 688对简单重复序列（Simple sequence repeat, SSR）引物在双亲间筛选,得到149对多态性引物,以F₂为作图群体,使用QTL IciMapping软件进行连锁图谱构建,并对F₂及F_2:3群体进行QTL定位。【结果】根据F₂群体基因型信息构建了1张包含119个标记、28个连锁群、总长为1 173.5 cM（centiMorgan）的遗传连锁图谱。分别在F₂、F_2:3群体中检测到9个和11个与纤维品质性状相关的QTLs,这些QTLs分布在11个连锁群上。其中F₂群体的qFL-D11-1、qBT-D11-1与F_2:3群体的qFL-D11-1、qMIC-D11-1均定位在标记DPL0062与HAU0423之间,推测这些位点可能是控制纤维品质性状的重要QTL。【结论】利用多个群体进行QTL定位有益于发现稳定的QTL位点,控制纤维品质性状的基因可能成簇存在,为挖掘纤维品质性状相关基因及分子标记辅助育种奠定基础。

关键词: 陆地棉; 纤维品质; SSR; 连锁图谱; QTL

Abstract:

[Objective] The purpose of this study is to map quantitative trait loci (QTL) related to fiber quality traits in upland cotton. [Method] Using CCRI 679 with high-quality fiber and Nongken 5 as parents, we constructed a F₂ population with 200 individual plants and the corresponding F_2:3 population, and five fiber quality traits of the two populations were tested. We used 6 688 pairs of SSR (Simple sequence repeat) to screen between the two parents, and 149 polymorphic primers were obtained. The linkage map of F₂ genotype data and QTL mapping of F₂ and F_2:3 populations were carried out using the QTL IciMapping software. [Result] A genetic linkage map including 119 markers, 28 linkage groups and a total length of 1 173.5 cM (centiMorgan) was constructed. Nine and eleven QTLs related to fiber quality traits were detected in F₂ and F_2:3 population, respectively. These QTLs were distributed in 11 linkage groups. Among them, qFL-D11-1 and qBT-D11-1 of F₂ population and qFL-D11-1 and qMIC-D11-1 of F_2:3 population were all located between DPL0062 and HAU0423. It was speculated that these loci may be important QTLs controlling fiber quality traits. [Conclusion] Using multiple generations to map QTL is helpful to obtain stable QTL loci, and the genes controlling fiber quality traits may exist in clusters, and these QTLs will lay a foundation for mining fiber quality related genes and molecular marker-assisted breeding.

Key words: upland cotton; fiber quality; SSR; linkage map; QTL

郭晓豪,王寒涛,魏鑫,张晶晶,付小康,马亮,魏恒玲,喻树迅. 基于两个陆地棉低世代群体定位纤维品质相关QTL[J]. 棉花学报, 2021, 33(1): 33-42.

Guo Xiaohao,Wang Hantao,Wei Xin,Zhang Jingjing,Fu Xiaokang,Ma Liang,Wei Hengling,Yu Shuxun. QTL mapping of fiber quality traits in two lower generation populations of upland cotton[J]. Cotton Science, 2021, 33(1): 33-42.

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链接本文: http://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.gxhysx.20201229

http://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2021/V33/I1/33

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性状 Trait	亲本 Parents		群体 Population	均值 Mean	极大值 Maximum	极小值 Minimum	极差 Range	方差 Variance	偏度 Skewness	峰度 Kurtosis
性状 Trait	CCRI679	NK-5	群体 Population	均值 Mean	极大值 Maximum	极小值 Minimum	极差 Range	方差 Variance	偏度 Skewness	峰度 Kurtosis
纤维长度 FL /mm	33.2	23.2	F₂	28.34	33.40	23.60	9.80	3.28	0.17	-0.11
纤维长度 FL /mm	33.2	23.2	F_2:3	29.48	33.80	24.55	9.25	3.23	0.08	-0.49
长度整齐度指数 UI /%	85.3	81.0	F₂	84.78	88.00	81.20	6.80	1.64	-0.25	-0.31
长度整齐度指数 UI /%	85.3	81.0	F_2:3	85.07	87.60	81.20	6.40	1.23	-0.33	0.40
断裂比强度 BT /(cN·tex^-1)	35.7	23.7	F₂	30.57	38.80	22.10	16.70	7.73	-0.15	0.29
断裂比强度 BT /(cN·tex^-1)	35.7	23.7	F_2:3	32.73	39.30	27.25	12.05	3.92	0.04	0.23
马克隆值 MIC	5.4	5.0	F₂	4.96	6.30	3.00	3.30	0.34	-0.98	1.27
马克隆值 MIC	5.4	5.0	F_2:3	4.97	5.95	4.10	1.85	0.13	-0.09	-0.28
断裂伸长率 BE /%	7.0	6.2	F₂	6.71	7.00	6.20	0.80	0.02	-0.46	0.97
断裂伸长率 BE /%	7.0	6.2	F_2:3	5.72	7.30	4.90	2.40	0.18	0.69	0.76

性状 Trait	QTL	群体 Population	LOD	标记区间 Flanking marker	标记间距 Maker space /cM	加性效应 Additive effect	解释表型变异率 Phenotypic variation explained /%
纤维长度 FL /mm	qFL-A02-1	F₂	2.64	BNL3545-NAU3903	6.2	0.504 4	6.074 6
	qFL-D11-1	F₂	3.43	DPL0062-HAU0423	7.3	0.675 7	7.845 5
	qFL-D11-1	F_2:3	4.37	DPL0062-HAU0423	7.3	0.713 1	9.795 8
	qFL-L5-1	F_2:3	3.06	CGR5667-NAU3557	26.0	0.657 4	6.743 2
长度整齐度指数 UI /%	qUI-A05-1	F_2:3	4.84	NAU6611-BNL3977	16.5	0.168 9	9.013 3
	qUI-D04-1	F_2:3	3.93	Gh322-NAU5046	3.0	-0.021 2	6.904 5
	qUI-D09-1	F₂	2.78	CGR6692-NAU3915	2.9	0.462 0	6.394 4
	qUI-D11-1	F_2:3	2.82	Gh377-CGR5503	3.9	0.380 3	4.829 7
	qUI-L2-1	F_2:3	3.20	NAU5129-Gh325	48.7	-0.458 5	7.652 8
断裂比强度 BT /(cN·tex^-1)	qBT-A02-1	F₂	2.64	BNL3545-NAU3903	6.2	0.720 2	5.473 9
	qBT-D09-1	F₂	3.63	CGR6692-NAU3915	2.9	1.084 0	8.065 3
	qBT-D11-1	F₂	3.17	DPL0062-HAU0423	7.3	0.986 9	7.597 0
马克隆值 MIC	qMIC-D11-1	F₂	3.47	BNL3171-BNL3860	55.7	-0.407 4	4.818 3
	qMIC-D11-1	F_2:3	4.43	DPL0062-HAU0423	7.3	-0.139 8	10.311 7
	qMIC-L2-1	F_2:3	2.86	NAU2306-NAU5129	21.1	0.113 3	7.797 3
	qMIC-L3-1	F₂	2.82	DPL0135-DPL0319	40.9	0.303 2	3.814 6
断裂伸长率 BE /%	qBE-A02-1	F₂	2.67	BNL3545-NAU3903	6.2	0.040 3	7.133 5
	qBE-A09-1	F_2:3	3.62	Gh111-NAU4093	7.5	-0.154 9	7.420 2
	qBE-L1-1	F_2:3	3.89	NAU1298-BNL1227	9.1	-0.164 3	7.913 9
	qBE-L4-1	F_2:3	3.94	Gh638-NAU1042	28.9	-0.148 7	8.784 9