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棉花学报 ›› 2021, Vol. 33 ›› Issue (1): 33-42.doi: 10.11963/1002-7807.gxhysx.20201229

• 研究与进展 • 上一篇    下一篇

基于两个陆地棉低世代群体定位纤维品质相关QTL

郭晓豪1,2(),王寒涛2,魏鑫2,张晶晶2,付小康2,马亮2,魏恒玲2,喻树迅1,2,*   

  1. 1.河南科技学院,河南 新乡453000
    2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳455000
  • 收稿日期:2019-12-06 出版日期:2021-01-15 发布日期:2021-02-25
  • 通讯作者: 喻树迅 E-mail:864214693@qq.com
  • 作者简介:郭晓豪(1992―),男,硕士, 864214693@qq.com
  • 基金资助:
    国家现代农业产业技术体系棉花产业技术体系(CARS-15-06);中国农业科学院科技创新工程

QTL mapping of fiber quality traits in two lower generation populations of upland cotton

Guo Xiaohao1,2(),Wang Hantao2,Wei Xin2,Zhang Jingjing2,Fu Xiaokang2,Ma Liang2,Wei Hengling2,Yu Shuxun1,2,*   

  1. 1. Henan Institute of Science and Technology, Xinxiang, Henan 453000, China
    2. State Key Laboratory of Cotton Biology / Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China
  • Received:2019-12-06 Online:2021-01-15 Published:2021-02-25
  • Contact: Yu Shuxun E-mail:864214693@qq.com

摘要:

【目的】 定位棉花纤维品质性状相关的数量性状位点(Quantitative trait locus,QTL)。【方法】 以陆地棉高强纤维品系中棉所679和纤维品质一般的农垦5号为亲本构建包含200个单株的F2群体及对应的F2:3家系群体,对2个群体的纤维长度、断裂比强度等5个纤维品质性状进行检测。用6 688对简单重复序列(Simple sequence repeat, SSR)引物在双亲间筛选,得到149对多态性引物,以F2为作图群体,使用QTL IciMapping软件进行连锁图谱构建,并对F2及F2:3群体进行QTL定位。【结果】 根据F2群体基因型信息构建了1张包含119个标记、28个连锁群、总长为1 173.5 cM(centiMorgan)的遗传连锁图谱。分别在F2、F2:3群体中检测到9个和11个与纤维品质性状相关的QTLs,这些QTLs分布在11个连锁群上。其中F2群体的qFL-D11-1qBT-D11-1与F2:3群体的qFL-D11-1qMIC-D11-1均定位在标记DPL0062与HAU0423之间,推测这些位点可能是控制纤维品质性状的重要QTL。【结论】 利用多个群体进行QTL定位有益于发现稳定的QTL位点,控制纤维品质性状的基因可能成簇存在,为挖掘纤维品质性状相关基因及分子标记辅助育种奠定基础。

关键词: 陆地棉; 纤维品质; SSR; 连锁图谱; QTL

Abstract:

[Objective] The purpose of this study is to map quantitative trait loci (QTL) related to fiber quality traits in upland cotton. [Method] Using CCRI 679 with high-quality fiber and Nongken 5 as parents, we constructed a F2 population with 200 individual plants and the corresponding F2:3 population, and five fiber quality traits of the two populations were tested. We used 6 688 pairs of SSR (Simple sequence repeat) to screen between the two parents, and 149 polymorphic primers were obtained. The linkage map of F2 genotype data and QTL mapping of F2 and F2:3 populations were carried out using the QTL IciMapping software. [Result] A genetic linkage map including 119 markers, 28 linkage groups and a total length of 1 173.5 cM (centiMorgan) was constructed. Nine and eleven QTLs related to fiber quality traits were detected in F2 and F2:3 population, respectively. These QTLs were distributed in 11 linkage groups. Among them, qFL-D11-1 and qBT-D11-1 of F2 population and qFL-D11-1 and qMIC-D11-1 of F2:3 population were all located between DPL0062 and HAU0423. It was speculated that these loci may be important QTLs controlling fiber quality traits. [Conclusion] Using multiple generations to map QTL is helpful to obtain stable QTL loci, and the genes controlling fiber quality traits may exist in clusters, and these QTLs will lay a foundation for mining fiber quality related genes and molecular marker-assisted breeding.

Key words: upland cotton; fiber quality; SSR; linkage map; QTL