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棉花学报 ›› 2013, Vol. 25 ›› Issue (3): 205-210.

• 研究与进展 • 上一篇    下一篇

棉花转录因子GhWRKY4基因的克隆及特征分析

李光雷,范术丽,宋美珍,庞朝友,魏恒玲,喻树迅*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2012-05-04 出版日期:2013-05-15 发布日期:2013-05-15
  • 通讯作者: yu@cricaas.com.cn
  • 作者简介:李光雷(1986-),男,硕士,nxyzwswjslgl@126.com
  • 基金资助:

    国家高技术研究发展计划(2011AA10A102);国家棉花产业技术体系(CARS-18)

Molecular Cloning and Characterization Analysis of GhWRKY4, a Transcription Factor Gene in Cotton (Gossypium hirsutum L.)

LI Guang-lei, FAN Shu-li, SONG Mei-zhen, PANG Chao-you, YU Shu-xun*   

  1. Institute of Cotton Research, CAAS / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2012-05-04 Online:2013-05-15 Published:2013-05-15

摘要: 本研究从陆地棉中克隆得到GhWRKY4基因,全长cDNA是1281 bp,包含1083 bp的开放阅读框,编码的360个氨基酸属于IIcWRKY转录因子家族。分析GhWRKY4基因结构表明其有三个外显子和两个内含子。GhWRKY4蛋白的亚细胞定位实验表明其定位在洋葱表皮细胞核。实时荧光定量分析结果显示GhWRKY4在棉花的根、茎、叶和花中均有表达。染色体步移得到了GhWRKY4的5’端侧翼序列,生物信息学软件预测表明GhWRKY4包含一系列和胁迫基因调控相关的反式作用元件,表明GhWRKY4参与植物对盐害和冷害胁迫的反应。

关键词: GhWRKY4基因; 棉花; 转录因子; 胁迫

Abstract: In the present study, a WRKY gene, GhWRKY4, was cloned from cotton(Gossypium hirsutum L.). The full-length cDNA of GhWRKY4 was 1281 bp long and contained a 1083-bp open reading frame (ORF) encoding a putative group-IIc WRKY protein of 360 amino acids. Bioinformatic analysis of the GhWRKY4 gene revealed that GhWRKY4 consists of three exons and two introns. A subcellular localization assay confirmed that the protein product was targeted in onion epidermal cell nuclei. Quantitative RT-PCR indicated that GhWRKY4 is constitutively expressed in cotton roots, stems, leaves, and flowers. The 5' flanking region of GhWRKY4 was obtained by genome walking. Bioinformatic analysis revealed that GhWRKY4 contains a series of putative cis-acting elements involved in stress regulation. Further stress treatments combined with gene expression analysis indicated that GhWRKY4 is involved in regulation of salt and cold stress.

Key words: GhWRKY4 gene; Gossypium hirsutum L.; transcription factor; stress response

中图分类号: 
  • S562.035.3