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棉花学报 ›› 2011, Vol. 23 ›› Issue (4): 379-384.

• 专题与述评 • 上一篇    

农杆菌介导陆地棉转化和再生体系 的研究进展

解晓红,张换样,李 静,焦改丽,吴慎杰*   

  1. 山西省农科院棉花研究所,运城 044000
  • 收稿日期:2010-11-30 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: sj_wu@126.com
  • 作者简介:解晓红(1968-),女,学士, 副研究员,xxhwzq@126.com
  • 基金资助:

    国家自然基金-科学部主任基金(31040059);国家转基因专项(2008ZX08005-004(1-4));山西省自然基金(2008011059-2);山西省博士基金(YBSJJ0802);山西省科技攻关项目(20100311003-1)

Advances of Agrobacterium-mediated Transformation and Regeneration System of Upland Cotton

 XIE Xiao-hong, ZHANG Huan-yang, LI Jing, JIAO gai-li, WU Shen-jie*   

  1. Cotton Research Institute of Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000, China
  • Received:2010-11-30 Online:2011-07-15 Published:2011-07-15

摘要: 陆地棉通过体细胞胚胎发生的农杆菌介导转化体系的主要瓶颈在于:基因型限制、转化周期长、胚胎发生率低、易出现畸形胚等问题。针对这些问题,研究者对传统转化体系的各个环节进行了优化,并在拓展受体基因型、筛选高频再生品(种)系或株系、选择不同的组织或器官做受体和建立新的不依赖于组织培养和基因型限制的农杆菌转化体系等方面进行了探索和研究。

关键词: 棉花; 农杆菌介导转化; 转化体系; 再生体系

Abstract: Advance of Agrobacterium-mediated transformation and regeneration system of cotton(Gossypium hirsutum L.) is summarized. Genotype-dependent, long transformation period, low frequency of embryogenesis, and high rates of abnormal embryos are main bottlenecks of Agrobacterium-mediated transformation of cotton via somatic embryogenesis. To solve these problems, the researchers optimized traditional transformation systems, expanded recipient genotypes, screened high-frequency regeneration lines, selected different organs or tissues as recipient explants, and established new methods of Agrobacterium-mediated cotton transformation which is tissue culture- and genotypes-independent.

Key words:  cotton; Agrobacterium mediated transformation; transformation system; regeneration system

中图分类号: 
  • S562.035.3