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棉花学报 ›› 2010, Vol. 22 ›› Issue (6): 617-624.

• 专题与述评 • 上一篇    下一篇

RNA干扰及其对昆虫抗药性相关基因的沉默研究

唐 涛1, 2,刘雪源2,邱立红1*   

  1. 1.中国农业大学理学院应用化学系,北京 100193;2.湖南省植物保护研究所, 长沙 410125
  • 收稿日期:2010-06-21 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: lihongqiuyang@126.com
  • 作者简介:唐 涛(1980-),男,博士研究生,助理研究员,tanson_1@163.com
  • 基金资助:

    国家自然科学基金(30971943)

RNA Interference and Its Applications on Silencing of Insecticide-resistant Genes in Insects

TANG Tao1, 2, LIU Xue-yuan2, QIU Li-hong1*   

  1. 1. Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China; 2. Hunan Plant Protection Institute, Changsha 410125, China
  • Received:2010-06-21 Online:2010-11-15 Published:2010-11-15
  • Contact: lihongqiuyang@126.com

摘要: 以不同农药为主的防治策略对棉铃虫和小菜蛾等农业害虫的控制卓有成效,但易产生害虫抗药性问题。害虫抗药性与细胞色素P450酶系、酯酶、钙粘蛋白等酶或受体的生化与分子机理相关。RNA干扰(RNA interference,简称RNAi)作为分子生物学领域中功能基因及基因组研究的一种强有力工具,已逐渐用于昆虫抗药性相关基因的敲除研究并鉴定其功能。本文围绕RNAi的作用机理及其对昆虫抗药性相关基因的沉默研究展开综述,旨在为农业害虫及其抗药性治理提供新思路与新途径。

关键词: RNA干扰; 细胞色素P450酶; 酯酶; 钙粘蛋白; 抗药性

Abstract: There have been enormous economical losses in agriculture caused by devastating insect pests, such as cotton bollworm Helicoverpa armigera (Hübner) and diamondback moth Plutella xylostella (L.), etc. The pests could be effectively controlled by insecticides, however many kinds of the pests have showed serious resistance to different insecticides. Studies have proved that insect resistance related to the detoxification enzymes or receptors including cytochrome P450 enzymes, esterases and cadherin proteins, etc. Knockout of genes related with resistance in insect pests has been carried out by RNA interference (RNAi) recently, which is a powerful tool in molecular biology for researches on functional genes and genomes. This review focuses on the mechanisms of RNAi and its applications in silencing resistance-related genes in insects in vivo, aiming at providing novel ideas and approaches for insect controlling and resistant management in agricultural pests.

Key words: RNA interference; cytochrome P450s; esterase; cadherin protein; insecticide resistance

中图分类号: 
  • S435.622