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棉花学报 ›› 2010, Vol. 22 ›› Issue (6): 539-546.

• 研究与进展 • 上一篇    下一篇

棉酚降解菌株的分离、筛选及鉴定

杨 霞1,翁晓燕2,郭建林3,孙建义1*   

  1. 1. 浙江大学动物科学学院/动物分子营养学教育部重点实验室,杭州 310029;2. 浙江大学生命科学学院,杭州 310029;3. 浙江省淡水水产研究所,浙江 湖州 313001
  • 收稿日期:2010-03-03 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: jysun@zju.edu.cn
  • 作者简介:杨 霞 (1982-),女,博士,yxia1982@126.com
  • 基金资助:

    国家高技术研究发展计划 (2007AA100601)

Screening and Identification of Gossypol-degraded Strains Isolated from a Soil Microcosm

YANG Xia1, WENG Xiao-yan2, GUO Jian-lin3, SUN Jian-yi1*   

  1. 1. Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310029, China; 2. College of Life Science, Zhejiang University, Hangzhou 310029, China; 3. Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China
  • Received:2010-03-03 Online:2010-11-15 Published:2010-11-15
  • Contact: jysun@zju.edu.cn

摘要: 以醋酸棉酚为唯一碳源,从土壤中筛选到2株棉酚降解菌,命名为MM-2及RP-3。经形态学及分子生物学鉴定:MM-2与基因登录号为AF219122.1(Fusarium oxysporum)的同源性达93%,推测MM-2属于镰刀菌属;RP-3与EU294522.1(Rhodotorula mucilaginosa)的同源性达92%,故此推测RP-3属于红酵母属。棉酚降解菌在不同碳源及温度条件下生长试验表明:MM-2在葡萄糖中生长好于在棉酚中,葡萄糖中最适取种时间为48~72 h,棉酚中最适取种时间为60~84 h,最适生长温度均为30℃;RP-3在葡萄糖中的最适取种时间为24~48 h,最适生长温度在25~30℃之间。RP-3在棉酚中生长时,由于醋酸棉酚本身为黄色晶体,影响了OD值的结果,但仍能显示出对棉酚具有降解作用。

关键词: 棉酚; 生物降解; 菌种鉴定; 生长

Abstract: Two new strains named MM-2 and RP-3 were isolated from a soil microcosm and investigated due to the finding that they could degrade free-gossypol on agar plates. Through the morphological and molecular biological identification, MM-2 was confirmed homologous with Fusarium oxysporum as high as 93%, presumably that it might belong to Fusarium. RP-3 was confirmed homologous with Rhodotorula mucilaginosa as high as 92%, presumably that it might belong to Rhodotorula. The growth characters of gossypol-degraded strains in different carbon sources,at different temperatures were compared. The results showed that the optimal incubation conditions of MM-2 were 48~72 h at 30℃ in glucose medium and 60~84 h at 30℃ in gossypol medium. The optimum growth conditions of RP-3 were 24~48 h at 25~30℃ in glucose medium. The OD value of RP-3 in gossypol medium could not be studied exactly because of the presence of gossypol which is a yellow pigment, however, the degradation of gossypol by strain RP-3 was in evidence.

Key words:  gossypol; biodegradation; identification; growth

中图分类号: 
  • S562