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棉花学报 ›› 2017, Vol. 29 ›› Issue (1): 59-69.doi: 10.11963/issn.1002-7807.201701007

• 研究与进展 • 上一篇    下一篇

陆地棉Pht1家族成员的全基因组鉴定及表达分析

晁毛妮1,*(),张志勇1,*(),宋海娜2,李成奇1,张新1,胡根海1,张金宝1,王清连1,**()   

  1. 1. 河南科技学院生命科技学院/现代生物育种河南省协同创新中心,河南 新乡 453003
    2. 平顶山学院,河南 平顶山 467000
  • 收稿日期:2016-01-08 出版日期:2017-01-15 发布日期:2017-01-15
  • 通讯作者: 晁毛妮,张志勇,王清连 E-mail:chaomaoni@126.com;z_zy123@163.com;wangql985@163.com
  • 作者简介:晁毛妮(1987―),女,博士,讲师,chaomaoni@126.com;张志勇,z_zy123@163.com;*,同等贡献。**
  • 基金资助:
    国家自然科学基金(31601347);河南省高等学校重点科研项目计划(15A210001);河南科技学院高层次人才计划(2014004)

Genome-Wide Identification and Expression Analysis of Pht1 Family Genes in Cotton (Gossypium hirsutum L.)

Chao Maoni1,*(), Zhang Zhiyong1,*(), Song Haina2, Li Chengqi1, Zhang Xin1, Hu Genhai1, Zhang Jinbao1, Wang Qinglian1,**()   

  1. 1. College of Life Science and Technology, Henan Institute of Science and Technology / Henan Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, Henan 453003, China
    2. Pingdingshan University, Pingdingshan, Henan 467000, China
  • Received:2016-01-08 Online:2017-01-15 Published:2017-01-15
  • Contact: Maoni Chao,Zhiyong Zhang,Qinglian Wang E-mail:chaomaoni@126.com;z_zy123@163.com;wangql985@163.com

摘要: 目的 磷是植物三大必需矿质元素之一,对植物的生长发育至关重要。Pht1家族的磷酸盐转运蛋白在植物磷吸收和转运方面具有重要作用,然而关于该基因的系统研究工作尚很少开展。本研究旨在进行Pht1家族成员全基因组鉴定和表达模式分析。方法 通过生物信息学的方法对陆地棉Pht1家族成员的基因结构、编码蛋白质的结构、染色体定位、基因复制和表达情况等进行全面分析。结果 (1)在陆地棉的基因组中共鉴定到17个磷酸盐转运蛋白基因(GhPT),其中A亚组包含8个GhPT,D亚组包含9个GhPT;(2)棉花GhPT蛋白之间序列相似性很高,并均具有12个疏水的跨膜区域;(3)进化分析表明这些GhPT蛋白主要聚为2大组(GroupⅠ和GroupⅡ),位于同一组的GhPT的编码基因大部分具有相似的内含子/外显子分布模式;(4)17个GhPT基因不均匀分布在A亚组和D亚组中的5条染色体上,串联复制和片段复制可能导致GhPT基因在陆地棉中的扩增;(5)表达模式分析表明,GhPT6和GhPT14在根中表达量最高,且同时响应低磷和低钾胁迫诱导并上调表达。结论 这些结果将有助于深入了解Pht1家族基因的功能及离子信号途径相互作用的分子机制。关键词:陆地棉;基因家族;GhPT基因;磷酸盐转运蛋白;进化分析;表达模式

关键词: upland cotton; gene family; GhPT; Pi transporter; phylogenetic analysis; expression pattern

Abstract:

[Objective] Phosphorus is one of the three most essential macronutrients required by plants, and is important for plant growth and development. Phosphate (Pi) transporter proteins in the Pht1 family play important roles in Pi uptake and translocation in plants. However, few systematic studies have been performed on this topic. In this study, we aimed to identify the Pht1 family members in the upland cotton genome and analyze their expression patterns. [Method] The gene structure, chromosomal localization, gene duplication, and expression of Pht1 family members in upland cotton, as well as the structure of the encoded protein, were analyzed using bioinformatics methods. [Results] (1) A total of 17 GhPT genes were identified in the upland cotton genome, with eight and nine GhPT genes distributed on the A and D subgenomes, respectively. (2) The GhPT protein sequences were highly similar, and included 12 transmembrane regions. (3) The GhPT proteins were divided into Groups I and II based on phylogenetic analyses. Most GhPT genes within the same group had similar exon and intron organizations. (4) The chromosomal distribution pattern revealed that GhPT genes were unevenly distributed on five chromosomes of the A and D subgenomes. Segmental and tandem duplications were the major contributors to the increase in the number of GhPT genes in upland cotton. (5) The GhPT expression patterns revealed that GhPT6 and GhPT14 were highly expressed in roots. Moreover, the expression levels of these genes were up-regulated under low phosphorus and low potassium conditions. [Conclusion] These results may help to elucidate the functions of GhPT genes and the molecular mechanisms underlying the cross-talk among ion signals in plants.

Key words: upland cotton; gene family; GhPT; Pi transporter; phylogenetic analysis; expression pattern