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棉花学报 ›› 2016, Vol. 28 ›› Issue (4): 399-406.doi: 10.11963/issn.1002-7807.201604012

• 专题与述评 • 上一篇    下一篇

棉花单核苷酸多态性标记研究进展

王振玉,李威**,周晓箭,裴小雨,刘艳改,周克海,张文生,孟清芹,王海风,葛勇,李莹,刘俊芳,马雄风*,杨代刚*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2015-10-19 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: maxf_caas@163.com,yangdg2013@qq.com
  • 作者简介:王振玉(1989―),男,硕士,wangzhenyu89@126.com;**并列第一作者
  • 基金资助:
    国家自然科学基金(31401431);中央级公益性科研院所基本科研业务费增量项目(0032015012)

Review of Single Nucleotide Polymorphism Markers in Cotton

Wang Zhenyu, Li Wei**, Zhou Xiaojian, Pei Xiaoyu, Liu Yangai, Zhou Kehai, Zhang Wensheng, Meng Qingqin, Wang Haifeng, Ge Yong, Li Ying, Liu Junfang, Ma Xiongfeng*, Yang Daigang*   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2015-10-19 Online:2016-07-15 Published:2016-07-15

摘要: 单核苷酸多态性标记已在农作物研究中得到广泛应用并取得重大进展。为了便利棉花SNP(Single nucleotide polymorphism)标记的研究和应用,介绍了利用基因芯片、简化基因组测序、重测序等在棉花中开发SNP标记的方法,综述了SNP标记在棉花遗传图谱构建、数量位点的定位和分子标记辅助育种、基因组测序以及系统进化等研究中的应用。并对异源四倍体棉花中SNP标记开发时,同源序列位点和部分同源序列位点上的SNP标记辨别问题进行了系统探讨,对其快捷的开发、检测方式和在数量基因定位中的应用前景进行了展望。

关键词: 棉花; 分子标记; 单核苷酸多态性; 表达序列标签; 基因组测序; 遗传图谱; 进展

Abstract: Single nucleotide polymorphism (SNP) markers have been widely used in crop studies and significant progress has been made in recent years. In this article, we examine the development and research progress of SNP markers in cotton, including the methods of using gene chips, simplifying genome sequencing and resequencing to develop the SNP markers in cotton, and the applications of SNP markers in cotton such as genetic mapping, quantitative trait loci analysis, molecular marker-assisted breeding, genome sequencing, and phylogenetic analysis. The present problems in the development and application of SNP markers on homologous and homoeologous loci in allotetraploid cotton are also discussed. The anticipated prospects for SNP markers are also discussed in terms of rapid detection and quantitative trait loci mapping, which is expected to help the development and application of SNP markers in cotton in future.

Key words: cotton; molecular markers; single nucleotide polymorphism markers; expressed sequence tag; genome sequencing; genetic map; progress

中图分类号: 
  • S562.035