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棉花学报 ›› 2016, Vol. 28 ›› Issue (3): 234-241.doi: 10.11963/issn.1002-7807.201603006

• 研究与进展 • 上一篇    下一篇

通过转CaM基因提高了棉花抗寒性

赵存鹏,郭宝生,王凯辉,刘素恩,王兆晓,刘冬艳,耿军义*   

  1. 河北省农林科学院棉花研究所/农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室/国家棉花改良中心河北分中心,河北 石家庄 050051
  • 收稿日期:2015-09-21 出版日期:2016-05-15 发布日期:2016-05-15
  • 通讯作者: gengjunyi66@126.com
  • 作者简介:赵存鹏(1985―),男,硕士研究生,助理研究员,zhaocunpeng@126.com
  • 基金资助:
    棉花生物学国家重点实验室开放课题基金(CB2014A14);国家科技重大专项——转基因生物新品种培育(2014ZX08005-005)

Improving Cold Resistance by Transferring the CaM Gene into Cotton Plants

Zhao Cunpeng, Guo Baosheng, Wang Kaihui, Liu Su’en, Wang Zhaoxiao, Liu Dongyan, Geng Junyi*   

  1. Cotton Research Institute, Hebei Academy of Agriculture and Forestry Sciences / Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture / National Cotton Improvement Center Hebei Branch, Shijiazhuang, Hebei 050051, China
  • Received:2015-09-21 Online:2016-05-15 Published:2016-05-15

摘要: 为了获得耐低温棉花新材料,通过花粉管通道法获得了转CaM基因棉花,经过分子检测得到了3个转基因株系。同时对得到的转基因株系的T4代(C1、C2、C3)和棉花受体(CK)在低温处理后的相关生理生化指标进行了初步测定。发现在低温(4 ℃)处理24 h后,C1、C2、C3叶片中的丙二醛(MDA)含量明显低于对照,超氧化物歧化酶(SOD)、过氧化物酶(POD)的活力、可溶性糖的含量明显高于对照,而常温下(23 ℃)测定值没有明显的差异。表明转CaM基因棉花在低温时能够通过减轻叶片膜脂过氧化程度,提高抗氧化酶活力等反应来缓解低温对棉花叶片的损伤。本研究筛选出了3个具有一定抗寒能力的转基因株系,为下一步选育抗寒新品种奠定了基础,也为其他棉花抗寒转基因技术提供了理论依据。

关键词: 棉花; CaM基因; 低温胁迫; 生理特性; 膜脂过氧化; 抗氧化酶; 抗寒性

Abstract: This study aimed to obtain cotton (Gossypium hirsutum) materials resistant to low temperatures. The pollen tube pathway was employed to obtain transgenic CaM cotton materials, and three transgenic lines were profiled by molecular detection. Transgenic T4 lines (C1, C2, C3) and a control (CK) were used as materials for analysis of physiological and biochemical indexes before and after low temperature stress. After 24 h of low temperature (4 ℃) treatment, C1, C2, and C3 leaf malondialdehyde content was significantly lower than that in the control, while the activity of superoxide dismutase and peroxidase, and the soluble sugar content were significantly higher than that in the control; however, no significant differences were observed at room temperature (23 ℃). Comprehensive analysis revealed that under low temperature stress, transgenic CaM cotton can ease low temperature damage through reducing membrane lipid peroxidation in the leaf, and by increasing antioxidant enzyme activity. In this study, three transgenic lines were selected with cold resistance ability to lay a foundation for the breeding of new cotton materials with low temperature tolerances, and to provide a theoretical basis for the use of cotton transgenic technology for resisting low temperatures.

Key words: cotton; CaM gene; low temperature stress; physiological characteristic; membrane lipid peroxidation; antioxidant enzyme; cold resistance

中图分类号: 
  • S562.03