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棉花学报 ›› 2016, Vol. 28 ›› Issue (3): 208-214.doi: 10.11963/issn.1002-7807.201603003

• 研究与进展 • 上一篇    下一篇

植酸酶基因PhyA对陆地棉产量性状的影响

柯会锋,王省芬,吴立强,李志坤,张艳,张桂寅,马峙英*   

  1. 河北农业大学/教育部华北作物种质资源研究与利用重点实验室,河北 保定 071001
  • 收稿日期:2015-10-25 出版日期:2016-05-15 发布日期:2016-05-15
  • 通讯作者: mzhy@hebau.edu.cn
  • 作者简介:柯会锋(1973―),男,硕士,kehuifeng123@126.com
  • 基金资助:
    国家科技重大专项——转基因生物新品种培育(2009ZX08005-021B);河北省科技支撑计划(14226308D);河北省棉花产业技术体系

Influences of Transferring the PhyA Gene to Cotton (Gossypium hirsutum) on Yield Characteristics

Ke Huifeng, Wang Xingfen, Wu Liqiang, Li Zhikun, Zhang Yan, Zhang Guiyin, Ma Zhiying*   

  1. Agriculture University of Hebei / North China Key Laboratory of Crop Germplasm Resources, Education Ministry of China, Baoding, Hebei 071001, China
  • Received:2015-10-25 Online:2016-05-15 Published:2016-05-15

摘要: 在人工控制(培养基、水培、沙培)条件下,植酸酶基因PhyA具有分解利用培养基质植酸磷、提高磷素利用效率的功能,但在田间的表现目前尚未明确。在前期完成的PhyA转基因陆地棉新材料盆栽试验基础上,将其种植在田间条件下,研究PhyA基因对棉花产量性状的影响。结果表明,部分转基因棉花新材料的单株结铃数、铃重、衣分、籽棉产量、皮棉产量与野生型对照存在显著差异,PhyA在田间条件下具有改良转基因棉花部分产量性状的能力。在此基础上,遴选出2个转PhyA棉花优良新品系G3、G2,可用作今后转植酸酶基因棉花新品种培育的基础材料。

关键词: 植酸酶基因PhyA; 田间条件; 陆地棉; 产量性状; 植酸磷

Abstract: Phosphorus (P) is an important nutrient for plant growth, and only the inorganic form (Pi) dissolved in soil is readily available for uptake. However, abundant P exists in the soil as organic P (Po), of which more than 50% is phytate. Phytase is a specialized phosphatase that catalyzes the sequential hydrolysis of phytate to produce less phosphorylated myo-inositol derivatives and inorganic phosphate. In recent years, numerous phytase genes have been demonstrated to be important for utilizing Pi from phytate in transgenic plants under controlled conditions, such as in solid nutrition medium, hydroponic medium, sterilized vermiculite, and sand culture. However, the capacity of transgenic plants to access phosphate when grown in the field has remained unclear. Our previous study obtained some transgenic cotton lines containing the phytase gene PhyA, and their ability to utilize organic phosphate under hydroponic and vermiculite culture was demonstrated. Using these PhyA-overexpression lines under field conditions, 10 yield-related traits were investigated and analyzed to determine the influence of the PhyA gene on yield characteristics under field conditions. This revealed the numbers of bolls per plant, boll weight, lint percentage, seed cotton yield, and the lint yield of partially transgenic cotton lines were significantly higher than observed in the wild-type control. Based on these yield traits, two elite transgenic cotton lines, G3 and G2, were selected for their higher yield characteristics. These lines have great potential for use in future transgenic cotton breeding programs.

Key words: phytase gene; field conditions; upland cotton; yield characteristics; phytate

中图分类号: 
  • S562.03