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棉花学报 ›› 2016, Vol. 28 ›› Issue (2): 122-128.doi: 10.11963/issn.1002-7807.201602004

• 研究与进展 • 上一篇    下一篇

棉花耐盐相关基因GhVP的表达及功能分析

赵小洁1,2,穆敏1,2*,陆许可1,阴祖军1,王德龙1,王俊娟1,樊伟丽1,王帅1,叶武威1**   

  1. 1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业部棉花遗传改良重点开放实验室,河南 安阳 455000;2. 河南大学,河南 开封 475000
  • 收稿日期:2015-03-11 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: yew158@163.com
  • 作者简介:赵小洁(1990―),女,硕士,15036566999@163.com;*共同第一作者,穆敏(1990―),女,硕士,15890348192@163.com
  • 基金资助:
    国家科技重大专项——转基因生物新品种培育(2014ZX0800504B)

Expression and Functional Analysis of a Salt Tolerance-Related Gene from Cotton

Zhao Xiaojie1,2, Mu Min1,2, Lu Xuke1, Yin Zujun1, Wang Delong1, Wang Junjuan1, Fan Weili1, Wang Shuai1, Ye Wuwei1*   

  1. 1. State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan 455000, China; 2. Henan University, Kaifeng, Henan 475000, China
  • Received:2015-03-11 Online:2016-03-15 Published:2016-03-15

摘要: 为了研究GhVP基因在棉花中的表达特性和功能,克隆了棉花GhVP基因,构建pBI121-GhVP∷GFP荧光表达载体,采用基因枪技术转化洋葱内表皮细胞,结果显示GhVP基因编码蛋白定位于细胞质膜上。将构建的pBI121-GhVP∷GFP荧光表达载体转化棉花花粉,花粉荧光明显增强,说明该基因可以在棉花中表达,为GhVP基因可以在棉花中表达提供了依据,为培育棉花耐盐新材料奠定了基础。

关键词: 棉花; GhVP; 亚细胞定位; 花粉; 瞬时表达

Abstract: To develop an expression system to study the function of the GhVP gene in cotton (Gossypium hirsutum), we successfully cloned GhVP and used it to construct a pBI121-GhVP∷GFP fluorescence expression vector. We transformed the vector into onion (Allium cepa) epidermal cells using gene gun technology and found that the GhVP protein was located on the cytomembrane. The pBI121-GhVP∷GFP vector was then transformed into cotton pollen. Detection of green fluorescence in the pollen indicated that the gene was successfully expressed from the pBI121-GhVP∷GFP vector. These results lay the foundation for the study of GhVP in cotton and the development of salt tolerance breeding.

Key words: cotton; GhVP; subcellular localization; pollen; transient expression

中图分类号: 
  • S562.035