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棉花学报 ›› 2016, Vol. 28 ›› Issue (2): 95-103.doi: 10.11963/issn.1002-7807.201602001

• 研究与进展 •    下一篇

半野生棉与亚洲棉对草甘膦抗性评价及比较分析

王园园1,2,刘方1,周忠丽1,蔡小彦1,王玉红1,黎绍惠1,王春英1,张香娣1,王星星1,王坤波1*   

  1. 1.棉花生物学国家重点实验室/中国农业科学院棉花研究所,河南 安阳 455000;2. 河南科技学院,河南 新乡 453003
  • 收稿日期:2015-04-19 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: wkbcri@cricaas.com.cn;wkbcri@163.com
  • 作者简介:王园园(1986―),女,博士研究生,wangyy1007@163.com
  • 基金资助:
    国家科技重大专项——转基因生物新品种培育(2014ZX08005-003);国家科技支撑计划(2013BAD01B03);农业部保种项目(2014NWB034)

Evaluation and Comparative Analysis of Natural Resistance to Glyphosate in Gossypium hirsutum races and Gossypium arboreum L.

Wang Yuanyuan1,2, Liu Fang1, Zhou Zhongli1, Cai Xiaoyan1, Wang Yuhong1, Li Shaohui1, Wang Chunying1, Zhang Xiangdi1, Wang Xingxing1, Wang Kunbo1*   

  1. 1. State Key Laboratory of Cotton Biology / Institute of Cotton Research of CAAS, Anyang, Henan 455000, China; 2. Henan Institute of Science and Technology, Xinxiang, Henan 453003, China
  • Received:2015-04-19 Online:2016-03-15 Published:2016-03-15

摘要: 采用叶片棉球定位鉴定法,初步鉴定并评价了半野生棉和亚洲棉对草甘膦的自然抗性,结果表明:参试材料抗性水平从高抗至高感分6级,极端抗性水平相差33.3倍(0.2%对0.006%)。其中,半野生棉对草甘膦的自然抗性分级丰富,抗性水平分布自高抗至高感;但大部分半野生棉不耐草甘膦,近86%(346份)的材料抗性水平处于低耐级别以下。而亚洲棉抗性水平都处于高抗至低耐的4个级别,抗性多样性较差,但整体抗性水平较高,70.3%(71份)表现为耐或以上级别。从半野生棉和亚洲棉中分别筛选到2份和5份高抗材料,半野生棉中有1份高感材料,亚洲棉中无对草甘膦敏感材料。本研究报道的极端材料为棉花草甘膦抗性遗传机制研究、新品种的培育、内源抗性基因的挖掘和感抗机理研究,奠定了良好的材料基础。

关键词: 半野生棉; 亚洲棉; 草甘膦; 抗性评价

Abstract: While glyphosate makes weed management easy and efficient by controlling almost all emerged weeds, it severely influences the normal growth of cotton. The objective of this research was to evaluate natural glyphosate resistance by using tampons with different concentrations of glyphosate placed at specific positions on leaves of different plants and then rating the plants according to their reaction to glyphosate. The plants were graded into six levels of glyphosate resistance, with the range of the concentrations used (0.200% vs 0.006%) being 33.3 times higher than those that could be used in practice. In particular, Gossypium hirsutum races, whose resistance was distributed across all six levels, had more variability in glyphosate resistance than Gossypium arboreum. However, nearly 86% of G. hirsutum races had low or no tolerance to glyphosate while over 70% of G. arboreum plants were tolerant, revealing that G. arboreum is more glyphosate tolerant overall. This was also supported by evidence that only two accessions of G. hirsutum exhibited high resistance to glyphosate while five germplasms of G. arboreum were highly resistant. Furthermore, one semi-wild accession of G. hirsutum was susceptible to glyphosate, but no susceptible plants were found for G. arboreum. These materials provide a base for further in-depth study of the genetic mechanism of glyphosate-resistance, glyphosate-resistant cotton breeding, and internal resistant gene mining.

Key words: Gossypium hirsutum races; Gossypium arboreum; glyphosate; resistant evaluation

中图分类号: 
  • S562.034