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棉花学报 ›› 2016, Vol. 28 ›› Issue (1): 52-64.doi: 10.11963/issn.1002-7807.201601007

• 研究与进展 • 上一篇    下一篇

亚洲棉(Gossypium arboreum L.)纤维次生壁加厚期NAC基因的鉴定与表达分析

王中娜1,2,商海红2,陈婷婷2,巩万奎2,刘爱英2,李俊文2,石玉真2,龚举武2,葛群2,王琳2,李伟2,李鹏涛2,白志川1,于杰1*,袁有禄2*   

  1. 1.西南大学园艺园林学院,重庆 400716;2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,农业部棉花生物学与遗传育种重点实验室,河南 安阳 455000
  • 收稿日期:2015-02-11 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: yylcri@126.com
  • 作者简介:王中娜(1988―),女,硕士研究生,gzq668110@126.com
  • 基金资助:
    国家自然科学基金(31000732);国家高技术研究发展计划(863计划)(2012AA101108);中国农业科学院科技创新工程;河南省基础与前沿技术研究计划(142300413202)

Molecular Cloning and Expression Analysis of NAC Genes Specifically Expressed in the Fibers of Gossypium arboreum L.

Wang Zhongna1,2, Shang Haihong2, Chen Tingting2, Gong Wankui2, Liu Aiying2, Li Junwen2, Shi Yuzhen2, Gong Juwu2, Ge Qun2, Wang Lin2, Li Wei2, Li Pengtao2, Bai Zhichuan1, Yu Jie1*, Yuan Youlu2*   

  1. 1. Horticulture and Landscape College, Southwest University, Chongqing 400716, China; 2. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministry of Agriculture, Anyang 455000, China
  • Received:2015-02-11 Online:2016-01-15 Published:2016-01-15

摘要: 基于亚洲棉29729条纤维相关EST(Expressed sequence tag)序列和亚洲棉基因组序列,并结合雷蒙德氏棉(G. raimondii)NAC(NAM/ ATAF /CUC)转录因子家族的研究结果,利用生物信息学方法,鉴定出143个亚洲棉NAC转录因子家族基因;利用实时荧光定量PCR 技术对30个NAC转录因子构建表达图谱,鉴定出7个基因在亚洲棉纤维细胞次生壁加厚期优势表达,进一步明确了它们在亚洲棉、雷蒙德氏棉和陆地棉中的表达模式差异。利用RT-PCR(Reverse transcription-polymerase chain reaction)技术克隆得到了3个NAC基因全长cDNA,将其命名为GaNAC20、GaNAC09和GaNAC69,初步的生物信息学分析发现GaNAC20和GaNAC09基因均含有3 个外显子和2 个内含子,GaNAC69 含有4个外显子和3个内含子,为进一步研究奠定了良好基础。

关键词: 亚洲棉(G. arboreum L.); 棉纤维; NAC转录因子; 序列分析; 结构分析; 表达模式

Abstract: A total of 29,729 fiber-related expressed sequence tags and the genome sequence of Gossypium arboreum L. were combined with NAC transcription factor family research findings from G. raimondii using bioinformatics to identify 143 NAC transcription factor genes from G. arboreum L. Quantitative real-time PCR was used to analyze the expression patterns of 30 of these genes, and the expressions of seven fiber advantage expressed genes were analyzed in G. hirsutum L., G. arboreum L., and G. raimondii. These seven genes showed different expression patterns in cotton plants with different genomes, suggesting that significant changes have taken place in these genes during the evolution of the cotton genome. The full-length cDNAs of three of these genes(designated GaNAC20, GaNAC09, and GaNAC69) were cloned successfully. Bioinformatic analysis showed that GaNAC20 and GaNAC09 are composed of three exons and two introns, while GaNAC69 has four exons and three introns. The encoded proteins have an NAM domain structure, which is typical of the NAC family of proteins. Multiple sequence alignment and phylogenetic tree analysis predicted the function of these proteins, and laid the foundation for the study of NAC transcription factors in the evolution of the cotton genome.

Key words: G. arboreum L.; cotton fiber; NAC transcription factors; sequence analysis; structural analysis; expression patterns

中图分类号: 
  • S562.035.3