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棉花学报 ›› 2015, Vol. 27 ›› Issue (6): 576-582.doi: 10.11963/issn.1002-7807.201506010

• 研究简报 • 上一篇    下一篇

莳萝子挥发油抗棉花黄萎病菌活性及其作用机理

杨生强   

  1. Yang Shenqiang
  • 收稿日期:2015-01-22 出版日期:2015-11-15 发布日期:2015-11-15
  • 作者简介:杨生强(1980―),男,1243884393@qq.com
  • 基金资助:
    塔里木盆地生物资源保护利用兵团重点实验室开放课题(BRZD1205)

In vitro Antifungal Activity and Antifungal Mechanism of Essential Oil from Dill (Anethum graveolens L.) on Verticillium dahliae Kleb.

Yang Shenqiang   

  1. 29 Company, 12 Regiment, Division No.1, Xinjiang Production and Construction Crops, Alar, Xinjiang 843300, China
  • Received:2015-01-22 Online:2015-11-15 Published:2015-11-15

摘要: 研究莳萝子挥发油抗棉花黄萎病菌活性和作用机理,为开发植物源防治棉花黄萎病菌农药提供理论依据。采用微量稀释法测定莳萝子挥发油对棉花黄萎病菌的最低抑菌浓度(MIC),用带毒平板法测定莳萝子挥发油对棉花黄萎病菌菌丝抑制作用。研究莳萝子挥发油对棉花黄萎病菌孢子萌发和生物量的影响。采用碘化吡啶染色法测定莳萝子挥发油对棉花黄萎病菌孢子细胞膜的损坏。结果表明,莳萝子挥发油对棉花黄萎病菌最低抑菌浓度(MIC)为0.625 μL·mL-1,挥发油对棉花黄萎病菌生物量、孢子萌发和菌丝抑制作用呈浓度依赖关系。由碘化吡啶染色试验结果可知莳萝子挥发油能严重破坏棉花黄萎病菌孢子的细胞膜而达到抑菌作用。

关键词: 莳萝子挥发油; 棉花黄萎病; 抑菌活性; 最低抑菌浓度

Abstract: The objectives of the present study were to assess the antifungal effect of dill seed essential oil (EO) against V. dahliae and describe its mechanism of action. The microbroth dilution method was used at the minimal inhibitory concentration (MIC) and the dry mycelium weight and spore germination of the tested V. dahliae were also determined in a liquid culture to evaluate the anti-V. dahliae activity in vitro. The results showed EO was active against V. dahliae and MIC was 0.625 μL·mL-1. Mycelial growth, mycelium weight and spore germination were inhibited by the EO in a dose-dependent manner. The results show that upon treatment of cells with EO, propidium iodide penetrated V. dahliae through a lesion in its plasma membrane. Thus, our results demonstrated that EO could be a potential source of plant-derived fungicides to control V. dahliae.

Key words: Anethum graveolens L.; Verticillium dahlia; antifungal activity; minimal inhibitory concentration

中图分类号: 
  • S435.621