欢迎访问《棉花学报》! 今天是

棉花学报 ›› 2015, Vol. 27 ›› Issue (5): 489-493.doi: 10.11963/issn.1002-7807.201505014

• 研究简报 • 上一篇    下一篇

EPSPS外源基因对陆地棉农艺性状和纤维品质的影响

燕树锋1,2,刘海芳3,铁双贵2,祝水金1*   

  1. 1. 浙江大学农业与生物技术学院农学系,浙江 杭州310085;2. 河南省农业科学院粮食作物研究所,河南 郑州450002;3. 济源市农业局,河南 济源454650
  • 收稿日期:2015-01-26 出版日期:2015-09-15 发布日期:2015-09-15
  • 通讯作者: shjzhu@zju.edu.cn
  • 作者简介:燕树锋(1982-),男,博士,助理研究员,sf_yan1983@126.com
  • 基金资助:
    国家科技重大专项“转基因生物新品种培育”(2015ZX08005-005)

The Effects of an Exogenous EPSPS Gene on the Agronomic Traits and Fiber Quality of Upland Cotton

Yan Shufeng1,2, Liu Haifang3, Tie Shuanggui2, Zhu Shuijin1*   

  1. 1. Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310085, China; 2. Cereal Crops Research InstituteHenan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3. Jiyuan Agriculture Bureau, Jiyuan, Henan 454650, China
  • Received:2015-01-26 Online:2015-09-15 Published:2015-09-15

摘要: 以G6-1至G6-19等19个转基因抗草甘膦除草剂棉花种质为材料,以其非转基因背景亲本中棉所49为对照,研究EPSPS外源基因导入对陆地棉农艺性状和纤维品质的影响,结果表明外源基因的导入对棉花纤维上半部平均长度、马克隆值、断裂伸长率和断裂比强度等品质性状有显著影响;对于棉花衣分和铃重等农艺性状亦有显著的影响,衣分变异范围为30.5 %~42.7 %,T1和T2相关性分析表明衣分的变异为可遗传性变异,铃重最高为6.5 g,最低为4.6 g,两者相差40 %。因此,转基因棉花育种研究不仅要注重目标性状的转育,还要注重非目标性状的筛选。

关键词: 棉花; EPSPS; 外源基因; 农艺性状; 纤维品质

Abstract: The effects of an exogenous EPSPS gene on the agronomic traits and fiber quality of upland cotton were studied, using the transgenic glyphosate-resistant cotton germplasm lines G6-1 to G6-19 as materials and their non-transgenic genetic background cultivar, CCRI 49, as a control. The results showed that integration of the exogenous gene into cotton had a major impact on fiber quality factors, for instance fiber upper half mean length, micronaire, breaking elongation, and breaking tenacity, and also on agronomic traits such as lint and boll weight. The variation range of lint was 30.5%-42.7%, and lint variation was heritable according to the correlation analysis of T1 and T2. The maximum and minimum values of boll weight were 6.5 g and 4.6 g, respectively. These results suggested that the breeding of transgenic cotton should focus not only on goal-oriented characters but also non-target traits during selection.

Key words: cotton; EPSPS; exogenous gene; agronomic traits; fiber quality

中图分类号: 
  • S562.035