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棉花学报 ›› 2015, Vol. 27 ›› Issue (4): 291-299.doi: 10.11963/issn.1002-7807.201504001

• 研究与进展 •    下一篇

三个栽培种中天然彩色棉的遗传多样性及关联分析

许鸿越,孙君灵,潘兆娥,贾银华,庞保印,杜雄明*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳455000
  • 收稿日期:2014-04-10 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: dxm630723@163.com
  • 作者简介:许鸿越(1985―),男,硕士研究生,xhybl06@126.com
  • 基金资助:
    “十二五”国家支撑计划(2013BAD01B03、2011BAD35B05);农作物种质资源保护项目(NB2013-2130135)

Association Analysis and Genetic Diversity in Naturally Colored Cotton Varieties of Three Cultivated Species

Xu Hongyue, Sun Junling, Pan Zhaoe, Jia Yinhua, Pang Baoyin, Du Xiongming*   

  1. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, Henan  455000, China
  • Received:2014-04-10 Online:2015-07-15 Published:2015-07-15

摘要: 本研究利用主要农艺性状和SSR标记分析了137份陆地棉、13份海岛棉以及27份亚洲棉等不同纤维色泽的种质资源。170对SSR引物共检测到1036个等位基因变异。其中有多态性的等位基因923个,平均每个SSR位点有5.43个等位变异,变化范围为1~10。位点多态性信息量(PIC)的变化范围为0.56~0.93,PIC在0.85以上的多态性SSR位点为122个。结果表明,实验材料存在着丰富的遗传多样性。在群体结构分析基础上,使用TASSEL软件中的混合线性模型(MLM),在陆地棉中得到与11个性状相关联的SSR标记23个;在海岛棉群体得到与5个性状关联的17个标记;在亚洲棉中检测到与8个性状关联的15个标记位点。在三个栽培种中与纤维色泽度相关的标记位点一共有6个,分别是CIR51-4、BNL1421-2、BNL2656-2 、DPL570-2、GH268-6 和TMB131-1,表型变异解释率从3.12%到18.97%。三个栽培种中天然彩色棉种质具有丰富的遗传多样性,为棉花的种间、种内杂交育种以及拓宽棉花新品种的遗传背景提供了物质基础和理论依据。

关键词: 彩色棉; 遗传多样性; 关联分析; SSR标记

Abstract: Cotton germplasms with different fiber colors were analyzed using main agronomic traits and polymorphism microsatellite markers (SSR); these included 137 upland cotton (Gossypium hirsutum) varieties, 17 island cotton (Gossypium barbadense) varieties, and 27 Asiatic cotton (Gossypium arboreum) varieties. In total, we detected 1036 allelic variations, including 923 polymorphic alleles, with 170 primer pairs. Each SSR locus averaged 5.43 alleles with a variation range of 1 to 10. Locus polymorphism information content (PIC) ranged between 0.56 and 0.93, and there were 122 SSR loci with PIC>0.85. Used a mixed linear model with TASSEL software, 23 SSR markers associated with 11 traits in G. hirsutum, 17 markers associated with five traits in G. barbadense, and 15 markers associated with eight traits in G. arboreum. In the three cultivated species, six markers were associated with fiber color, including CIR51-4, BNL 1421-2, BNL2656-2, DPL570-2, GH268-6, and TMB131-1, with a phenotypic variation explanation rate ranging between 3.12% and 18.94%. Understanding the abundant genetic diversity exhibited by these three cultivated species is vital for future research on cotton interspecific- and intraspecific-hybridization breeding and the cultivation of new varieties.

Key words: colored cotton; genetic diversity; association analysis; SSR marker

中图分类号: 
  • S562.024