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棉花学报 ›› 2015, Vol. 27 ›› Issue (3): 208-215.doi: 10.11963/issn.1002-7807.201503003

• 研究与进展 • 上一篇    下一篇

盐胁迫下棉花K+和Na+离子转运的耐盐性生理机制

王宁,杨杰,黄群,苏桂兰,周红,许庆华,董合林,严根土*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室, 河南 安阳 455000
  • 收稿日期:2014-09-04 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: yangentu@163.com
  • 作者简介:王宁(1983― ),男,博士, wangning_4306202@aliyun.com
  • 基金资助:
    河南省现代农业产业技术体系项目(S2013-07-1);中央级公益性科研院所基本科研业务费专项资金(1610162014002)

Physiological Salinity Tolerance Mechanism for Transport of K+ and Na+ Ions in Cotton (Gossypium hirsutum L.) Seedlings under Salt Stress

Wang Ning, Yang Jie, Huang Qun, Su Guilan, Zhou Hong, Xu Qinghua, Dong Helin, Yan Gentu*   

  1. State Key Laboratory of Cotton Biology/ Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000, China
  • Received:2014-09-04 Online:2015-05-15 Published:2015-05-15
  • Contact: yangentu@163.com

摘要: 为了探究棉花的耐盐机制,以中棉所49、中棉所35和中51504为材料,研究了盐胁迫对棉花幼苗的生长及K+/Na+平衡生理的影响。结果表明,150 mmol·L-1 NaCl处理对幼苗的生长具有明显抑制作用,降低了叶片的光合速率(Pn)、PSⅡ实际光量子产额(ΦPSII)和电子传递速率(ETR),增加了非光化学荧光猝灭系数(qN)。与中棉所49和中棉所35相比,中51504的干物质累积受盐胁迫影响最小,且保持较高的Pn、ΦPSII、ETR和 qN值及较低的ETR/Pn值。盐胁迫提高了棉花组织中Na+的浓度,降低了K+的浓度;但中51504组织中保持了相对较低的Na+浓度和较高的K+浓度,维持了较高的K+/Na+比;通过非损伤微测技术(NMT)测定的离子流结果也表明,中51504的根系对Na+有较强的外排能力,而对K+有较强的保留和向地上部转运能力。能够有效地调节Na+和K+的跨膜转运进而维持K+/Na+平衡是棉花耐盐的重要生理机制之一。

关键词: 棉花; 盐胁迫; 耐盐性; 离子流; Na+/K+离子动态

Abstract: Salinity stress is one of the major abiotic factors affecting the growth and productivity of cotton(Gossypium hirsutum L.). To further understand the possible mechanism of salt tolerance, an experiment was conducted to investigate the effect of salt stress on growth and K+/Na+ homeostasis of cotton seedlings using three cultivars(CCRI 49, CCRI 35 and Zhong 51504). We found that 150 mmol·L-1 NaCl treatment significantly decreased biomass accumulation, photosynthesis rate(Pn), photochemical quenching(qP), photochemical quantum yield of photosystem (ΦPSII) and electron transport rate(ETR), but significantly increased non-photochemical quenching coefficients(qN) in PSII. The biomass accumulation of Zhong 51504 was less affected by salt stress than CCRI 49 or CCRI 35, and Zhong 51504 also retained higher Pn, ΦPSII, ETR and qN but lower ETR/Pn under saline conditions. Compared with the control, the salinity treatment caused a significant rise in Na+ concentration and decreased K+ concentration. The limited negative impact in Zhong 51504 derived from the lower content of Na+ and higher K+ in the tissues under salt stress. As expected, Zhong 51504 also retained a higher Na+/K+ ratio than other cultivars under salt treatment. Experiments of salt shock and long exposure to salt tested by the non-invasive ion flux measuring technique also revealed that Zhong 51504 not only had higher Na+ exclusion but also showed better K+ retention. Our results indicated that maintaining the K+/Na+ homeostasis can contribute to genotypic differences in salinity tolerance under conditions of high salinity.

Key words: cotton; salt stress; salt tolerance; ion flux; K+/Na+ homeostasis

中图分类号: 
  • S562.01