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棉花学报 ›› 2015, Vol. 27 ›› Issue (3): 189-197.doi: 10.11963/issn.1002-7807.201503001

• 研究与进展 •    下一篇

棉花C2H2类型锌指蛋白基因GhSIZ1的克隆及表达分析

张新宇,林书岱,张涛,裴柳玲,唐清,刘峰,刘永昌*   

  1. 石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆 石河子 832000
  • 收稿日期:2014-09-03 修回日期:2009-05-18 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: liuyongchang2003@126.com
  • 作者简介:张新宇(1978―),男,在读博士,zhxy@shzu.edu.cn
  • 基金资助:
    新疆生产建设兵团博士资金专项(2013BB003);石河子大学高层次人才启动项目(RCZX201218);石河子大学育种专项(2011gxjs-yz06)

Cloning and Expression Analysis of GhSIZ1, Encoding a C2H2 Zinc Finger Protein in Cotton(Gossypium hirsutum)

Zhang Xinyu, Lin Shudai, Zhang Tao, Pei Liuling, Tang Qing, Liu Feng, Liu Yongchang*   

  1. College of Agronomy, Shihezi University/ Key Oasis Eco-Agriculture Laboratory of Production and Construction Group, Shihezi, Xinjiang 832000, China
  • Received:2014-09-03 Revised:2009-05-18 Online:2015-05-15 Published:2015-05-15
  • Contact: liuyongchang2003@126.com

摘要: 通过RT-PCR 技术从陆地棉中克隆到1 个编码C2H2 型锌指蛋白的基因,命名为GhSIZ1(Stress Induced Zinc finger protein1)。该基因编码239个氨基酸残基,预测蛋白分子量为26.6205 kD,等电点为6.52。GhSIZ1包含一个C2H2 型锌指结构域,在其N端含有保守的L-box,C–末端含有一个转录抑制结构域EAR/DLN-box。系统发育树分析表明GhSIZ1与可可(XP_007044496.1)和中粒咖啡(CDP00218.1)中的锌指蛋白亲缘关系较近。实时定量PCR 分析GhSIZ1在棉花各组织中均有表达,但在根中表达量最高。低温、高盐、干旱胁迫处理后GhSIZ1的表达水平显著上调。亚细胞定位分析表明,GhSIZ1定位在细胞核中。本研究表明,GhSIZ1可能在棉花响应低温、高盐、干旱等非生物胁迫中起着重要作用。

关键词: 棉花; C2H2型锌指蛋白; 表达模式; GhSIZ1

Abstract: In this study, a novel gene encoding C2H2-type zinc finger protein was cloned in cotton by reverse-transcription polymerase chain reaction (PCR). The gene was designated as GhSIZ1 (Stress-induced Zinc finger protein1). GhSIZ1 encoded a protein of 239 amino acid residues with a predicted molecular mass of 26.6205 kD and an isoelectric point of 6.52. It has a C2H2-type zinc finger domain, an L-box at the N terminus and a putative transcription repression domain(EAR/DLN-box) at the C-terminus. Phylogenetic analysis revealed that GhSIZ1 shares high identity with C2H2-type zinc finger proteins from Theobroma cacao(XP_007044496.1) and Coffea canephora(CDP00218.1). Real-time PCR analysis showed that the transcripts of GhSIZ1 were detected in all tissues: roots, stems, flowers, leaves and fibers. The highest level was found in roots. The expression was induced by cold, salt or drought treatments. Subcellular localization analysis manifested that GhSIZ1 was distributed in the nucleus. Our results suggest that GhSIZ1 may play an important role in the response of cotton to cold, salt or drought.

Key words: cotton; C2H2-type zinc finger protein; expression pattern; GhSIZ1

中图分类号: 
  • S562.035