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棉花学报 ›› 2021, Vol. 33 ›› Issue (6): 493-503.doi: 10.11963/cs20210054

• 化学调控 • 上一篇    下一篇

甲哌鎓通过调节棉花叶片水分平衡和光合性能提高苗期耐旱性的生理机制

王玉贤#(),董莹莹#(),李芳军*(),杜明伟,田晓莉,李召虎   

  1. 中国农业大学农学院/作物化控研究中心/植物生长调节剂教育部工程研究中心,北京 100193
  • 收稿日期:2021-08-20 出版日期:2021-11-15 发布日期:2022-04-14
  • 通讯作者: 李芳军 E-mail:1457820274@qq.com;947893034@qq.com;lifangjun@cau.edu.cn
  • 作者简介:王玉贤(1996―),女,硕士, 1457820274@qq.com;|董莹莹, 947893034@qq.com
  • 基金资助:
    国家重点研发计划(2018YFD1000900)

Mepiquat chloride improves drought tolerance by adjusting water balance and photosynthetic performance in cotton seedling

Wang Yuxian#(),Dong Yingying#(),Li Fangjun*(),Du Mingwei,Tian Xiaoli,Li Zhaohu   

  1. Crop Chemical Control Research Center/College of Agronomy and Biotechnology, China Agricultural University/Engineering Research Center of Plant Growth Regulator, Ministry of Education, Beijing 100193, China
  • Received:2021-08-20 Online:2021-11-15 Published:2022-04-14
  • Contact: Li Fangjun E-mail:1457820274@qq.com;947893034@qq.com;lifangjun@cau.edu.cn

摘要:

【目的】研究植物生长延缓剂甲哌鎓 (Mepiquat chloride,MC/DPC)提高棉花幼苗耐旱性的调控机制。【方法】本研究以陆地棉品种中棉所41为供试材料,设CK-Ctrl(正常供水+喷施清水)、CK-DPC(正常供水+喷施30 mg·L-1 DPC)、DS-Ctrl(干旱+喷施清水)和DS-DPC(干旱+喷施30 mg·L-1 DPC)共4个处理。于2叶期叶面喷施DPC或清水,5 d后进行控水干旱处理,以正常供水为对照。【结果】正常供水条件下,叶面喷施DPC对叶片相对含水量、总叶绿素含量、净光合速率和水分利用率均无显著影响。干旱胁迫条件下,叶面喷施DPC可显著增加叶片中脯氨酸的积累、叶片相对含水量;DPC处理的叶片气孔导度和蒸腾速率虽呈增加趋势,但因净光合速率的显著提高最终增加了水分利用率。此外,DPC处理还可促进叶片可溶性蛋白的积累以抵御干旱胁迫。【结论】叶面喷施DPC可显著提高干旱胁迫条件下棉花幼苗的相对含水量、净光合速率和水分利用率,通过调节水分平衡和光合性能增强棉花抵御干旱胁迫的能力。

关键词: 棉花; 甲哌鎓; 耐旱; 保水; 光合作用

Abstract:

[Objective] The aim of this research is to study the regulation mechanism of plant growth regulator mepiquat chloride (MC/DPC) in improving the drought tolerance of cotton seedlings. [Method] In this study, CCRI 41(Gossypium hirsutum L.) was used as the test material, and four treatments including CK-Ctrl (sufficient water supply + spraying water), CK-DPC (sufficient water supply + spraying 30 mg·L-1 DPC), DS-Ctrl (drought stress + spraying water), and DS-DPC (drought stress + spraying 30 mg·L-1 DPC) were performed. At the two-leaf stage, DPC or water was sprayed on leaves, and then irrigation was stopped at five days as drought treatment, and using sufficient water supply as the control. [Result] The experimental results showed that under sufficient water conditions, DPC has no significant effect on the relative water content, total chlorophyll content, net photosynthetic rate, and water use efficiency of leaves. Under drought stress, DPC can significantly increase the accumulation of proline and relative water content. Meanwhile, DPC can slightly enhance the stomatal conductance and transpiration rate of leaves, and significantly increase the net photosynthetic rate, to improve the water use efficiency finally. In addition, DPC can also promote the accumulation of soluble protein of leaves in response to drought stress. [Conclusion] Spraying DPC can significantly increase the relative water content, net photosynthetic rate and water use efficiency of cotton seedlings, and can enhance the ability of cotton to resist drought stress by adjusting water balance and photosynthetic performance.

Key words: cotton; mepiquat chloride; drought tolerance; water retention; photosynthesis