甲哌鎓通过调节棉花叶片水分平衡和光合性能提高苗期耐旱性的生理机制

doi:10.11963/cs20210054

摘要/Abstract

摘要：

【目的】研究植物生长延缓剂甲哌鎓（Mepiquat chloride,MC/DPC）提高棉花幼苗耐旱性的调控机制。【方法】本研究以陆地棉品种中棉所41为供试材料,设CK-Ctrl（正常供水+喷施清水）、CK-DPC（正常供水+喷施30 mg·L^-1 DPC）、DS-Ctrl（干旱+喷施清水）和DS-DPC（干旱+喷施30 mg·L^-1 DPC）共4个处理。于2叶期叶面喷施DPC或清水,5 d后进行控水干旱处理,以正常供水为对照。【结果】正常供水条件下,叶面喷施DPC对叶片相对含水量、总叶绿素含量、净光合速率和水分利用率均无显著影响。干旱胁迫条件下,叶面喷施DPC可显著增加叶片中脯氨酸的积累、叶片相对含水量;DPC处理的叶片气孔导度和蒸腾速率虽呈增加趋势,但因净光合速率的显著提高最终增加了水分利用率。此外,DPC处理还可促进叶片可溶性蛋白的积累以抵御干旱胁迫。【结论】叶面喷施DPC可显著提高干旱胁迫条件下棉花幼苗的相对含水量、净光合速率和水分利用率,通过调节水分平衡和光合性能增强棉花抵御干旱胁迫的能力。

关键词: 棉花; 甲哌鎓; 耐旱; 保水; 光合作用

Abstract:

[Objective] The aim of this research is to study the regulation mechanism of plant growth regulator mepiquat chloride (MC/DPC) in improving the drought tolerance of cotton seedlings. [Method] In this study, CCRI 41(Gossypium hirsutum L.) was used as the test material, and four treatments including CK-Ctrl (sufficient water supply + spraying water), CK-DPC (sufficient water supply + spraying 30 mg·L^-1 DPC), DS-Ctrl (drought stress + spraying water), and DS-DPC (drought stress + spraying 30 mg·L^-1 DPC) were performed. At the two-leaf stage, DPC or water was sprayed on leaves, and then irrigation was stopped at five days as drought treatment, and using sufficient water supply as the control. [Result] The experimental results showed that under sufficient water conditions, DPC has no significant effect on the relative water content, total chlorophyll content, net photosynthetic rate, and water use efficiency of leaves. Under drought stress, DPC can significantly increase the accumulation of proline and relative water content. Meanwhile, DPC can slightly enhance the stomatal conductance and transpiration rate of leaves, and significantly increase the net photosynthetic rate, to improve the water use efficiency finally. In addition, DPC can also promote the accumulation of soluble protein of leaves in response to drought stress. [Conclusion] Spraying DPC can significantly increase the relative water content, net photosynthetic rate and water use efficiency of cotton seedlings, and can enhance the ability of cotton to resist drought stress by adjusting water balance and photosynthetic performance.

Key words: cotton; mepiquat chloride; drought tolerance; water retention; photosynthesis

王玉贤, 董莹莹, 李芳军, 杜明伟, 田晓莉, 李召虎. 甲哌鎓通过调节棉花叶片水分平衡和光合性能提高苗期耐旱性的生理机制[J]. 棉花学报, 2021, 33(6): 493-503.

Wang Yuxian, Dong Yingying, Li Fangjun, Du Mingwei, Tian Xiaoli, Li Zhaohu. Mepiquat chloride improves drought tolerance by adjusting water balance and photosynthetic performance in cotton seedling[J]. Cotton Science, 2021, 33(6): 493-503.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/cs20210054

http://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2021/V33/I6/493

图/表 5

图1

图2

图3

图4

图5

参考文献 48

[1]	Ashraf M, Foolad M R. Roles of glycine betaine and proline in improving plant abiotic stress resistance[J/OL]. Environmental and Experimental Botany, 2005, 59(2): 206-216[2021-08-10]. https://doi.org/10.1016/j.envexpbot.2005.12.006. doi: 10.1016/j.envexpbot.2005.12.006
[2]	Blokhina O, Virolainen E, Fagerstedt K V. Antioxidants, oxidative damage and oxygen deprivation stress: a review[J/OL]. Annals of Botany, 2003, 91(2): 179-194 [2021-08-10]. https://doi.org/10.1093/aob/mcf118. doi: 10.1093/aob/mcf118
[3]	周桂, 李杨瑞. 植物干旱诱导蛋白研究进展[J]. 广西农业科学, 2007, 38(4): 379-385.
	Zhou Gui, Li Yangrui. Advances in drought induced proteins in plants[J]. Guangxi Agricultural Sciences, 2007, 38(4): 379-385.
[4]	Farooq M, Wahid A, Kobayashi N, et al. Plant drought stress: effects, mechanisms and management[J/OL]. Agronomy for Sustainable Development, 2009, 29(1): 153-188[2021-08-10]. https://doi.org/10.1051/agro:2008021.
[5]	高宏云, 李军宏, 王远远, 等. 2个不同耐旱性棉花品种光合特性和干物质累积对干旱的响应[J/OL]. 新疆农业科学, 2020, 57(2): 233-244[2021-08-10]. https://doi.org/10.6048/j.issn.1001-4330.2020.02.004. doi: 10.6048/j.issn.1001-4330.2020.02.004
	Gao Hongyun, Li Junhong, Wang Yuanyuan, et al. Response of photosynthetic characteristics and dry matter accumulation to drought of two cotton varieties with different drought resistance[J/OL]. Xinjiang Agricultural Sciences, 2020, 57(2): 233-244[2021-08-10]. https://doi.org/10.6048/j.issn.1001-4330.2020.02.004. doi: 10.6048/j.issn.1001-4330.2020.02.004
[6]	Wang B M, Li Z X, Ran Q J, et al. ZmNF-YB16 overexpression improves drought resistance and yield by enhancing photosynthesis and the antioxidant capacity of maize plants[J/OL]. Frontiers in Plant Science, 2018, 9: 709-722[2021-08-10]. https://doi.org/10.3389/fpls.2018.00709. doi: 10.3389/fpls.2018.00709
[7]	安汶铠, 常丹, 张富春. 喷施2, 4-表油菜素内酯(EBR)提高棉花抗旱生理响应性的研究[J]. 基因组学与应用生物学, 2015, 34(10): 2217-2224.
	An Wenkai, Chang Dan, Zhang Fuchun. Improving the physiological response of cotton by spraying 2, 4-epibrassinolide (EBR) under the drought[J]. Genomics and Applied Biology, 2015, 34(10): 2217-2224.
[8]	杨艺, 常丹, 王艳, 等. 茉莉酸甲酯对棉花抗旱效果的影响[J/OL]. 西北农业学报, 2016, 25(9): 1333-1341[2021-08-10]. https://doi.org/10.7606/j.issn.1004-1389.2016.09.009.
	Yang Yi, Chang Dan, Wang Yan, et al. Effect of methyl jasmonate (MeJA) on enhancing drought resistance of cotton[J/OL]. Acta Agriculturae Boreali-Occidentalis Sinica, 2016, 25(9): 1333-1341[2021-08-10]. https://doi.org/10.7606/j.issn.1004-1389.2016.09.009.
[9]	张茜, 康西璐, 顾文哲, 等. 外源一氧化氮对干旱和盐胁迫下棉种萌发的影响[J/OL]. 中国棉花, 2016, 43(12): 11-15, 20[2021-08-10]. https://doi.org/10.11963/issn.1000-632X.201612003.
	Zhang Xi, Kang Xilu, Gu Wenzhe, et al. Effects of exogenous nitric oxide on the seed germination of cotton (Gossypium hirsutum L.) under drought and salt stresses[J/OL]. China Cotton, 2016, 43(12): 11-15, 20[2021-08-10]. https://doi.org/10.11963/issn.1000-632X.201612003.
[10]	何钟佩, 杨秉芳. 缩节安对棉花生长发育的调控[J]. 植物杂志, 1983(3): 10-12.
	He Zhongpei, Yang Bingfang. Regulation of mepiquat chloride on cotton growth and development[J]. The Plant Journal, 1983(3): 10-12.
[11]	王丽, 张明才, 杜明伟, 等. 赤霉素生物合成酶基因GhCPS和GhKS参与甲哌鎓对棉花幼苗叶片生长的控制[J/OL]. 作物学报, 2014, 40(8): 1350-1355[2021-08-10]. https://doi.org/10.3724/SP.J.1006.2014.01350. doi: 10.3724/SP.J.1006.2014.01350
	Wang Li, Zhang Mingcai, Du Mingwei, et al. GhCPS and GhKS encoding gibberellin biosynthesis enzymes involve in inhibition of leaf growth by mepiquat chloride in cotton (Gossypium hirsutum L.)[J/OL]. Acta Agronomica Sinica, 2014, 40(8): 1350-1355[2021-08-10]. https://doi.org/10.3724/SP.J.1006.2014.01350. doi: 10.3724/SP.J.1006.2014.01350
[12]	Xu X, Taylor H M. Increase in drought resistance of cotton seedlings treated with mepiquat chloride[J/OL]. Agronomy Journal, 1992, 84: 569-574[2021-08-10]. https://doi.org/10.2134/agronj1992.00021962008400040005x. doi: 10.2134/agronj1992.00021962008400040005x
[13]	段留生, 黄志强, 于运华, 等. 缩节安(DPC)和黄腐酸(FA)对棉花种子萌发和幼苗素质的影响[J]. 莱阳农学院学报, 1997, 14(2): 33-37.
	Duan Liusheng, Huang Zhiqiang, Yu Yunhua, et al. Effects of DPC and FA on seed germination and seedling quality of cotton[J]. Journal of Laiyang Agricultural College, 1997, 14(2): 33-37.
[14]	李秀菊, 职明星, 何兴武, 等. 缩节安对无毒棉和抗虫棉抗旱性生理效应研究[J]. 河南职业技术师范学院学报, 1999, 27(3): 30-33.
	Li Xiuju, Zhi Mingxing, He Xingwu, et al. Effects of DPC on the drought resistance of cotton seedlings[J]. Journal of Henan Vocation-Technical Teachers College, 1999, 27(3): 30-33.
[15]	王宁, 田晓莉, 段留生, 等. 缩节胺浸种提高棉花幼苗根系活力中的活性氧代谢[J/OL]. 作物学报, 2014, 40(7): 1220-1226[2021-08-10]. https://doi.org/10.3724/SP.J.1006.2014.01220.
	Wang Ning, Tian Xiaoli, Duan Liusheng, et al. Metabolism of reactive oxygen species involved in increasing root vigor of cotton seedlings by soaking seeds with mepiquat chloride[J/OL]. Acta Agronomica Sinica, 2014, 40(7): 1220-1226[2021-08-10]. https://doi.org/10.3724/SP.J.1006.2014.01220.
[16]	陈志峰, 王海永, 董学会. DPC拌种对玉米幼苗形态和抗旱性的影响[J]. 玉米科学, 2011, 19(5): 78-82.
	Chen Zhifeng, Wang Haiyong, Dong Xuehui. Effects of seed dressing by DPC on maize seedling shape and drought resistance[J]. Journal of Maize Sciences, 2011, 19(5): 78-82.
[17]	张西岭, 杨异超, 朱荷琴. 缩节安(DPC)浸种浓度和时间对棉籽发芽及幼苗生长的影响[J]. 陕西农业科学, 1994(3): 8-10.
	Zhang Xiling, Yang Yichao, Zhu Heqin. Effects of DPC concentration and time on seed germination and seedling growth[J]. Shaanxi Journal of Agricultural Sciences, 1994(3): 8-10.
[18]	周运刚, 王俊刚, 马天文, 等. 不同DPC(缩节胺)处理对棉花生理生化特性的影响[J/OL]. 新疆农业科学, 2010, 47(6): 1142-1146[2021-08-10]. .
	Zhou Yungang, Wang Jungang, Ma Tianwen, et al. Influences of different DPC treatments on physiological and biochemical characteristics of cotton[J/OL]. Xinjiang Agricultural Sciences, 2010, 47(6): 1142-1146[2021-08-10]. .
[19]	李合生. 植物生理生化原理和技术[M]. 北京: 高等教育出版社, 2000: 164-165.
	Li Hesheng. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000: 164-165.
[20]	孔祥生, 易现锋. 植物生理学实验技术[M]. 北京: 中国农业出版社, 2008: 132-250.
	Kong Xiangsheng, Yi Xianfeng. The experimental skill in plants physiology[M]. Beijing: China Agriculture Press, 2008: 132-250.
[21]	陶汉之, 李展, 孟彩萍, 等. 对生玉米叶面积分布、蒸腾速率和水分利用率的研究[J/OL]. 作物学报, 2000, 26(1): 65-70[2021-08-10]. .
	Tao Hanzhi, Li Zhan, Meng Caiping, et al. Studies on distribution of leave area, transpiration rate and water use efficiency in oppositifolious maize[J/OL]. Acta Agronomica Sinica, 2000, 26(1): 65-70[2021-08-10]. .
[22]	Zhou H M, Zhang F C, Roger K, et al. Peach yield and fruit quality is maintained under mild deficit irrigation in semi-arid China[J/OL]. Journal of Integrative Agriculture, 2016, 16(5): 1173-1183[2021-08-10]. https://doi.org/10.1016/S2095-3119(16)61571-X. doi: 10.1016/S2095-3119(16)61571-X
[23]	Saleem M F, Raza M A S, Ahmad S, et al. Understanding and mitigating the impacts of drought stress in cotton-a review[J/OL]. Pakistan Journal of Agricultural Sciences, 2016, 53(3): 1-15[2021-08-10]. https://doi.org/10.21162/PAKJAS/16.3341. doi: 10.21162/PAKJAS
[24]	Dabbert T A. Gore M A. Challenges and perspectives on improving heat and drought stress resilience in cotton[J]. Journal of Cotton Science, 2014, 18(3): 393-409.
[25]	Niakan M, Habibi A. Effect of pix regulator on vegetative growth of cotton plant[J]. Annals of Biological Research, 2013, 4(1): 53-58.
[26]	Wang L, Mu C, Du M, et al. The effect of mepiquat chloride on elongation of cotton (Gossypium hirsutum L.) internode is associated with low concentration of gibberellic acid[J/OL]. Plant Science, 2014, 225: 15-23[2021-08-10]. https://doi.org/10.1016/j.plantsci.2014.05.005. doi: 10.1016/j.plantsci.2014.05.005 pmid: 25017155
[27]	Zhang J, Zhang Y S, Xing J P, et al. Introducing selective agrochemical manipulation of gibberellin metabolism into a cereal crop[J/OL]. Nature Plants, 2020, 6: 67-72[2021-08-10]. https://doi.org/10.1038/s41477-019-0582-x. doi: 10.1038/s41477-019-0582-x pmid: 32015514
[28]	Xie Z, Nolan T, Jiang H, et al. The AP2/ERF transcription factor tiny modulates brassinosteroid-regulated plant growth and drought responses in Arabidopsis[J/OL]. The Plant Cell, 2019, 31(8): 1788-1806[2021-08-10]. https://doi.org/10.1105/tpc.18.00918. doi: 10.1105/tpc.18.00918
[29]	李永山, 张凯, 王晓璐, 等. 陆地棉品种根系特性与耐旱性关系的研究[J]. 棉花学报, 2000, 12(2): 85-86.
	Li Yongshan, Zhang Kai, Wang Xiaolu, et al. Studies on relationships between root characters and drought tolerance in upland cotton[J]. Cotton Science, 2000, 12(2): 85-86.
[30]	Wu Q, Du M W, Wu J, et al. Mepiquat chloride promotes cotton lateral root formation by modulating plant hormone homeostasis[J/OL]. BMC Plant Biology, 2019, 19(1): 573-588[2021-08-10]. https://doi.org/10.1186/s12870-019-2176-1. doi: 10.1186/s12870-019-2176-1
[31]	de Almeida A Q, Rosolem C A. Cotton root and shoot growth as affected by application of mepiquat chloride to cotton seeds[J]. Acta Scientiarum Agronomy, 2012, 34(1): 61-65.
[32]	Deeba F, Pandey A, Ranjan S, et al. Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress[J/OL]. Plant Physiology and Biochemistry, 2012, 53: 6-18[2021-08-10]. https://doi.org/10.1016/j.plaphy.2012.01.002. doi: 10.1016/j.plaphy.2012.01.002
[33]	王凤宝, 董立峰, 尹秀玲, 等. 小麦离体叶片室内自然干化失水率与品种抗旱性关系的研究[J]. 河北农业技术师范学院学报, 1997, 11(1): 53-57.
	Wang Fengbao, Dong Lifeng, Yin Xiuling, et al. Study on the relationship between variety drought resistance and water losing rate of isolated wheat leaves dried under the natural indoor condition[J]. Journal of Hebei Agrotechnical Teachers College, 1997, 11(1): 53-57.
[34]	Lv S, Yang A, Zhang K, et al. Increase of glycine betaine synthesis improves drought tolerance in cotton[J]. Molecular Breeding, 2007, 20: 233-248. doi: 10.1007/s11032-007-9086-x
[35]	陈志成, 王志伟, 王荣荣, 等. 3种阔叶树种对持续干旱的生理响应及抗旱性评价[J]. 中国水土保持科学, 2013, 11(2): 65-71.
	Chen Zhicheng, Wang Zhiwei, Wang Rongrong, et al. Physiological response of three broadleaved tree species to drought stress and evaluation of drought resistance[J]. Science of Soil and Water Conservation, 2013, 11(2): 65-71.
[36]	马艳丽, 王鹏. 植物在干旱中的适应机制研究进展[J]. 河北林果研究, 2010, 25(4): 359-361.
	Ma Yanli, Wang Peng. Research progresses of plant adaptation mechanism to drought stress[J]. Hebei Journal of Forestry and Orchcard Research, 2010, 25(4): 359-361.
[37]	田又升, 王志军, 于航, 等. 干旱胁迫对不同抗旱性棉花品种抗氧化酶活性及基因表达的影响[J]. 西北植物学报, 2015, 35(12): 2483-2490.
	Tian Yousheng, Wang Zhijun, Yu Hang, et al. Response of antioxidant enzyme activities and gene expression in different drought resistance cotton varieties under drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(12): 2483-2490.
[38]	王一, 曹敏建, 李春红, 等. 模拟干旱对不同耐性玉米自交系幼苗根系和水分利用效率的影响[J/OL]. 作物杂志, 2011(6): 50-52[2021-08-10]. .
	Wang Yi, Cao Minjian, Li Chunhong, et al. Effects of simulated drought on root and WUE of maize inbred lines with different tolerance[J/OL]. Crops, 2011(6): 50-52[2021-08-10]. .
[39]	Li Y P, Li H B, Li Y Y, et al. Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat[J/OL]. Crop Journal, 2017, 5(3): 231-239[2021-08-10]. https://doi.org/10.1016/j.cj.2017.01.001. doi: 10.1016/j.cj.2017.01.001
[40]	Patil M D, Biradar D P, Patil V C, et al. Response of cotton genotypes to drought mitigation practices[J]. American-Eurasian Journal of Agricultural & Environmental Sciences, 2011, 11(3): 360-364.
[41]	Flexas J, Bota J, Loreto J, et al. Diffusive and metabolic limitations to photosynthesis under drought and salinity in C₃ plants[J/OL]. Plant Biology, 2004, 6: 269-279[2021-08-10]. https://doi.org/10.1055/s-2004-820867. pmid: 15143435
[42]	刘铨义, 周晶, 王文博, 等. 缩节胺对棉花品种生理生育特性影响研究[J/OL]. 新疆农业科学, 2015, 52(7): 1280-1284[2021-08-10]. .
	Liu Quanyi, Zhou Jing, Wang Wenbo, et al. Study the effect of DPC treatment on physiological and fertility characteristics of cotton[J/OL]. Xinjiang Agricultural Sciences, 2015, 52(7): 1280-1284[2021-08-10]. .
[43]	崔家丽, 王俊刚. 甲哌鎓对棉花叶片光合生理及蜡质含量的影响[J]. 农药学学报, 2017, 19(3): 393-397.
	Cui Jiali, Wang Jungang. Effects of mepiquat chloride on photosynthetic physiology and wax content of cotton leaves[J]. Journal of Pesticide Science, 2017, 19(3): 393-397.
[44]	许大全. 光合作用气孔限制分析中的一些问题[J]. 植物生理学通讯, 1997, 33(4): 241-244.
	Xu Daquan. Some problems in stomatal limitation analysis of photosynthesis[J]. Plant Physiology Communications, 1997, 33(4): 241-244.
[45]	卢碧霞, 张改生, 夏勉, 等. 水稻rbcS启动子的克隆及其在转基因水稻中的特异性表达[J]. 西北农林科技大学学报(自然科学版), 2007, 35(1): 96-100, 105.
	Lu Bixia, Zhang Gaisheng, Xia Mian, et al. Cloning of the promoter of rice rbcS and its specific expression in transgenic rice[J]. Journal of Northwest A & F University (Natural Science Edition), 2007, 35(1): 96-100, 105.
[46]	Chakrabortee S, Boschetti C, Walton L J, et al. Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function[J/OL]. Proceedings of The National Academy of Sciences of The United States of America, 2007, 104(46): 18073-18078[2021-08-10]. https://doi.org/10.1073/pnas.0706964104. doi: 10.1073/pnas.0706964104 pmid: 17984052
[47]	Deeba F, Pandey A K, Ranjan S, et al. Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress[J/OL]. Plant Physiology and Biochemistry, 2012, 53: 6-18[2021-08-10]. https://doi.org/10.1016/j.plaphy.2012.01.002. doi: 10.1016/j.plaphy.2012.01.002
[48]	Kosova K, Vitamvas P, Prasil I T, et al. Plant proteome changes under abiotic stress-contribution of proteomics studies to understanding plant stress response[J/OL]. Journal of Proteomics, 2011, 74(8): 1301-1322[2021-08-10]. https://doi.org/10.1016/j.jprot.2011.02.006. doi: 10.1016/j.jprot.2011.02.006