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棉花学报 ›› 2021, Vol. 33 ›› Issue (6): 482-492.doi: 10.11963/cs20210036

• 研究与进展 • 上一篇    下一篇

基于广泛靶向代谢组学的不同颜色棉花花瓣中类黄酮成分差异分析

李秋琳(),李燕,陈伟,姚金波,朱守鸿,袁黎,张永山*()   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2021-06-04 出版日期:2021-11-15 发布日期:2022-04-14
  • 通讯作者: 张永山 E-mail:liqiulin1214@163.com;13938698299@163.com
  • 作者简介:李秋琳(1995―),女,硕士研究生, liqiulin1214@163.com
  • 基金资助:
    国家重点研发计划(2017YFD0101600)

Metabolomics reveals the variation of flavonoids content in petals of cotton with different colors

Li Qiulin(),Li Yan,Chen Wei,Yao Jinbo,Zhu Shouhong,Yuan Li,Zhang Yongshan*()   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/ State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2021-06-04 Online:2021-11-15 Published:2022-04-14
  • Contact: Zhang Yongshan E-mail:liqiulin1214@163.com;13938698299@163.com

摘要:

【目的】本研究旨在对棉花黄色、乳白色和白色花瓣中类黄酮类代谢物质成分及差异进行分析,为进一步研究棉花花瓣颜色的形成机制和花瓣色素的利用提供一定的理论基础。【方法】用超高效液相色谱-串联质谱检测平台和Analyst 1.6.3软件对海7124(黄色花)、TM-1(乳白色花)和石系亚1号(白色花)3种花瓣中的类黄酮物质进行检测和分析;然后使用多元统计分析方法对检测到的类黄酮类代谢物数据进行分析,筛选差异代谢物;最后利用KEGG网站结合K-means分析对差异代谢物参与的合成途径进行分析。【结果】3种棉花花瓣材料中共检测到184种类黄酮类代谢物,其中黄酮醇类代谢物(76种,占41.30%)和黄酮类代谢物(51种,占27.72%)居多;差异代谢物171种,显著富集在黄酮和黄酮醇的生物合成途径及类黄酮的生物合成途径。通过对黄色、乳白色、白色花瓣进行类黄酮类代谢物分析,发现差异代谢物含量呈升高趋势和降低趋势的物质各15种,其中被注释到KEGG通路上的有5种:在黄酮和黄酮醇的生物合成途径上,金圣草黄素、紫云英苷和芦丁含量均升高;在类黄酮的生物合成途径上,香橙素和表儿茶素含量升高,异杞柳苷含量降低。【结论】通过分析发现3种棉花材料中存在的类黄酮类差异代谢物主要参与黄酮和黄酮醇的生物合成途径及类黄酮的生物合成途径;属于查耳酮类的异杞柳苷在黄色、乳白色、白色花瓣中的含量逐渐减少,推测该物质与棉花花瓣的黄色相关。

关键词: 棉花; 花瓣颜色; 代谢物; 类黄酮; UPLC-MS/MS; 异杞柳苷

Abstract:

[Objective] This study aims to analyze the composition and difference of flavonoid metabolites in yellow, milky white and white petals of cotton, which provides theoretical basis for the further research and utility of pigmentation in cotton flower. [Method] In this study, firstly, the Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection platform and Analyst 1.6.3 were employed to analyze the flavonoids in three types of cotton petals from Hai 7124(yellow flower), TM-1 (milk white flower), and Shixiya 1 (white flower). Then the multivariate statistical analysis was used to analyze different metabolites. Finally, KEGG and K-means was used to investigate the synthetic pathways of different metabolites. [Result] A total of 184 types of flavonoid metabolites were detected in petals of three colors, among which 76 were flavonol (accounting for 41.30%) and 51 were flavone (accounting for 27.72%). And 171 different metabolites were obtained, which were significantly enriched in the biosynthetic pathway of flavone and flavonols and the biosynthetic pathway of flavonoids. By analyzing the relative content of flavonoids metabolites in yellow, milky white and white petals, 15 different metabolites were found to show an increasing trend and 15 metabolites content were decreasing. Among them, 5 metabolites were annotated in the known KEGG pathway, and the content of chrysoeriol, astragalin and rutin were all increased in the biosynthesis pathway of flavone and flavonoid. In addition, in flavonoid biosynthesis pathway, the content of aromadendrin and epicatechin increased, and the content of isosalipurposide were decreased. [Conclusion] Different flavonoid metabolites in the three cotton materials were found to be mainly involved in the biosynthesis pathway of flavone and flavonol as well as the biosynthesis pathway of flavonoids. As the content of isoaridin was decreased gradually in yellow, milky white and white petals, we speculate that this substance was related to the formation of yellow cotton petals.

Key words: cotton; petal color; metabolites; flavonoids; UPLC-MS/MS; isosalipurposide