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棉花学报 ›› 2021, Vol. 33 ›› Issue (4): 328-336.doi: 10.11963/cs20210021

• 研究与进展 • 上一篇    下一篇

龟纹瓢虫生长发育及卵巢与滤泡上皮细胞的研究

皇甫宁博(),高雪珂,王丽,朱香镇,张开心,李东阳,姬继超(),雒珺瑜(),崔金杰   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2021-03-18 出版日期:2021-07-15 发布日期:2021-09-14
  • 通讯作者: 姬继超,雒珺瑜 E-mail:huangfuningbo@126.com;hnnydxjc@163.com;luojunyu1818@126.com
  • 作者简介:皇甫宁博(1994―),男,硕士研究生, huangfuningbo@126.com
  • 基金资助:
    中国农业科学院棉花研究所中央级公益性科研院所基本科研业务费专项(1610162021033)

Study on the development, oogenesis and follicular epithelial cell of Propylea japonica

Huangfu Ningbo(),Gao Xueke,Wang Li,Zhu Xiangzhen,Zhang Kaixin,Li Dongyang,Ji Jichao(),Luo Junyu(),Cui Jinjie   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2021-03-18 Online:2021-07-15 Published:2021-09-14
  • Contact: Ji Jichao,Luo Junyu E-mail:huangfuningbo@126.com;hnnydxjc@163.com;luojunyu1818@126.com

摘要:

【目的】 龟纹瓢虫是农田害虫的重要捕食性天敌。明确龟纹瓢虫生长发育、卵子发生和滤泡上皮细胞发育规律对于解决人工扩繁龟纹瓢虫的难题至关重要。【方法】 对龟纹瓢虫生长发育和生殖相关参数进行了测定,并通过细胞核和细胞骨架染色对龟纹瓢虫卵巢小管和滤泡上皮细胞形态进行系统观察。【结果】 (1)龟纹瓢虫1龄、2龄、3龄、4龄幼虫、蛹、雌成虫、雄成虫平均历期分别为1.13 d±0.09 d、1.48 d±0.11 d、1.34 d±0.08 d、2.34 d±0.06 d、2.81 d±0.08 d、51.08 d±1.13 d、48.36 d±1.29 d。在2~3龄幼虫期体长增加最快,4龄幼虫体长达到最长9.32 mm±0.27 mm,成虫体宽达到最宽3.70 mm±0.05 mm;(2)单雌日均产卵量最高为14.42粒±2.36粒,单雌累计产卵量为414粒±41.27粒,最高可达654粒。(3)明确了卵泡和卵母细胞动态变化,并观察到在卵黄沉积期滤泡上皮细胞间隙的形成,主要用于卵母细胞摄取卵黄原蛋白。【结论】 本研究明确了龟纹瓢虫体长、体宽和卵子发生规律、滤泡上皮细胞发育规律,为进一步探索龟纹瓢虫生殖调控分子机制、解决人工扩繁难题提供理论依据。

关键词: 龟纹瓢虫; 卵巢管; 滤泡上皮细胞; 细胞染色; 卵黄原蛋白

Abstract:

[Objective] The Propylea japonica is an important predator in farmland. It is very important to reveal the growth and development, the regularity of the development of oogenesis and follicular epithelial cell in P. japonica for solving the problem of artificial propagation of ladybirds. [Methode] The parameters related to the development and reproduction of P. japonica were measured. The morphology of ovarioles and follicular epithelial cells of P. japonica were systematically observed by staining of nucleus and cytoskeleton. [Result] (1) The results showed that the average duration of the 1st, 2nd, 3rd and 4th instar larvae, and pupa, female adults, and male adults of P. japonica were 1.13 d±0.09 d, 1.48 d±0.11 d, 1.34 d±0.08 d, 2.34 d±0.06 d, 2.81 d±0.08 d, 51.08 d±1.13 d and 48.36 d±1.29 d, respectively. The body length increased rapidly from the 2nd to the 3rd instars larvae. The larva reached the longest body length of 9.32 mm±0.27 mm at the 4th instar, and the body width reached the widest of 3.70 mm±0.05 mm in adult. (2) The highest daily average oviposition of a female was 14.42±2.36. The average number of eggs laid by a single female was 414±41.27, and the maximum number was 654. (3) The dynamic changes of follicles and oocytes were defined, and the formation of space (patency) between the follicular epithelial cells during yolk deposition phase was observed, which was mainly used for the uptake of vitellogenin by the oocytes. [Conclusion] In this study, we confirmed the regularity of body length, body width, oogenesis, and follicular epithelial cell development of P. japonica. This study provides a theoretical basis for us to further explore the molecular mechanism of reproductive regulation and to solve the problem of artificial propagation.

Key words: Propylea japonica; ovariole; follicular epithelial cell; cell stain; vitellogenin