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棉花学报 ›› 2021, Vol. 33 ›› Issue (4): 337-346.doi: 10.11963/cs20200065

• 研究与进展 • 上一篇    下一篇

棉花UGPase基因鉴定与生物信息学分析

张岚(),程琦(),梁士辰,邓雨潇,潘玉欣()   

  1. 华北理工大学生命科学学院,河北 唐山 063210
  • 收稿日期:2020-08-04 出版日期:2021-07-15 发布日期:2021-09-14
  • 通讯作者: 潘玉欣 E-mail:zhanglan1374@sohu.com;askjiaqi98@163;panyu-xin@163.com
  • 作者简介:张岚(1983―),女,研究生,讲师, zhanglan1374@sohu.com|程琦: askjiaqi98@163
  • 基金资助:
    河北省教育厅青年基金项目(QN2020139);华北理工大学大学生创新创业项目(X2019253)

Identification and bioinformatic analysis of UGPase gene family in cotton

Zhang Lan(),Cheng Qi(),Liang Shichen,Deng Yuxiao,Pan Yuxin()   

  1. College of Life Sciences, North China University of Science and Technology, Tangshan, Hebei 063210, China
  • Received:2020-08-04 Online:2021-07-15 Published:2021-09-14
  • Contact: Pan Yuxin E-mail:zhanglan1374@sohu.com;askjiaqi98@163;panyu-xin@163.com

摘要:

【目的】 尿苷二磷酸葡萄糖焦磷酸化酶(Uridine diphosphate glucose pyrophosphorylase,UGPase)是糖合成代谢途径中的一种重要限速酶,在植物纤维细胞发育中起重要作用,但是关于棉花中UGPase基因的生物信息学研究却很少。本研究采用生物信息学手段鉴定和分析棉花UGPase基因家族特性,为其相关研究提供重要线索。【方法】 以陆地棉、海岛棉、亚洲棉和雷蒙德氏棉4种棉花为主要研究对象,结合藻类、水稻、葡萄、可可、榴莲等19种植物的UGPase基因进行系统进化分析和陆地棉UGPase基因的表达特性分析。【结果】 系统发育分析显示UGPase基因被分为UGPase-A和UGPase-B两大类。保守基序及适应性进化分析显示,UGPase-B类基因在进化中十分保守,UGPase-A类基因在进化中与UGPase-B类基因存在较大差异。棉花、葡萄、可可、榴莲UGPase基因同源性分析显示,棉花中大多数UGPase基因来源于异源四倍体棉花形成之前的基因组加倍事件。UGPase-A类在棉纤维发育起始和伸长期表达量较高,UGPase-B类在营养器官中表达量较高。【结论】 本研究明确了UGPase两类基因的结构、功能与进化,为后续研究奠定基础。

关键词: 棉花; 糖代谢; 进化; 纤维发育; 功能分化; 表达特性分析

Abstract:

[Objective] Uridine diphosphate glucose pyrophosphorylase (UGPase) is an important rate-limiting enzyme involved in glucose anabolism, which plays an important role in the development of plant fiber cells. However, there is little research in cotton UGPase genes. This study aimed to identify and analyze the characteristics of members of UGPase gene family in cotton. [Methode] Gossypium hirsutum, G. barbadense, G. arboreum, and G. raimondii were selected as the main research objects. The phylogenetic and expression characteristics of UGPase genes in G. hirsutum were analyzed by combining UGPase genes from 19 kinds of plants, including algae, rice, grape, cocao, durian, and so on. [Result] Phylogenetic analysis showed that UGPase genes were divided into two groups, UGPase-A and UGPase-B. Conserved motif and adaptive evolution analysis showed that UGPase-B type genes were very conservative in evolution, while UGPase-A type genes had great differences with UGPase-B in evolution. Homology analysis of UGPase genes in cotton, grape, cocao and durian showed that most of cotton UGPase genes originated from duplication events before the formation of allotetraploid cotton. The expression levels of UGPase-A type genes were higher at the initiation and elongation stage of cotton fiber development, and the expression levels of UGPase-B type genes were higher in vegetative organs. [Conclusion] The structure, function and evolution of cotton UGPase genes were analyzed, which laid the foundation for further study.

Key words: cotton; carbohydrate metabolism; evolution; fiber development; functional differentiation; expression characteristic analysis