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棉花学报 ›› 2021, Vol. 33 ›› Issue (4): 368-376.doi: 10.11963/cs20200060

• 研究简报 • 上一篇    下一篇

FBP7::iaaM基因陆地棉育种应用初报

程成(),李斌,王雅丽,赵楠,苏莹,聂虎帅,华金平()   

  1. 中国农业大学农学院棉花遗传育种与基因组研究实验室/作物遗传改良北京市重点实验室/杂种优势利用教育部重点实验室,北京 100193
  • 收稿日期:2020-06-22 出版日期:2021-07-15 发布日期:2021-09-14
  • 通讯作者: 华金平 E-mail:b20183010016@cau.edu.cn;jinping_hua@cau.edu.cn
  • 作者简介:程成(1993―),男,博士研究生, b20183010016@cau.edu.cn
  • 基金资助:
    国家科技重大专项——转基因生物新品种培育(2016ZX08005)

Evaluation on the genetic effects of FBP7::iaaM gene in upland cotton

Cheng Cheng(),Li Bin,Wang Yali,Zhao Nan,Su Ying,Nie Hushuai,Hua Jinping()   

  1. Laboratory of Cotton Genetics, Genomics and Breeding, College of Agronomy and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education, China Agricultural University, Beijing 100193, China
  • Received:2020-06-22 Online:2021-07-15 Published:2021-09-14
  • Contact: Hua Jinping E-mail:b20183010016@cau.edu.cn;jinping_hua@cau.edu.cn

摘要:

【目的】 研究转FBP7::iaaM基因对不同陆地棉材料纤维产量、品质及种子活力的影响。【方法】 以转FBP7::iaaM基因种质IF1-1为父本,与中棉所12、鄂抗棉9号及其谱系涉及的亲本等27个不同遗传背景陆地棉的品种杂交获得F1,通过分子检测确定阳性植株,考种测定衣分、籽指,取样进行纤维品质检测和种子发芽试验,比较FBP7::iaaM基因效应,评价IF1-1的育种应用价值。【结果】 分子检测确认F1阳性植株,取样考种发现:与陆地棉母本比较,多数包含FBP7::iaaM基因的F1衣分提高(平均提高6.60百分点),籽指降低(平均降低1.8 g);F1纤维品质未表现出明显负效应:部分材料的马克隆值等级提高,纤维上半部平均长度、断裂比强度和断裂伸长率提高。发芽试验结果表明,尽管绝大多数包含FBP7::iaaM基因的F1种子籽指降低,但是发芽率和发芽势基本不变。【结论】 FBP7::iaaM基因转育合适的受体材料可以显著提高衣分,对种子发芽没有不利影响,IF1-1在同步改良棉花纤维品质和产量方面有重要的利用价值。

关键词: 陆地棉; FBP7::iaaM; 衣分; 籽指; 发芽率; 纤维品质

Abstract:

[Objective] The aim of this study is to evaluate the genetic effect of FBP7::iaaM gene on fiber yield, quality and seed vigor in different upland cotton varieties. [Methode] In this study, IF1-1 was used as a male parent to produce hybrids with 27 cotton varieties of different genetic backgrounds, which are CCRI 12 and Ekangmian No. 9 and their originated parent lines. The lint percentage, seed index, fiber quality traits, germination potential and seed germination rate of F1 were detected to evaluate the breeding value of FBP7::iaaM gene in IF1-1. [Result] The lint percentage of F1 hybrids with FBP7::iaaM gene increased by an average of 6.60 percentage points, and seed index decreased with an average of 1.8 g, and the FBP7::iaaM gene had no negative effect on seed germination potential and germination rate. Most F1 hybrids showed better fiber quality, such as fiber micronaire, fiber length, breaking tenacity, and breaking elongation. [Conclusion] In one word, IF1-1 germplasm line with FBP7::iaaM gene showed important potential in genetic improvement in upland cotton.

Key words: upland cotton; FBP7:iaaM; lint percentage; seed index; germination rate; fiber quality