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棉花学报 ›› 2019, Vol. 31 ›› Issue (1): 23-30.doi: 10.11963/1002-7807.zxfzxf.20181226

• 研究与进展 • 上一篇    下一篇

棉花导入系耐盐性鉴定及耐盐基因QTL定位

朱协飞(),司占峰   

  1. 南京农业大学作物遗传与种质创新国家重点实验室,南京 210095
  • 收稿日期:2017-09-07 发布日期:2019-01-15
  • 作者简介:朱协飞(1963―), fxx615@njau.edu.cn
  • 基金资助:
    国家重点研发计划(2016YFD0101403);国家现代农业产业技术体系棉花产业技术体系(CARS-18-01)

Evaluation and QTL Mapping of Tolerance to Salinity Using Interspecific Introgression Lines from Gossypium barbadense in G. hirsutum

Zhu Xiefei(),Si Zhanfeng   

  1. State laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2017-09-07 Published:2019-01-15

摘要:

【目的】筛选棉花耐盐、抗盐种质。【方法】以148份陆地棉背景的海岛棉染色体片断导入系为研究对象,利用苗期植株相对生长量进行耐盐性鉴定。【结果】148个导入系材料的平均盐害系数为61.22%,耐盐性差异较大;以2个亲本的盐害系数为界线,将盐害系数分成3组,盐害系数0~29.35%的占23.65%,29.35%~71.70%的占39.19%,71.70%~100%的占37.16%。【结论】通过对耐盐性状的QTL(Quantitative trait locus)定位,检测到了23个耐盐相关的QTL,其中12个耐盐QTL分布在A05、A12、D05和D11染色体上,说明从陆地棉背景的海岛棉染色体片段导入系中进行耐盐材料筛选是可行的,对特定染色体的筛选会具有更高的效率。

关键词: 棉花; 染色体片段导入系; 耐盐性; QTL定位

Abstract:

[Objective] Tolerance evaluation is the basis for cotton salinity-resistant breeding. [Method] Relative growth of cotton plants at seedling stage for 148 introgression lines was used to evaluate the tolerance to salinity. [Result] The results demonstrated that salt damage coefficients were significantly different from each other, with an average 61.22% ranging from 1.34% to 100%. Based on the salt damage coefficient, 148 introgression lines were divided into three groups; group 1 (23.65%) was more tolerant than the tolerant parent with the salt damage coefficient less than 29.35%; group 2 (39.19%) was more sensitive than the tolerant parent with the salt damage coefficient from 29.35%―71.70%; group 3 (37.16%) was more sensitive than the susceptible parent with the salt damage coefficient above 71.70%. [Condusion] Mapping of QTLs (Quantitative trait locus) for salt-tolerant traits indicated that 23 QTLs for tolerance-related traits were detected. Among them, 12 QTLs were aggregately distributed on chromosomes A05, A12, D05 and D11. Our results demonstrate that it is feasible for screening salt-tolerant introgression lines from Gossypium barbadense into G. hirsutum, especially by molecular marker-assisted selection for specific chromosomes with high efficiency.

Key words: cotton; chromosome segment introgression line; tolerance to salinity; QTL mapping