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棉花学报 ›› 2019, Vol. 31 ›› Issue (3): 169-181.doi: 10.11963/1002-7807.zsywsf.20190412

• 研究与进展 •    下一篇

四倍体棉花GAE基因家族的鉴定及其在棉纤维发育中的表达分析

张松雨(),王敬敬,刘正文,张艳,杨君,马峙英,王省芬*()   

  1. 河北农业大学/教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室,河北 保定 071001
  • 收稿日期:2018-02-28 发布日期:2019-05-29
  • 通讯作者: 王省芬 E-mail:303029052@qq.com;cotton@hebau.edu.cn
  • 作者简介:张松雨(1991―),女,硕士, 303029052@qq.com
  • 基金资助:
    国家转基因生物新品种培育科技重大专项(2016ZX08005-003);河北农业大学作物学科梯队建设基金(TD2016C201)

Genome-wide Identification and Expression Analysis of GAE Gene Family in Fiber Developmental Stages of Tetraploid Cotton

Zhang Songyu(),Wang Jingjing,Liu Zhengwen,Zhang Yan,Yang Jun,Ma Zhiying,Wang Xingfen*()   

  1. Hebei Agricultural University /North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory for Crop Germplasm Resources of Hebei, Baoding, Hebei 071000, China
  • Received:2018-02-28 Published:2019-05-29
  • Contact: Wang Xingfen E-mail:303029052@qq.com;cotton@hebau.edu.cn

摘要:

【目的】通过对陆地棉及海岛棉GAE基因家族进行全基因组分析,为深入研究GAE基因家族在棉纤维发育中的功能提供理论依据。【方法】利用生物信息学分析软件,对GAE基因家族进行全基因组鉴定,根据转录组数据分析在纤维发育过程中的表达规律。【结果】陆地棉GhGAE家族包含21个成员,海岛棉GbGAE家族有22个成员,分为3个亚组,多数GhGAEsGbGAEs基因没有内含子,共分布在12条染色体上。GAE氨基酸序列保守基序有4个,保守性较强,且GAE蛋白均定位于高尔基体膜。根据GAEs在陆地棉、海岛棉纤维发育不同时期的表达变化,将其分为纤维起始期高表达、纤维伸长期高表达、次生壁增厚期高表达、全时期低表达4类模式。其中GhGAE01、GhGAE02、GhGAE11、GhGAE12在陆地棉中高水平表达,GbGAE01、GbGAE02、GbGAE11、GbGAE12在海岛棉中高水平表达,推测这些基因可能在纤维发育过程中发挥着重要作用。【结论】上述结果为研究GAE基因家族在棉纤维发育中的功能提供了参考依据。

关键词: 陆地棉; 海岛棉; 葡糖醛酸异构酶; 生物信息学; 纤维发育; 基因表达

Abstract:

[Objective] The UDP-D-glucuronate 4-epimerase (GAE) families of Gossypium hirsutum L. and G. barbadense were investigated from whole genomes to provide a theoretical basis for further studies regarding the GAE family’s function in cotton fiber development. [Methods] The GAE family was identified using bioinformatics analysis software, and the expression pattern during fiber development was analyzed using transcriptional data. [Results] In total, 21 and 22 GAE genes were systematically identified from G. hirsutum and G. barbadense, respectively. They were classified into three subfamilies. Most of the GhGAEs and GbGAEs had no introns. They co-distributed over 12 chromosomes and contained four conserved motifs. All of the GAE proteins were predicted to localize to the Golgi membrane. On the basis of changes in the GAEs’ expression levels at different stages of fiber development, GAE proteins were divided into four categories, high expression at the initial stage, high expression at the fiber elongation stage, high expression at the secondary wall thickening period and low expression during the whole period. GhGAE01, -02, -11 and -12 and GbGAE01, -02, -11 and -21 were highly expressed in G. hirsutum and G. barbadense, respectively. These genes might play important roles in fiber development. [Conclusion] These results provide useful references for studying the GAE gene family’s function in cotton fiber development.

Key words: Gossypium hirsutum; Gossypium barbadense; UDP-D-glucuronate 4-epimerase; bioinformatics; fiber development; gene expression