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棉花学报 ›› 2017, Vol. 29 ›› Issue (6): 495-503.doi: 10.11963/1002-7807.znhy.20170913

• 研究与进展 • 上一篇    下一篇

陆地棉MIKCC基因家族的全基因组分析

周娜,汪露瑶,张天真,胡艳*   

  1. 南京农业大学作物遗传与种质创新国家重点实验室,南京 210095
  • 收稿日期:2017-04-17 出版日期:2017-11-15 发布日期:2017-11-15
  • 通讯作者: njauhuyan@163.com
  • 作者简介:周娜(1990―),女,硕士,2014101153@njau.edu.cn
  • 基金资助:
    中央高校基本科研业务费自主创新重点研究项目(2015001)

Genome-Wide Analysis of MIKCC Gene Family in Cotton

Zhou Na, Wang Luyao, Zhang Tianzhen, Hu Yan*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2017-04-17 Online:2017-11-15 Published:2017-11-15

摘要: 【目的】MIKCC是1类保守的转录因子家族,参与调控植物的开花时间和花器官发育。通过对MIKCC家族进行全基因组学分析,为深入研究MIKCC在棉花开花及花器官发育的分子调控机理提供基础。【方法】利用HMMER 3.0及pfam种子文件鉴定棉花全基因组MIKCC基因,结合其表达量进行偏向表达及聚类分析。【结果】在陆地棉基因组中共鉴定发现100个MIKCC基因,分为11个亚类。表达聚类分析显示,棉花MIKCC基因的表达模式大致可分为4个不同的类群,说明这类基因在棉花进化中出现了功能分化。100个MIKCC基因中,有12个基因具有miRNA靶位点。【结论】研究结果显示MIKCC家族基因在棉花纤维中存在功能分化,而且可能受到miRNA的调控,这为进一步研究该家族基因的功能提供信息参考。

关键词: 陆地棉; MIKCC基因; 功能分化; miRNA

Abstract: [Objective] In plants, floral organ identity and the process of the transition from vegetative to reproductive development is regulated by MIKCC gene family. Whole genome analysis is helpful to understand the evolutionary history of MIKCC genes and provides the basis for an in-depth analysis of molecular mechanism of MIKCC family in flowering and floral organ development in cotton. [Method] Whole genome identification of MIKCC genes were carried by HMMER 3.0 and pfam gene bias expression and were clustered based on the RNA-seq data. [Result] We identified 100 MIKCC genes and classified them into 11 subfamilies, according to their phylogenetic relationships to the Arabidopsis and Vitis vinifera MIKCC genes. According to their expression profiles, MIKCC genes were classified into four types. The MIKCC genes of C/D and E subfamily were particularly highly expressed during cotton fiber elongation stage, indicating gene functional differentiation in cotton evolution. 12 of the 100 MIKCC genes contains miRNA target sites, indicating that the flower development and the transition from vegetative to reproductive development of cotton may be regulated by miRNAs. [Conclusion] This study not only showed the functional differentiation of MIKCC genes in cotton fiber development, but also found that MIKCC family genes may be regulated by miRNAs in the growth and development of cotton, which provided the useful information for further functional studies of MIKCC genes in cotton.

Key words: upland cotton; MIKCC gene; functional differentiation; miRNA

中图分类号: 
  • S562.03