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棉花学报 ›› 2018, Vol. 30 ›› Issue (3): 205-214.doi: 10.11963/1002-7807.zbzxy.20180418

• 研究与进展 •    下一篇

过表达拟南芥AtCIPK 23、AtAKT 1和AtCBL 1基因棉花苗期耐低钾的差异及机制研究

张斌#,刘记#,张朝军,孔德培,王鹏,杨召恩,李付广*,张雪妍*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2017-10-16 出版日期:2018-05-15 发布日期:2018-05-15
  • 通讯作者: 李付广,aylifug@163.com;张雪妍,zhangxueyan@caas.cn
  • 作者简介:张斌(1992-),男,硕士研究生,18837299076@163.com;刘记(1989-),男,博士研究生,liuji@caas.cn;#同等贡献
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项(1610162016011);国家重点研发计划-棉花品质、抗逆等重要性状的功能基因组与调控网络(2016YFD0101006)

The Difference and Mechanism in Tolerance to Low-Potassium among Cotton Seedlings Over-Expressed Arabidopsis AtCIPK 23, AtAKT 1 and AtCBL 1 Genes

Zhang Bin#, Liu Ji#, Zhang Chaojun, Kong Depei, Wang Peng, Yang Zhao’en, Li Fuguang*, Zhang Xueyan*   

  1. State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China
  • Received:2017-10-16 Online:2018-05-15 Published:2018-05-15

摘要: 目的】评价过表达拟南芥AtCIPK 23、AtAKT 1和AtCBL 1基因棉花苗期耐低钾能力的差异,为棉花钾高效品种培育提供理论依据。【方法】通过室内水培法,测定比较了转基因棉花和中棉所24(CCRI 24)在适钾、低钾条件下苗期的长势、干物质质量、钾浓度和钾累积量等指标,并从根系形态、钾利用指数、钾转运能力和受胁迫程度等方面综合分析了其可能的生理机制。【结果】适钾条件下,转基因棉花和中棉所24长势基本一致,无显著差异。低钾胁迫时,过表达AtAKT 1基因棉花幼苗总根长、根表面积和根体积约为中棉所24的3倍,整株干物质质量是中棉所24的1.66倍,具有明显的生长优势,并且钾转运能力强、钾利用指数高,受胁迫程度明显降低;而过表达拟南芥AtCIPK 23和AtCBL 1基因棉花幼苗的各项指标与中棉所24相当,无显著差异。【结论】在棉花中过表达拟南芥钾离子通道蛋白基因AtAKT 1能显著提升棉花本身的耐低钾能力。

关键词: 中棉所24; 苗期; 耐低钾; 钾利用; 胁迫

Abstract: [Objective] To provide theoretical basis for potassium efficient cotton breeding, this work aimed to evaluate the influence of low potassium on transgenic cotton seedlings over-expressing Arabidopsis thaliana’s AtCIPK 23, AtAKT 1 and AtCBL 1 genes, respectively. [Method] Hydroponics was used to explore the growth vigor, dry weight, potassium concentration and accumulation of transgenic cotton and CCRI 24 seedlings under low and optimum potassium conditions. Furthermore, the root morphology, potassium utilization index, potassium transport ability and the severity to damage were comprehensively analyzed to uncover the possible physiological mechanisms. [Result] Under the optimum conditions of potassium, the transgenic plants and control grew well and showed no significant difference. However, with low potassium stress, the dry weight of plants over-expressing AtAKT 1 gene was 1.66 times of CCRI 24. Its total root length, root surface area and volume were about 3 times than those of CCRI 24. The AtAKT 1 plants also displayed obvious growth advantage, higher potassium utilization index and transport ability, and the severity to damage was lower. Plants over-expressing AtCIPK 23 and AtCBL 1 genes, however, were almost the same as the CCRI 24. [Conclusion] This study revealed that the ability of cotton’s resistance to low potassium was significantly improved by over-expression of AtAKT 1 gene, an exogenous potassium ion channel protein gene from Arabidopsis thaliana.

Key words: CCRI 24; seedling; tolerance to low-potassium; potassium utilization; stress

中图分类号: 
  • S562.01