Please wait a minute...

   检索  |  高级检索
棉花学报  2018, Vol. 30 Issue (3): 231-241    DOI: 10.11963/1002-7807.xyczzl.20180430
  研究与进展 本期目录 | 过刊浏览 | 高级检索 |
复合盐碱胁迫下半野生棉苗期耐盐性综合评价及其关键生理指标的变化
许艳超,韦洋洋,李振庆,蔡小彦,王玉红,王星星,张振梅,王坤波,刘方*,周忠丽*
棉花生物学国家重点实验室/中国农业科学院棉花研究所,河南 安阳 455000
Integrated Evaluation and the Physiological and Biochemical Responses of Semi-Wild Cotton under Complex Salt-Alkali Stress
Xu Yanchao, Wei Yangyang, Li Zhenqing, Cai Xiaoyan, Wang Yuhong, Wang Xingxing, Zhang Zhenmei, Wang Kunbo, Liu Fang*, Zhou Zhongli*
State Key Laboratory of Cotton Biology / Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China
全文: PDF(1567 KB)  
输出: BibTeX | EndNote (RIS)      
摘要 【目的】半野生棉遗传多样性丰富,具有较多优异的抗性基因源。本研究旨在初步了解半野生棉对新疆棉田复合盐碱胁迫的调控机理,探索室内鉴定评价半野生棉复合盐碱抗性的方法。【方法】本文选用陆地棉6份材料,水培鉴定复合盐碱胁迫下的表型,同时测定生物量和一些相关的生理生化指标。采用主成分赋权灰色模型分析、聚类分析和方差分析等方法综合评价苗期的耐盐性;采用相关性分析、灰色关联度分析等方法研究苗期生理生化响应机理。【结果】表型观测发现棉花新生叶片损伤程度较高,阔叶棉130叶片损伤程度高于其他棉种。主成分赋权灰色模型综合评价表明6份棉花材料的复合盐碱抗性顺序为:玛利加朗特85>阔叶棉32 >中棉所16>中棉所12>阔叶棉40>阔叶棉130。生理生化分析表明活性氧清除是盐碱胁迫下重要的响应系统。研究也发现胁迫48 h内,盐敏感与耐盐两类材料的响应程度不同,包括根中的抗坏血酸过氧化物酶、过氧化氢酶、叶中的过氧化物酶活性具有不同的变化趋势。【结论】研究证明了室内鉴定评价半野生棉复合盐碱抗性的方法准确可信,揭示了活性氧平衡系统是棉花调控复合盐碱胁迫响应的重要系统。同时本研究为棉花盐碱抗性机理的研究、耐盐碱材料的选育、内源抗性基因的挖掘提供了材料和理论依据。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
许艳超
韦洋洋
李振庆
蔡小彦
王玉红
王星星
张振梅
王坤波
刘方
周忠丽
关键词 半野生棉苗期复合盐碱抗盐性    
Abstract:[Objective] The genetic diversity of semi-wild cotton was abundant, so more new elite resistant genes could be found and applied to improve the resistance of cotton. The aim of this study was to primarily study on the regulation of response to salt-alkali stress, explore the method of integrated evaluation and characterize the resistance of semi-wild cotton. [Method] Four semi-wild and two cultivated Gossypium hirsutum accessions were evaluated for phonotypical, physiological and biochemical traits under alkali-salt versus normal conditions in hydroponic solutions. The alkali-salt tolerance was determined based on principal component analysis, cluster analysis, correlation analysis, grey rational analysis and analysis of variance. [Result] On the basis of the overall results, the alkali-salt stress tolerance of the six accessions was ranked as: Marie-galante85 > Latifolium32 > CRI16 > CRI12 > Latifolium40 > Latifolium130. Roots were found to be the most important responsive systems to complex alkali-salt stress. At seedling growth stage, the active scavenging system played a crucial role in response to alkali-salt stress. In addition, the salt tolerant and salt sensitive accessions showed different response tends towards leaf peroxidase, root ascorbate peroxidase and root catalase within 48 h, suggesting the accessions have different levels of response to salt stress. [Conclusion] Our study identified the most alkali-salt tolerant accessions and provided basic concept of complex alkali-salt tolerance mechanism within cotton accessions. And, our study provided a simple and rapid, highly accurate and precise method for evaluating salt resistance of cotton, and proved that the balance of ROS system play an important role in response to salt-alkali stress. Hence, mining of salt tolerant genes from these accessions may facilitate the development of novel salt tolerant variety.
Key wordssemi-wild cotton    seeding stage    complex salt-alkali    salt tolerance
收稿日期: 2016-12-29      出版日期: 2018-06-07
中图分类号:  S562.01  
基金资助:国家自然科学基金重点项目——半野生棉对新疆次生盐碱的应答机制及应用(31530053)
*通信作者: 周忠丽,zhonglizhou@163.com;刘方,liufcri@163.com   
作者简介: 许艳超(1991―),男,硕士,xuyanchao2016@foxmail.com;韦洋洋(1987―),男,博士,weiyangyang511@126.com
引用本文:   
许艳超,韦洋洋,李振庆, 等. 复合盐碱胁迫下半野生棉苗期耐盐性综合评价及其关键生理指标的变化[J]. 棉花学报, 2018, 30(3): 231-241.
Xu Yanchao,Wei Yangyang,Li Zhenqing, et al. Integrated Evaluation and the Physiological and Biochemical Responses of Semi-Wild Cotton under Complex Salt-Alkali Stress[J]. Cotton Science, 2018, 30(3): 231-241.
链接本文:  
http://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.xyczzl.20180430      或      http://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2018/V30/I3/231
<p>[1] 王遵亲, 祝寿泉, 俞仁培. 中国盐渍土[M]. 北京: 科学出版社, 1993.<br />
Wang Zunqin, Zhu Shouquan, Yu Renpei, et al. The saline soil in China[M]. Beijing: Science Press, 1993.<br />
[2] 彭镇华. 中国森林生态网络体系建设[M]. 北京: 中国林业出版社, 2002.<br />
Peng Zhenhua. Forest eco-network system in China[M]. Beijing: China Forestry Publishing House, 2002.<br />
[3] 彭镇华, 王成. 河流沿线土地利用对策的研究[J]. 应用生态学报, 2002, 13(4): 481-485.<br />
Peng Zhenhua, Wang Cheng. Land-use strategy in riverine area[J].Chinese Journal of Applied Ecology, 2002, 13(4): 481-485.<br />
[4] 罗廷彬,任崴,谢春虹.新疆盐碱地生物改良的必要性与可行性[J].干旱区研究,2001,18(1):46-47.<br />
Luo Tingbin, Ren Wei, Xie Chunhong. Necessity and feasibility of biotic improving the saline and alkaline land in Xinjiang[J]. Arid Zone Research, 2001, 18(1): 46- 47.<br />
[5] Wang W, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance[J]. Planta, 2003, 218(1): 1-14.<br />
[6] Teow C C, Truong V D, McFeeters R F, et al. Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours[J]. Food Chemistry, 2007, 103(3): 829-838.<br />
[7] 赵学良, 张彦才. NaCl胁迫对棉花苗期营养元素吸收与含量的影响[J]. 河北农业大学学报, 1992, 15(2): 41-44.<br />
Zhao Xueliang, Zhang Yancai. Influence of NaCl stress on the uptake and concentrations of nutriments in cotton seedlings[J]. Journal of Hebei Agricultural University, 1992, 15(2): 41-44.<br />
[8] 陈亚华, 沈振国, 刘友良, 等. NaCl胁迫下棉花幼苗的离子平衡[J]. 棉花学报, 2001, 13(4): 225-229.<br />
Chen Yahua, Shen Zhenguo, Liu Youliang, et al. Ion homeostasis in NaCl-stressed cotton[J]. Cotton Science, 2001, 13(4): 225-229.<br />
[9] Gouia H, Ghorbal M H, Touraine B. Effects of NaCl on flows of N and mineral ions and on NO3-reduction rate within whole plants of salt-sensitive bean and salt-tolerant cotton[J]. Plant Physiology, 1994, 105(4): 1409-1418.<br />
[10] 辛承松, 唐薇, 王洪征, 等. 鲁棉 14 幼苗生长对氯化钠胁迫的反应及微量元素, 激素处理的效应[J]. 棉花学报, 2002, 14(2): 108-112.<br />
Xin Chengsong, Tang Wei, Wang Hongzheng, et al. Responses od seedling growth of Lumian14 to NaCl stress and effects of treatment with microelement and hormone[J]. Cotton Science, 2002, 14(2): 108-112.<br />
[11] 沈法富, 尹承佾. 棉花植株和花粉耐盐性的鉴定[J]. 作物学报, 1997, 23(5): 620-625.<br />
Shen Fafu, Yin Chengyi. Screening of whole plants and pollen grains of cotton for salt tolerance[J]. Acta Agronomica Sinica, 1997, 23(5): 620-625.<br />
[12] Khan A N, Qureshi R H, Ahmad N. Salt tolerance of cotton cultivars in relation to relative growth rate in saline environments[J]. International Journal of Agriculture & Biology, 2004, 6(5): 786-787.<br />
[13] 戴海芳, 武辉. 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选[J]. 中国农业科学, 2014, 47(7): 1290-1300.<br />
Dai Haifang, Wu Hui. Analysis of salt-tolerance and determination of salt-tolerant evaluation indicators in cotton seedlings of different genotypes[J]. Scientia Agricultura Sinica, 2014, 47(7): 1290-1300.<br />
[14] 周忠丽, 蔡小彦, 王春英, 等. 半野生棉耐盐碱筛选初报[J]. 中国棉花, 2015, 42(1): 15-18.<br />
Zhou Zhongli, Cai Xiaoyan, Wang Chunying, et al. Primary report of the tolerance identification to saline on Gossypium hirsutum races[J]. China Cotton, 2015, 42(1): 15-18.<br />
[15] 刘雅辉, 王秀萍, 张国新,等. 棉花苗期耐盐生理指标的筛选及综合评价[J]. 中国农学通报, 2012, 28(6):73-78.<br />
Liu Yahui, Wang Xiuping, Zhang Guoxin, et al. Study on selection of physiological indices for salt tolerance and comprehensive evaluation of cotton during seeding stage[J]. Chinese Agricultural Science Bulletin, 2012, 28(6): 73- 78.<br />
[16] 刘雅辉, 王秀萍, 张国新,等. 棉花苗期耐盐形态指标的筛选及综合评价[J]. 安徽农业科学, 2012, 40(9):5119-5120.<br />
Liu Yahui, Wang Xiuping, Zhang Guoxin, et al. Selection of morphology indices for salt tolerance and comprehensive evaluation of cotton during seeding stage[J]. Journal of Anhui Agricultural, 2012, 40(9): 5119- 5120.<br />
[17] 彭振, 何守朴, 孙君灵,等. 陆地棉苗期耐盐性的高效鉴定方法[J]. 作物学报, 2014, 40(3):476-486.<br />
Peng Zhen, He Shoupu, Sun Junling, et al. An efficient approach to identify salt tolerance of upland cotton at seeding stage[J].Acta Agronomica Sinica, 2014, 40(3): 476- 486.<br />
[18] 张子学, 王正鹏, 凌中鑫,等.不同棉花品种NaCl胁迫的生理表现及其耐盐性筛选指标分析[J]. 中国棉花, 2004, 31(8):8-10.<br />
Zhang Zixue, Wang Zhengpeng, Ling Zhongxin, et al. The physiological and indices election analysis of different cotton varieties for salt tolerance[J]. China Cotton, 2004, 31(8): 8-10.<br />
[19] 叶武威, 刘金定. 棉花种质资源耐盐性鉴定技术与应用[J]. 中国棉花, 1998, 25(9): 37-38.<br />
Ye Wuwei, Liu Jinding. The technology and application of salt resistance identifying of cotton germplasm resources[J]. China Cotton, 1998, 25(9): 37- 38.<br />
[20] Giannopolitis C N, Ries S K. Superoxide dismutases: I. Occurrence in higher plants[J]. Plant Physiology, 1977, 59(2):309<br />
[21] Hodgcs D M,Andrews C J, Johnson D A, et al. Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines[J]. Plant Physiology, 1996, 98: 685-692.<br />
[22] Griffith O W. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine[J]. Annual Review of Biochemistry, 1980, 106: 207-212.<br />
[23] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts[J]. Plant and Cell Physiology, 1981, 22: 867-880.<br />
[24] 李合生. 植物生理生化试验原理和技术[M]. 北京:高等教育出版社, 2000.<br />
Li Hesheng. Plant physiological and biochemical experiment principle and technology[M]. Beijing: Higher Education Press, 2000.<br />
[25] 陈书琴, 许秋瑾, 颜昌宙, 等. 主成分分析赋权及其在水质评价灰色模型中的应用[J]. 昆明理工大学学报(理工版), 2008, 33(2): 77-80.<br />
Chen Shuqin, Xu Qiujin, Yan Changzhou, et al. Application of principal components analysis method to weight determination in fuzzy recognition model for water quality assess[J]. Journal of Kunming University of Science and Technology (Science and Technology), 2008, 33(2): 77-80.<br />
[26] 叶武威, 庞念厂, 王俊娟, 等. 盐胁迫下棉花体内 Na+ 的积累, 分配及耐盐机制研究[J]. 棉花学报, 2006, 18(5): 279-283.<br />
Ye Wuwei, Pang Nianchang, Wang Junjuan, et al. Characteristics of absorbing, accumulating and distribution of Na+ under the salinity stress on cotton[J]. Cotton Science, 2006, 18(5): 279-283. <br />
[27] Payton P, Webb R, Kornyeyev D, et al. Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity[J]. Journal of Experimental Botany, 2001, 52(365): 2345-2354.<br />
[28] 蒋明义, 郭绍川. 氧化胁迫下稻苗体内积累的脯氨酸的抗氧化作用[J]. 植物生理学报, 1997, 23(4): 347-352.<br />
Jiang Mingyi, Guo Shaochuan. Proline accumulation in rice seedling exposed to oxidative stress in relation to antioxidation[J]. Acta Phytophysiologica Sinica, 1997, 23(4): 347-352. <br />
[29] Sairam R K, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants[J]. Current Science, 2004, 86(3): 407-421.<br />
[30] 李付广, 李凤莲. 盐胁迫对棉花幼苗保护酶系统活性的影响[J]. 河北农业大学学报, 1994, 17(3): 52-56.<br />
Li Fuguang, Li Fenglian. Effects of salt stress on the activity of protective enzymes in cotton seedling[J]. Journal of Hebei Agricultural University, 1994, 17(3): 52-56.<br />
[31] Mittler R, Vanderauwera S, Gollery M, et al. Reactive oxygen gene network of plants[J]. Trends in Plant Science, 2004, 9(10): 490-498.<br />
[32] 吴晓东, 王巍, 金路路, 等. 盐胁迫对棉花光合作用和生理指标的影响[J]. 中国棉花, 2013, 40(6): 24-26.<br />
Wu Xiaodong, Wang Wei, Jin Lulu, et al. The effects of salt stress on photosynthesis and physiological indexes of cotton varieties[J]. China Cotton, 2013, 40(6): 24-26.</p>
[1] 张斌, 刘记, 张朝军, 孔德培, 王鹏, 杨召恩, 李付广, 张雪妍. 过表达拟南芥AtCIPK 23、AtAKT 1和AtCBL 1基因棉花苗期耐低钾的差异及机制研究[J]. 棉花学报, 2018, 30(3): 205-214.
[2] 张希鹤, 李岩, 郁凯, 霍钰阳, 王友华, 陈兵林. 黄萎病胁迫影响棉花幼苗光合及叶绿素荧光特性的机理[J]. 棉花学报, 2018, 30(2): 136-144.
[3] 国秀丽 , 白灯莎·买买提艾力, 张少民, 孙良斌, 冯固. AM真菌对苗期棉花根系形态特征的影响[J]. 棉花学报, 2017, 29(5): 476-486.
[4] 郑巨云,George Oluoch,Khan Muhammad Kashif Riaz,周忠丽,王星星,蔡小彦,李雪源,王春英,王玉红,刘方,王坤波. 毛棉苗期抗旱性状的QTL定位[J]. 棉花学报, 2017, 29(1): 29-39.
[5] 王园园, 刘方, 周忠丽, 蔡小彦, 王玉红, 黎绍惠, 王春英, 张香娣, 王星星, 王坤波. 半野生棉与亚洲棉对草甘膦抗性评价及比较分析[J]. 棉花学报, 2016, 28(2): 95-103.
[6] 李晓娜, 蔡小彦, Kiflom Weldu Okubazghi, 王星星, 王春英, 刘方, 周忠丽, 李诚, 王玉红, 祝水金, 彭仁海, 王坤波. 半野生棉棉仁含油量与SSR标记的关联分析[J]. 棉花学报, 2015, 27(2): 104-110.
[7] 肖水平, 吴香华, 孙亮庆, 刘新稳, 曾小林, 杨绍群, 柯兴盛. 棉花苗期钾营养效率的基因型分类及钾营养特性差异分析[J]. 棉花学报, 2014, 26(6): 546-554.
[8] 李 飞, 毛树春, 韩迎春, 范正义, 王国平, 李小新, 刘 芳. 遮阴对基质育苗裸苗移栽棉花苗期生长的影响[J]. 棉花学报, 2013, 25(1): 30-36.
[9] 刘 芳, 毛树春, 韩迎春, 李亚兵, 范正义, 王国平, 朱巧玲, 冯 璐, 董春旺. 不同增温处理对基质育苗移栽棉缓苗期部分生理生化指标的影响[J]. 棉花学报, 2011, 23(4): 341-346.
[10] 刘小玲, 毛树春, 韩迎春, 李亚兵, 范正义, 王国平, 冯 璐, 董春旺, 朱巧玲. 棉花三种育苗移栽新方法缓苗期棉苗若干生理生化的比较[J]. 棉花学报, 2010, 22(5): 437-442.
[11] 崔爱花, 毛树春, 韩迎春, 李亚兵, 范正义, 王国平, 王香河. 裸苗移栽棉花缓苗期抗氧化酶活性和叶绿素含量的变化特点[J]. 棉花学报, 2008, 20(5): 372-378.
[12] 侯 静, 盛建东, 李雪妮, 陈波浪. 棉花苗期钾营养高效品种筛选[J]. 棉花学报, 2008, 20(2): 158-封三.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed